Báo cáo khoa học: Structural ﬂexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for speciﬁc substrate recognition pptx

... FEBS Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition Silvija Bilokapic1, Jasmina Rokov Plavec1, Nenad Ban2 and ... consists of a long a-helical arm, and extensions in the N- and C-termini of the enzyme ensure recognition of twounusual mitochondrial tRNAsSer[11]. In general, the active sites of seryl-tRNA synthetases ... the spec-ificity of serine recognition depends on: (a) the zinc ion,(b) the size of the active site and (c) the hydrophilicnature of the serine-binding pocket. Despite precise recognition of...

Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx

... Asn51 and Cys54 (at the N-cap of the second helix); Ala64 and Ala65 (in the b-turn between the second and third helices); and Thr72 and Ala73 (at the C-cap of the third helix) (Fig. 4A). The ﬁrst ... in the monomeric structure of CAC, and (b) the inherent ﬂexibility for the majority of the residues. Thus, the conformational entropy appears tobe distributed through the whole structure of the ... Fersht AR (1995) Back-bone dynamics of chymotrypisn inhibitor 2: effect of breaking the active site bond and its implications for the mechanism of inhibition of serine proteases. Bio-chemistry...

Báo cáo khóa học: Structural properties of the protein SV-IV potx

... estimated that the a-helixcontent is 24% in the monomeric form and 45% in the trimeric form, and b-structure is absent from both forms. The CD spectra of the 1–70 and 70–90 regions of the proteinare ... in the trimericform is due to subunit association and the consequent loss of exposed surface. Arg57 and Arg81 may be located in the region of interaction, and therefore would be exposed in the monomeric ... exposed in the monomeric form and buried in the trimeric form. The second hypothesis is based on the observation of a highera-helix content in the trimeric form of SV-IV. The longhelices predicted...

Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

... study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also tostudy the effect of thermal unfolding in the presence and the absence of MgATP. ... esti-mation of the bandwidth and the intensity of the bandsmaking up the amide I or I¢ region. Thereafter, curve ﬁttingwas performed and the heights, widths and positions of each band were optimized ... secondary structure of the catalyticdomain of PKCf, its denaturation properties and the effect of its substrate, MgATP, on the structure and denaturation properties. Comparison of the structurein...

Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

... Zn2+.Furthermore, the d etermination of the Nogo-40 solutionstructure offers a starting point for further understanding the interaction between NgR and Nogo-40, and for future designs of molecules ... clue for future functional studies of Nog o-A.On the other hand, the recent identiﬁcation of Nogo-40as a potent NgR antagonist suggests a promising startingpoint for the design of potential therapeutic ... conformation for the p eptide.NMR s pectroscopy was further utilized to explore the structural properties of Nogo-40. The very narrow reson-ance dispersion of amide protons ( 0.7 p.p.m) and the lackof...

Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx

... and 2-amino-2,6-dideoxygalactose, the 1H-NMR spectra of which were identical with those of authentic referenceglycoses, and further conﬁrms their characterization. The combined MS data and the isolation of the two aminoglyc-oses ... spectra and the characterization of the glycosecomponents in the O-PS were determined from the appli-cation of COSY, TOCSY, NOESY and 1H,13C-HSQC and HMBC experiments (Table 1, Fig. 2). For the ... and J1,28.8 Hz. Thecorrelation of H2C tothe C2C at55.4 p.p.m.is consistent with the presence of a C2 acetamido substituent, and the lack of a proton at C6, considered in conjunctionwith the...

Báo cáo khoa học: Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin potx

... oligomersBecause of the large size of the core of the oligomer, the NMR spectra do not show any signals from the interior of the core and thus do not give any informa-tion on its structural details. Nevertheless, ... structural and dynamic characteristics of the individual domains has the potential to throw valuable light on the interac-tions of the individual domains, and the mechanism and variety of the overall ... packing of the helicesin the core of the oligomer. Because GED is the pri-mary driver of dynamin assembly, all these observa-tions would throw valuable light on the extent and mechanism of the...

Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx

... repeating units.Fig. 4. CE-ES-MS analysis (+ ion mode) of LPS (A) and carboxyl-reduced LPS (B) of A. salmonicida. The inset sho ws the ratio of the native and amidated forms of LPS and CPS. Separation ... 2-acetamido-2-deoxy-D-galacturonic acid. The sequence of constituent glycoses and the location of L-Alawere conﬁrmed by 2D NMR analysis performed on bothCPS and aqueous-phase LPS and their methanolysisproducts, and CE-ES-MS ... stainless steel needle (27 g) w as buttedagainst the low dead volume tee and enabled the delivery of the sheath solution t o the end of the capillary column. The separations were obtained o n  90-cm...

Báo cáo khoa học: Structural determination of the polar glycoglycerolipids from thermophilic bacteria Meiothermus taiwanensis pdf

... essential for the thermal stability a ndbiological functions of the bacteria i n extreme environments[9–11]. The polar lipids found in Thermus aquaticus,Thermus filiformis, Thermus scotoductus,andThermus ... study is the first to determine the full structure of glycoglycerolipidin thermophilic bacteria Meiothermus spp. The structural information will be very useful for further investigations of the mechanisms ... strains of thermophilic eubacterialgenus T. aquaticus and T. filiformis [15].Glycosyl linkage and anomeric configurationWith the solid results of the sugar composition and sequence, the linkages and...

Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identiﬁcation of sulfated high-mannose type oligosaccharides doc

... 6B).Altogether, these data conﬁrm the presence of sul-fated high-mannose type oligosaccharides in cruzipain and in its C-terminal domain. This is the ﬁrst report of the use of nor-harmane as matrix for ... or from the whole protein) and usingdifferent equipment. Therefore, the retention of watercan be explained taking into account its strong interac-tion with the negative charge site of the sulfated ... Likewise, these glycopro-teins are localized in lysosome, however, Man6-SO4accounts for the majority of the sulfated sugar.In conclusion, the results obtained provide evidence of the nature of the...

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