... phosphorylated by a Golgi casein kinase- like
kinase ex vivo and circulates as a phosphoprotein
in humans
Thilina Dewpura
1,
*, Angela Raymond
1,
*, Jose
´
e Hamelin
2
, Nabil G. Seidah
2
, Majambu ... propeptide at Ser47 as
assessed by MS analysis of PCSK9 immunoprecipi-
tates in the presence and absence of shrimp alkaline
phosphatase (SAP). Radiola...
... compe-
tition experiments, increasing amounts (5, 10, 50, 100 and
200-fold excess) of unlabeled visfatin–PPREwt (5¢-CAAT
AC
AGGGCAAAGATCATGGAAG-3¢) or visfatin–PPRE-
mut (5¢-CAATAC
AGGAAAAAGAAAATGGAAG-3¢) ... -dependent manner in primary
human resting macrophages and in adipose tissue macrophages, but not in
adipocytes. The threefold increase of visfatin mRNA was paralleled by an...
... used a natural language tagger which was
trained on the output of ParaMor and Morfes-
sor. The goal was to mimic each algorithm since
ParaMor is rule-based and there is no access to
Morfessor’s internally ... goal of measuring how the learning
improves with increasing experience in terms of
training set size. We want to remind the reader that
our two algorithms are aimed at small...
... Base pairs
in the duplex exhibit slide ()1.96 A
˚
) and intermediate values
for X-displacement ()3.23 A
˚
), as in ADNA, while their
inclination to the helical axis is not prominent. Major and
minor ... Sundaralingam
3
and Narayanarao Yathindra
1
1
Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, India;
2
Department of Chemical
Sc...
... the putative
promoters of caspase-1, caspase-8 and caspase-10, and increase their expressions. In turn, increased pro-caspase-8 is recruited to HIPPI–HIP-1
heterodimer and increases apoptosis. The ... apoptosis by exogenous HIPPI
in the presence of endogenous HIP-1 is mediated
through activation of caspase-8, caspase-1, caspase-9 ⁄
caspase-6, and caspase-3. Cleavage of Bid...
... (forward 5¢-GAGATTGCACCACTGC
GCTCTA-3¢, reverse 5¢-AGCCAGAATGTCCCGTCAA
AAA-3¢) as a negative control.
Statistical analysis
All values for the luciferase activity assay are means ±
SEM. Data were analyzed ... atf3-sense,
5¢-GGTTTGCCATCCAGAACAAG-3¢; atf3-antisense, 5¢-CC
TCCCAGGAGAAGGTAAGC-3¢; adipor2-sense, 5¢-TAGC
CTTTGGTTTGCTTTGG-3¢; adipor2-antisense, 5 ¢-CATAT
CTCCAGGCGTCAACC-3¢; gapdh...
... chelatase and dehydrogenase function [17];
interestingly, this aspartate, Asp129, is also conserved
98
kDa
M Wt
Insoluble
Total cell lysate
Periplasm
Membrane
Cytoplasm
kDa
M Wt
Insoluble
Total ... conditions is not understood. Anal-
ysis of insertional mutagenesis and complementation
work in Pseudomonas aeruginosa, Pseudomonas fluores-
cens, Paracoccus denitrificans and Pseudomonas...
... of approaches to treat elastin-
degrading diseases, the aim of this study was to investigate the degradation
of the natural substrate tropoelastin by the elastinolytic matrix metallopro-
teinases ... metalloproteinase degradation of elastin,
type IV collagen and proteoglycan. A quantitative com-
parison of the activities of 95 kDa and 72 kDa gelatin-
ases, stromelysins-1 and -2 and...
... Escherichia coli DsbA and DsbC,
as well as the isolated catalytic a domain of PDI. Both
the PDI a domain and DsbA have a catalytic site, with
an associated substrate-binding site, but lack an inde-
pendent ... The Authors Journal compilation ª 2010 FEBS
bacitracin contains at least nine different peptides, of
which bacitracin A is the most abundant, and it is
mainly used...