Báo cáo khoa học: Intracellular degradation of somatostatin-14 following somatostatin-receptor 3-mediated endocytosis in rat insulinoma cells doc
Báo cáo khoa học: Intracellular degradation of somatostatin-14 following somatostatin-receptor 3-mediated endocytosis in rat insulinoma cells doc
... compilation ª 2008 FEBS 4739 Intracellular degradation of somatostatin-14 following somatostatin-receptor 3-mediated endocytosis in rat insulinoma cells Dirk Roosterman1, Nicole E. I. Brune2, ... Agonist-mediated endocytosis of rat somato-statin receptor subtype 3 involves beta-arrestin and clath-rin coated vesicles. J Neuroendocrinol 13, 279–287. Intracellular degradation of somatostatin D. Roosterman ... Distinct agonist-mediated endocyto-sis of cloned rat somatostatin receptor subtypes expressed in insulinoma cells. J Neuroendocrinol 9, 741–751.21 Lucius R & Mentlein R (1991) Degradation of...
- 12
- 375
- 0
Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
... cystatin C is the mostdiscriminating residue for binding to cathepsins B, H,L, S [24]. No L9K mutants of cystatin C have beenprepared yet to study the effect of incorporating theCystatin F in ... activation of cystatin F wehave studied the in vitro kinetics of the interactionbetween cystatin F and several cathepsins, as wellas their intracellular localization in promonocyteU937 cells, using ... the aminopeptidasecathepsin H (Ki¼ 30 nm ). In comparison with othercystatins, cystatin F is a rather slow binding inhibitor of the cathepsins, characterized by kassvalues in therange of...
- 11
- 621
- 0
Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx
... Regardless of dopamine treatment, overexpres-sion of a-synuclein significantly affected the levels of four proteins [stathmin 1, glutathione S-transferase(GST)p, Ran 1 binding protein and C1q binding ... suggested to be linked toearly stages of PD pathogenesis [26]. Interestingly, in our model, dopamine induces an increase of the actinbundles regulator fascin 1 [27] and discordant changes of two calcium-dependent, ... the level of a-synuclein in a-syn cells with respect to b-gal cells in the presence of catalase only (cat) or in the presence of catalase and0.250 mm dopamine for 24 h (DA). Dopamine treat-ment...
- 11
- 775
- 0
Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt
... also observed, includ-ing that Mxi1-SRa appears to be the predominanttranscript in the adult intestine and in the developingembryo, whereas Mxi1-SRb transcripts predominate in the adult liver ... Schematicrepresentation of the different Mxi1 isoforms tested for suppres-sive potential in the REF transformation assay. All of the isoformscarried Myc tags on their COOH termini. SID, Sin3 interactingdomain; ... and expanded. Afterinduction with doxycycline (1 lgÆmL)1), the individualclones were tested for their expression of the protein of interest.Flag pull down⁄silver staining⁄mass spectrometryanalysisFor...
- 11
- 586
- 0
Tài liệu Báo cáo khoa học: Down-regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism docx
... (HRP-labelled) (1 : 500)].Haematoxylin was employed for counterstaining.Haematoxylin–eosin stainingHaematoxylin–eosin staining was carried out following the routine histological method described ... absorptive site of folic acid, i.e. the smallintestine.Expression of the RFC protein in BBM of theintestineThe effect of chronic alcoholism on the level of expres-sion of the RFC protein at the ... protein)Fig. 2. [3H]Folic acid transport in intestinal BBMVs at differentintervals during the course of ethanol administration. An incubationbuffer of pH 5.5 and a [3H]folic acid concentration...
- 12
- 484
- 0
Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. SixGST-fused recombinant proteins containing the three distinctivedomains of tandem repeat ... Pi, and specificbinding of the antibody was detected by incubating thesections in a solution of 10 mm diaminobenzidine in 10 mm NaCl ⁄ Pi.H. Tohse et al. A novel protein in the otolith matrix ... otolith biomineralization. In the otolith matrix, OMM-64 was localized in ring-likestructures, indicating that OMM-64 is periodicallyincorporated into the otoliths. The manner of incorpo-ration...
- 12
- 568
- 0
Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc
... determination of the BcZBPprotein at a resolution of 1.8 A˚, provides importantclues towards understanding the enzyme function,including the identification of a zinc-binding site withina ... proteins. B. cereus thus provides a convenient model organism forstudying proteins potentially associated with the pathogenicity of the highlyinfectious B. anthracis. The zinc-binding protein of ... binding of acetate in the active site is afurther indication that the enzyme may be involved in deacetylation because acetate is one of the reactionproducts.Asp14, a residue of the loop joining...
- 11
- 710
- 0
Tài liệu Báo cáo khoa học: Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase) 6-phosphogluconolactonase docx
... effectsdetected at 96 h in cultures transfected with vectorscontaining or lacking an insert indicate that after 48 h,other interacting factors could modify the molecularphenotypic effect of using this vector. ... because of the import-ance of this enzyme in the parasite’s intraerythrocytestage [16]. The present paper describes the effects of G6PD-6PGL silencing in P. falciparum, confirming thekey role of ... enzyme in the intraerythrocyte stage of infection.ResultsEffects of PfG6PD-6PGL gene silencing on growthand parasite development In a first attempt at silencing the G6PD-6PGL gene,erythrocytes infected...
- 10
- 437
- 0
Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... fluorescence in HepG2 cells grown without (thin line, – Fe) or with (thick line, + Fe) iron in one representative of five separate experi-ments. Dotted line represents background staining in HepG2 cells ... (thick lines, + saponin) cells cul-tured without and with iron as indicated. (D) Perls’ staining of cyto-spins of HepG2 cells grown in media supplemented with 100 lM of ferric citrate for ... Dickinson, Mountain View, CA,USA). For intracellular staining, fixed cells in 2% (v ⁄ v) for-maldehyde were first permeabilized by incubation in NaCl ⁄ Pi⁄ 0.2% (w ⁄ v) saponin for 10 min....
- 14
- 682
- 0
Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis docx
... onplasmin(ogen) binding to fibrin and CHO-K1 cells, fibrinplates and cells were incubated with a fixed concentration of plasmin(ogen) supplemented with varying concentrations of mAbs. Bound plasmin(ogen) ... maximal rate of plasmin formation in theabsence of 6-Ahx. Data were fitted as indicated in Fig. 1 (IC50¼ 5mM). (B) Kinetics of plasmin formation performed on cells (main figure)and fibrin (inset) in ... lysine-bindingsite (LBS) of human plasminogen. Role of LBS in theregulation of fibrinolysis. Eur J Biochem 157, 65–69.48 Brockway WJ & Castellino FJ (1972) Measurement of the binding of...
- 14
- 558
- 0
Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