... were
recalculated. The normalized B factors from apo importin-a
were then subtracted from those in the importin-a:CLIC4
NLS structure, and the difference was again normalized
using the equation above. The final ... exception to this is the
location of the amide group of Lys204 at P3, where
there is a break in the main-chain density at the 2.8r
map level. The corres...
... the superimpo-
sition of ApeSOD with the cambialistic SOD from
P. shermanii. The overall structures of these enzymes
were similar; the rmsd of the 747 Ca atoms was
0.695 A
˚
.
ApeSOD eluted from ... aerobic hyperthermophilic archaeon, produces a
cambialistic superoxide dismutase that is active in the presence of either of
Mn or Fe. The crystal structures of th...
... Thus, the differences between wild-type
and mutant structure are in the same range as the dif-
ferences between the two molecules of the asymmetric
unit of the wild-type structure. Neither the ... to determine the structure of the complex of an
inactive PhyK mutant with its substrate phytase, the refined
structure of the wild-type enzyme was used for mole...
... according to the residue in the mid-
point of the respective loop, as shown in Fig. 2. To
the east of the active site the 37- and 60-loops border
the S2¢ pocket of the proteinase. The observed ... below). The southern boundary of
the active site cleft of DESC1 is formed by the 145
autolysis loop. The backbone of this loop differs mark-
edly from the o...
... determines the shape of the
active site entry. (e) The structure of the active site is
essentially identical with the active sites of the MshB
and LpxC proteins. The conservation of catalytically
important ... diagram.
B
A
Fig. 1. Structure of the BcZBP dimer. (A) Topology diagram of the
dimer drawn with the program
TOPDRAW [34]. The relative orienta-
tio...
... However, the packing
of the two dimers in the tetramer is different from that of the
PDCs of known structure, best described as a 20° rotation
of one dimer towards the other when compared to the
Z. ... relative
orientation of the dimers in the tetramer is different in all of
the tetrameric PDCs of known three-dimensional structure.
Binding of the cof...
... report the crystal structure of the intact glu-
taminase under four different conditions: in the absence
of the additives (referred to as N); in the presence of
Tris (referred to as T); in the presence ... twofold axis of
crystallographic symmetry. The two independent
physiological dimers shared a similar structure. The
physiological dimer of the TG structure...
... part, and (b) the variability of the angle of
approach of the inhibitor relative to the catalytic
cleft of the enzyme. The latter factor may reflect not
so much the geometry of the catalytic site ... PW),
despite the lack of overall sequence similarity. The
role of the proline residue appears to be to maintain
the specific shape of the loop. The aroma...
... Crystal structure of the tetrameric inositol 1-phosphate
phosphatase (TM1415) from the hyperthermophile,
Thermotoga maritima
Kimberly A. Stieglitz
1
, Mary ... In the center there is a schematic
of the mutual relationship of the monomers,
in selected members of the family, as indi-
cated by the twist angle of the dimer.
TM1415 constitutes one...
... of w,fromthelistofsequencesgivenin
Table 3, is shown. The alignment is colored according to the degree of sequence conservation. These 26 residues are disordered in the crystal
structure of the ... but,
like the d and d¢ subunits of the clamp-loader, neither v
nor w contain any of the functional elements required for
nucleotide binding. The topology of the w...