Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot

... FEBS Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental ... full-length cDNA of PO -CHH and identified the presence of the neuropeptide forms in the ES and PO. For the first time in crustaceans, the expression profiles of these two CHHs in response to oxygen and tempera-ture ... winter and anoxia during summer, in combination with the changes in salinity, are associated with high mortality in this species [2 5–2 7]. In view of CHH involvement in response to stress in other crustacean...

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... phosphorylation to the corresponding triphosphate nucleoside, converting the pro-drug to the active cytotoxic drug. The nucleo-side analogue can be incorporated into the DNA chain and cause chain termination, ... hydro-gen bonds with the base, was mutated to N in order to see how the increased distance between the base and substrate-binding amino acid affected the binding of dThd and deoxycytidine (dCyd).ResultsWe ... alignment of wild-type Dm-dNK (blue) (PDB ID:1OE0) and Dm -dNK-E52D (red) illustrating the binding of the feed-back inhibitor dTTP and the position of Mg2+ in the active site.L. Egeblad-Welin et...

Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

... regulatory TnC-binding site,from actin, resulting in the release of the inhibition and muscle contraction [17]. The structural TnC-bind-ing site interacts with the C-domain of TnC in both the ... TnC-binding site and the C-domain of TnC accompanyingCa2+binding to site IV of TnC. In vertebrate tropo-nin, the activation may be a result of the interactionbetween the inhibitory region and ... that the C-terminal region of TnI (including the inhibitory region and the regula-tory TnC-binding site) exhibits a positional changefrom actin-tropomyosin ﬁlament to the N-domain of TnC in a...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... sulfonamide [R–S(O2)–NH–R¢] [23], sulfamate [R–O–S(O2)–NH–R¢] [24], sulfamide[R–NH–S(O2)–NH–R¢] [25,26], and N -acylsulfamate[R–O–S(O2)–NH–C(O)–R¢] [27]. Of these functional groups, only the ... the Kmvalues is attributable to the uracil moiety binding in the same fashion [38], which could trigger the initialbinding of both substrates. In UpG, the binding of the guanine moiety is deterred ... resonance studies of the structure and binding sites of enzymes. 8. Inhibitor binding to ribonucleases. J Mol Biol 45, 49 1–5 11.33 Ui N (1971) Isoelectric points and conformation of proteins. I....

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... that has the IMPACT tag fused to the authenticC-terminal Gly145. Expression and puriﬁcationRecombinant protein was produced by cultivating the expression strain in shaking ﬂasks containing LB ... byxanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto-chrome c being reduced by superoxide in the course of the reaction. The principle of ... 32 2–3 24.33 Fay DP, Nichols AR & Sutin N (1971) The kinetics and mechanisms of the reactions of iron(III) with b-dik-etones. The formation of monoacetylacetoatoiron(III) and the effect of...

Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx

... seems to have on the RNase activity of the enzymes may be linked to an increase in Na+around the phosphate groups and the 2¢-OH, whichreduces the negative charge, and hence the afﬁnity of the enzyme ... N-terminus (Thr and Met) are encoded by the expression vector.An analysis of the amino-acid composition showsthat VsEndA contains 13 more lysines and two fewerarginines than Vc EndA, resulting in ... alignment showing the amino acids of VsEndA and Vc EndA. Numbers indicate cysteines involved in disulﬁde bridges; starsindicate Mg2+-co-ordinating residues, triangles indicate the catalytically...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... DEC1 with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for ... 5 ). These r esults indicate that the bHLHregion, including His57 and Arg65, is responsible for t heE-box binding.Determination of the region in BMAL1 for binding to DEC1 To identify the region ... productswere cloned into pCR-Blunt II-TOPO (Invitrogen). The cDNAs thus obtained were conﬁrmed by nucleotidesequencing and then subcloned into the expression vectorpcDNA3.1/Zeo (Invitrogen) or the mammalian...

Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in ﬁbrinolysis and plasmin-induced cell detachment and apoptosis docx

... Ponting CP (1998) Evidence that the conformation of unliganded human plasminogen ismaintained via an intramolecular interaction between the lysine-binding site of kringle 5 and the N-terminalpeptide. ... [35] and have used them to analyse, in functional isolation, the role of these LBS on plas-min(ogen) binding and activation. The effect of thesemAbs on plasmin formation and function was furtheranalysed ... The inhibitory effect of the mAbs on plasminogen binding to ﬁbrin and cells isexpressed as a percentage (mean ± SD, n ¼ 3) relative to the amount of plasminogen bound in the absence of mAbs. The...

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... destabilize the proteins leading to spurious protein–protein or /and protein–DNA interactions. The crystal structure of Ydc2 shows that deletion of the twoN-terminal helices 1 and 2 would expose to the ... base-pairs in the centre of the junctions [19]. Detailedunderstanding of the functional role of these interactionswould require the crystal structure of the Ydc2–junctioncomplex. In this study the ... conformation of the junction, then the deletion of this motif would have aneven more serious effect on Ydc2 binding in the presence of divalent cations. We tested the effect of Mg2+on the binding of the...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... preventing the release of EF-Tu–GDP from the ribosome [24]. Theseantibiotics bind at the interface between domain 1 (guaninenucleotide-binding domain, G-domain) and domain 3 of EF-Tu, and lock EF-Tu–GDP ... translationindirectly by inhibition of mRNA synthesis during aminoacid starvation [2 0–2 3].Kirromycin is one in a family of antibiotics that inhibitsprotein synthesis by binding EF-Tu–GTP and preventing the ... domain; and the C-terminal module [9]. The dimerization domain(residues 18 0–2 28), which consists of a-helices 9, 10 and 11,is inserted in subdomain C of the core domain and contains the protruding...

Xem thêm