Báo cáo khoa học: Effects of the G376E and G157D mutations on the stability of yeast enolase – a model for human muscle enolase deficiency pdf
Báo cáo khoa học: Effects of the G376E and G157D mutations on the stability of yeast enolase – a model for human muscle enolase deficiency pdf
... Journal 275 (2008) 9 7–1 06 ª 2007 The Authors Journal compilation ª 2007 FEBS Effects of the G376E and G157D mutations on the stability of yeast enolase – a model for human muscle enolase deficiency Songping ... believe that the effects of these mutations on the structure and function of yeast enolase will be similar to their effects on human bb -enolase. Figure 1 shows the basic fold of yeast enolase, including ... position would result in a change in the conformation of this loop. Changes in the conformation of the backbone at this point and changes in the orientation of other side chains, as a result of the...
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Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant pdf
... refinement at the CNSrefinementstage. A random sample of 10% of the X-ray data wasexcluded from the refinement and was taken as the test dataset, and the agreement between the calculated and observedstructure ... proteinatoms. The former propionate displays the conservedconformation in the structures of the Phe35fiTyr mutant and of the wild-type protein as well as other mutants.However, the conformation of the latter ... described mathematically by the equationDAt¼ DAeq(1–e–kt)whereDAtis the increase in absorbanceat time t, DAeqis the increase in absorbance at equilibrium, and k is the rate constants for...
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Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc
... Biotech, Auburn, CA, USA). All researchmice at CINVESTAV and Trudeau Institute were eutha-nized by CO2narcosis in accordance with the recommenda-tions of the Panel on Euthanasia of the AVMA and ... dependent on the presence of stable homodimersAs the mutations in the cytoplasmic region and the a1 -helix of CD38 affected dimer stability upon solubilization,plasma membrane expression levels and ... however, the structuralrequirements for their stabilization on the plasma mem-brane are unknown. We demonstrate that the majority of CD38 is assembled as noncovalently associated homo-dimers on the...
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Báo cáo khoa học: Nuclear factor kappa B and tumor necrosis factor-alpha modulation of transcription of the mouse testis- and pre-implantation development-specific Rnf33⁄Trim60 gene pot
... (5¢-GCCTCCTGCACCACCAACTG-3¢) and GAPDH-R (5¢-CCAGTAGAGGCAGGGATGATGT-3¢). The real-time PCR program was: pre-incubation at 50 °C for 2 min; initial denaturation at 95 °C for 7 min; and 45 cyclesat 95 °C for ... used for statistical analysis of the luciferase assay data; values of P < 0.05 were considered significant.Preparation of nuclear extracts and total proteinlysatesNuclear extracts and protein ... discerned(Fig. 3A; sequence on top). For analysis of their rolesin transcription, aTATA was deleted in constructF1DaT and Inr was mutated in construct F1mutI; both the aTATA deletion and the Inr mutation...
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Tài liệu Báo cáo khoa học: FGF-2, IL-1b and TGF-b regulate fibroblast expression of S100A8 doc
... Bio-Rad) and analyzed withMultianalyst 1.0 software (Bio-Rad). Alternatively, the FLA5000 imaging system (FUJI) and the L Process and Image Gauge software packages were used. The relat-ive magnitude ... Y, Miyazaki M, NambaM, Yamada H & Tanaka T (2001) Loss of nuclear loca-lization of the S100C protein in immortalized human fibroblasts. Radiat Res 155, 20 8–2 14.12 Yang Z, Tao T, Raftery ... transforming growth factor-b1, and basic fibroblast growth factor in dermal woundhealing: neovessel and matrix formation and cessation of repair. Am J Pathol 140, 137 5–1 388.56 Zhang HY, Gharaee-Kermani...
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Báo cáo khoa học: Functional interplay between viral and cellular SR proteins in control of post-transcriptional gene regulation pptx
... regulation of alternative splicing, and also controls their subnuclearlocalization and nucleocytoplasmic transport [ 1–6 ].Moreover, environmental signals or viral infection cancontrol the phosphorylation ... viral DNA replication [16]. The E2 protein consists of the N-terminal transacti-vation domain and the C-terminal DNA-bindingdomain. These two functional domains are linked by a hinge region ... proteins are important for regulation of viral RNA splicing and participate in other steps of post-transcriptionalviral gene expression control. Moreover, viral infection may alter the expression...
