Báo cáo khoa học: Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin pdf
Báo cáo khoa học: Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin pdf
... Journal 274 (2007) 677–686 ª 2006 The Authors Journal compilation ª 2006 FEBS Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin Joa˜o B. Vicente1, ... electrons at the site of reactionwith NO, this prompted us to use E. coli FlRd as amodel to study the kinetics of electron transfer (eT)along the NADH fi FlRd -reductase fi FlRd chainRd NADH NAD+FADeNON2OFMNFe-FeFlavodiiron ... CoreFlRd -reductase FlavorubredoxinFig. 1. Schematic representation of the Escherichia coli electron transfer chain coup-ling NADH oxidation to NO reduction. Electron transfer to E. coli flavorubredoxin...
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Báo cáo khoa học: Interactions of elongation factor EF-P with the Escherichia coli ribosome doc
... deter-mined, as was the area of each peak. The ratio of the density, which is related to the amount of EF-P bound, to the area of the peaks, which is related to the totalamount of the ribosome population ... required to accommodate fMet–tRNA in the P-site of the 70S ribosome.A computer-simulated structure of the 70S ribosome of E. coli was used to fit the protected bases of the EF-P protein relative to the ... decoding of the mRNA transcript. For example, by binding to the A-site, EF-P may prevent the incorrect position-ing of the fMet-RNA into the A-site of the 50S sub-unit. The binding site of EF-P to...
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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 fi 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf
... After the concentrations of the resultant glucose and fructose were measured, the extent of sucrose splitting was determined from the amount of fructose released, and the extent of glucosyl transfer ... addition, the kinetics of the acceptor reactions appeared to be similar betweenGS and GSGB.Kinetic simulation of GTF glucan synthesis To explain the time course of saccharide productionduring the ... and (e) the final yield of saccharide was similar to that of the experimentaldata, even in terms of the total concentration of soluble nigerooligosaccharides (DP = 2–6). In otherwords, the difference...
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
... muchslower than the rate of deacylation. The theoretical maxi-mum of the amplitude of the burst phase is equal to the totalenzyme concentration. When kh1¼ 5 k2, only 2% of the maximum of the amplitude ... proton that gives rise to the normal isotopeeffect may be the proton that is transferred from the seryloxygen to the seryl amino group during activation of the nucelophilic serine, or the proton ... mechanism, the proton inventory of the kcatforNIPAB showed that one proton, with a fractionation factor of 0.5, is transferred in the transition state of the rate-limitingstep. The overall KIE of...
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
... operating at varioussteps in the cytotoxic activity of the drug, from adecrease of drug accumulation in the cell to abrogation of apoptosis induced by the chemical substance. Oftenseveral different ... sensitivity of tumor cells to chemo-therapy and is linked to activation of the phosphatidylinositol-3-kinase/Akt(PI3K/Akt) pathway. In the present study, we further investigate the role of the PI3K/Akt ... exhibiting IR; 3, 50–75% of the tumor cells exhibiting IR; 4, > 75% of the tumor cellsexhibiting IR. The overall score was the multiplication of the two. The scores were then classified as negative...
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Báo cáo khoa học: Contributions of host and symbiont pigments to the coloration of reef corals pptx
... werecalculated from the peak absorptions of the bands at pH 7.0 to provide a measure of coloration.bData from [19].cData from [41].ABDCFig. 1. Comparison of spectral properties of the green -to- red ... imidazolylgroup of the chromophore as in other green -to- redphotoconverting proteins, due to a noncoplanararrangement of the imidazole moiety with the rest of the chromophore. Effects of tetramer ... way to the col-oration of M. cavernosa. The red fluorescent morphshowed high-level transcription of the green -to- red pho-toconvertible protein mcavRFP. In the green morph, the transcript of...
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Báo cáo khoa học: Inter-flavin electron transfer in cytochrome P450 reductase – effects of solvent and pH identify hidden complexity in mechanism potx
... the relevant organism [11–15]. Thus, the flavin cofac-tors mediate the successive transfer of two electrons from a two -electron donor, NADPH, to the obligatoryone -electron acceptor moiety (the ... in the experimental buffers used in this work might be suffi-cient to account for the discrepancies. Because elec-trons have to pass from the FAD to the FMNcofactor to be transferred to the ... withCPR, the relaxation to the thermodynamic equilibriumposition of the two -electron reduced species competeswith the reduction to the four -electron level by asecond molecule of NADPH. Because the...
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Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
... inhibitors of human neutrophil elastase andproteinase 3 but not of cathepsin G. Both inhibitors belong,together with secretory leukocyte protease inhibitor, to the chelonianin family of canonical ... protease(s). To further characterize the maturation of elafin from trappin-2 and to compare the antiproteolytic activity of both inhibitors, we haveexpressed them in the Pichia pastoris expression ... inhibitor (I), substrate (S), and their complexes are inthermodynamic equilibrium. The active concentration of both recombinant inhibitors was deduced from the volume of inhibitor necessary to totally...
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Báo cáo khoa học: Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers pptx
... new mathematical model [22] to analyse the kinetics of conversion of violaxanthin to zeaxanthin. The model allowed us to follow independently the kinetics of the two de-epoxidation steps: the conver-sion ... antheraxan-thin, formed in such domains, has an immediate access to the VDE enzyme. Therefore, and in contrast to the de-epoxidation of violaxanthin to antheraxanthin, the conversion of antheraxanthin ... dynamics of membrane regionsclose to the headgroup area. The spin label was added to the chloroform mixture of monogalactosyldiacylglycerol, Ptd-Cho and V, dried under stream of nitrogen and stored...
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Báo cáo khoa học: Characterization of SCP-2 from Euphorbia lagascae reveals that a single Leu/Met exchange enhances sterol transfer activity pdf
... aggregates) to the sample. If the added lipid was a substrate for SCP-2, itwould result in a decrease in the rate of transfer of BODIPY-PC because of the competition from the presence of a new ... resistant to the SCP-2-medi-ated lipid transfer as possible, and therefore the transfer protein preferentially transfers the labelledBODIPY-PC. To examine the ability of E. lagascaeSCP-2 to recognize ... His18 of A. thaliana SCP-2 into Phe18, andLys28 of E. lagascae SCP-2 into Glu28. The BODIPY-PC -transfer activity for the different mutants differedonly slightly from each other and from the...
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