Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt
Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt
... cross-linking efficiency of Iba1 and Iba2 or in the overall morphology of the generatedfilament bundles.Calcium affinity of Iba1 and Iba2 Homodimerization and actin binding of Iba1 and Iba2 were similar in ... presented here reveals functional simi-larities and differences between Iba1 and Iba2 . Weinvestigated Ca2+binding and homodimerization of Iba1 and Iba2 . Furthermore, F-actin binding and cross-linking ... may be concluded that EF-hand 1 of the Iba proteins is not capable of functional Ca2+binding.Function of EF-hand 2In both Iba1 crystal structures, the loops of EF-hand 2adopt essentially the...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... thatthe PRTFDC1 proteins have lost their phosphoribosyl-transferase activity [10].The structural characterization of numerous com-plexes of the human HPRT and several bacterial and protozoan ... authors.Studies of the human PRTFDC1 M. Welin et al.4930 FEBS Journal 277 (2010) 4920–4930 ª 2010 The Authors Journal compilation ª 2010 FEBS Structural and functional studies of the human phosphoribosyltransferase ... [13,14,17,19]. Human HPRT has been shown tobe a dimer or a tetramer in solution depending onionic strength [20].In the present study, we report the structural and functional characterization of the human...
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Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt
... D and E + F), (b) a few hydrogenbonds, and a hydrophobic interaction between A + C,B + E and D + F, and (c) electrostatic forces in thecentral channel of the hexamer between B, C and F, and ... ª 2007 FEBS Structural and functional investigations of Ureaplasmaparvum UMP kinase – a potential antibacterial drug targetLouise Egeblad-Welin1, Martin Welin2,*, Liya Wang1 and Staffan ... (1970) Cleavage of structural proteins dur-ing the assembly of the head bacteriophage T4. Nature227, 680–685.23 Bradford MM (1976) A rapid and sensitive method forthe quantitation of microgram...
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Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
... Isolation and characterization of adenovirus type2 vertex capsomer (penton base). Virology 92, 125–138.33 Pettersson U & Hoglund S (1969) Sructural proteins of adenoviruses. 3. Purification and characterization ... ionicstrength of the solvent environment and ⁄ or presence of small organic compounds (dioxane and MPD)seems to favor formation of dodecahedra over isolatedpentamers. Previous structural analysis of the ... is formed by an insertion of 230 residues(residues 133–367) between strands D and E and asmaller insertion of 50 residues (residues 404–458)between strands F and G. The hypervariable loop,Table...
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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf
... describes thecloning and functional characterization of an AK gene froma genomic library of L. donovani as well as its expression and molecular characterization. Materials and methodsMaterialsPlasmids ... transfer of the c-phosphate group from ATP toAMP, releasing two molecules of ADP. This study describescloning and functional characterization of the gene encodingAK2 from a genomiclibrary of Leishmania ... 29,24 and 12 h at temperatures of 4, 13 and 26.5 °C,respectively.Inhibition of AK by the specific inhibitor Ap5A and itseffect on growth of L. donovanipromastigotesin vitroThe effect of the...
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Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx
... longa-helices and a final b-strand, F, which protrudes fromthe core of the monomer and runs anti-parallel to thesame strand of a second monomer, allowing for theformation of the dimer. The surface of ... last class of cagPAI proteins, and summarize what is knownabout them both from a molecular and structural point of view, as well as their putative physiologicalroles.The unique members of the ... Helices B,E, F and H, arranged in an up -and- down topology,form the structural core, whereas the short helices C,D and G on one side, and A, I and J on the other,represent types of appendices,...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
... 2004Molecular and functional characterization of novel CRFR1 isoformsfrom the skinAlexander Pisarchik and Andrzej SlominskiDepartment of Pathology and Laboratory Medicine, University of Tennessee ... isoform where exon 3 and exon 6 are spliced out (U16273); CRFR1d isoform whereexons 6 and 13 are missing (AF180301); CRH-R1e withdeletion of exons 3 and 4 (AF369651); CRFR1f withdeletion of ... with deletion of exon 11, 27 basepairs of exon 10 and 28 basepairs of exon12 (AF369653); and CRFR1h with addition of a crypticexon (AF374231) [15–18]. In rodents, three CRFR1isoforms have already...
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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx
... Here we confirm and extend earlier studies of these AAA-peroxins and givea further detailed functional analysis of their cassettestructure and interaction.The interaction of Pex1p and Pex6p involvestheir ... biologicalfunction of Pex1p and Pex6p.ABFig. 2. Two-hybrid interaction of Pex1p and Pex6p harboring pointmutations of the WalkerA and B motifs of their ATP-binding sites.A schematic representation of the ... here demonstrate the inverseimportance of D1 and D2 in Pex1p and Pex6p for theinteraction of these two proteins. While a fragment of Pex6p consisting of N and D1 alone exhibits signifi-cant binding...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... transcript.Expression and biochemical characterization of VBARPThe predicted sizes of the proteins encoded byVBARP-L and VBARP-S are 69 and 49 kDa, respec-tively. To test this, C-terminal V5-tagged VBARP-L and ... Both VBARP-L and VBARP-S variantshave high homology to PP2500 and ANKHD1 vari-ant 2. In this study we focus on the biochemical and functional characterization of the novel VBARP-L and VBARP-S ... prosite motif scan and netphos software, also predicted the presence of several potential serine, threonine, and tyrosinephosphorylation sites (cAMP, PKC, and CK2), and the presence of nine potential...
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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf
... Comparative structures of CMP and UMP, and interactions between the cyto-sine moiety of nucleotide and various sidechains of wild-type CMP kinase. (Upper)Chemical differences between CMP and UMP are ... [17].ResultsOverproduction and molecular characterization of the modified variants of E. coli CMP kinaseThe wild-type and various modified forms (S36A,R110M, D132A, D132H, D132N, D132S and R188M) of E. coli ... reaction.Analysis of the effect of the mutations on proteinstability revealed three distinctive mechanisms of relaxation, and in the case of S36A, no relaxation atall. The first type of relaxation (structural...
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