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Báo cáo khoa học: Unchanged thymidine triphosphate pools and thymidine metabolism in two lines of thymidine kinase 2-mutated fibroblasts docx
... and presented contrasting and dis-tinct features of myopathic damage. Although the overall condition of the patients deteriorated withtime, in both cases an apparent negative selection of abnormal ... Chiara Rampazzo1, Elisa Franzolin1, Mari-Carmen Lara2, Maya R. Vila`2,Ramon Martı´2 and Vera Bianchi11 Department of Biology, University of Padova, Italy2 Institut de Recerca Hospital ... pool had reached a specific radioactivity of about 7000 c.p.m.Æpmol)1in Ca and Pb cells, and about 5000 in Cb and Pa cells (Fig. 2B), indicatingthat one-third of the dTTP of Ca and Pb cells and between...
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Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... (forward, 5¢-TCAGCATTGCAAATAACAATCTCA-3¢; reverse, 5¢-CAGTCTGCTCTTTGTTGCACATATAA-3¢), CTGF (forward, 5¢-CAAGCTGCCCGGGAAAT-3¢; reverse, 5¢-GGACCAGGCAGTTGGCTCTA-3¢), JUN (forward, 5¢-GGATCAAGGCGGAGAGGAA-3¢;reverse,5¢-TCCAGCCGGGCGATT-3¢),PLAU ... ⁄siRNA2, LAMP2 ⁄ siRNA1 and LAMP2 ⁄ siRNA2 are 5¢-UGACUUCCCUGGCCUAUUUUU-3¢,5¢-ACAUUGAGCUCCUCUCUUGUU-3¢,5¢-GAUAAGGUUGCUUCAGUUAUU-3¢ and 5¢-ACAGUACGCUAUGAAACUAUU-3¢,respectively. As a negative ... reverse, 5¢-GGAAGGAGTGGATTTAGATCACAAGA-3¢), TGFBR2 (forward, 5¢-TGGACCCTACTCTGTCTGTGGAT-3¢; reverse, 5¢-TTCTGGAGCCATGTATCTTGCA-3¢) and TBP ⁄ TFIID (forward,5¢-CCTCACAGGTCAAAGGTTTACAGTAC-3¢; reverse,5¢-GCTGAGGTTGCAGGAATTGAA-3¢)...
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Báo cáo khoa học: Effects of salt on the kinetics and thermodynamic stability of endonuclease I from Vibrio salmonicida and Vibrio cholerae potx
... production and thermal stability The VsEndA variants Q69N and K71N and the VcEndA variants N69Q and N71K were expressed in a soluble form at levels comparable with the nativeendonucleases. The mutations ... Tokunaga H, Arakawa T, Fukada H & Tokunaga M(2006) Opposing effects of NaCl on reversibility and thermal stability of halophilic beta-lactamase from a moderate halophile, Chromohalobacter ... image and 0.5° oscillation, and a total of 78° of data were used in the final data set. The data were integrated with the programmosflm [44], scaled with scala, and the structure factorsobtained...
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Báo cáo khoa học: Effects of cardiomyopathic mutations on the biochemical and biophysical properties of the human a-tropomyosin docx
... pastoris,toinvestigate the effects o f cardiomyopathic-related m utations on the human skeletal Tm. Our data indicate that the main effects of mutations A6 3V, K70T and E180G are mainlyrelated to the overall stability ... (5¢-GACAAATACTCTGAAGTACTCAAAGATGCCCAG-3¢), SKTM-1R (5¢-CTGGGCATCTTTGAGTACTTCAGAGTATTGTC-3¢), SKTM-K70T (5¢-AAAGATGCACAGGAGACGCTGGAGCTGGCAGAG-3¢), SKTM-2R (5¢- CTCTGCCAGCTCCAGCGTCTCCTGTGCATCTTT-3¢), ... Tmsmigrated with an apparent molecular mass of 36 kDa and slightly slower migration was observed for the mutantK70T. Mutations A6 3V and K70T are located at the N-terminal region of the protein and...
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