Báo cáo khoa học: Small heat shock protein Hsp27 prevents heat-induced aggregation of F-actin by forming soluble complexes with denatured actin docx

... Authors Journal compilation ª 2007 FEBS Small heat shock protein Hsp27 prevents heat- induced aggregation of F -actin by forming soluble complexes with denatured actin Anastasia V. Pivovarova1,2, ... stable and highly soluble complexes with denatured actin, and this is the mecha-nism by which sHSPs prevent the aggregation of F -actin. Stable complexes of small HSP with denatured actin A. V. Pivovarova ... Biophys Res Commun 331, 1548–1553],and supposed that Hsp27 prevents heat- induced aggregation of F -actin by forming soluble complexes with denatured actin. In the present work, weapplied dynamic light...

Báo cáo khoa học: The heat shock protein 70 molecular chaperone network in the pancreatic endoplasmic reticulum ) a quantitative approach potx

... [21].Quantitation of proteins in dog pancreasmicrosomesThe amount of protein present in the band of a gel of aGST hybrid preparation was determined by comparison with protein standards that ... 6A,B). Inthe absence of ATP, a proportion of BiP cofractionat-ed with Grp170, with an elution maximum of bothproteins at a position that corresponded to a molecularmass of 240 kDa (Fig. 6A). ... cochaperones of the ER in the canine pancreas. The concentrations refer to a suspension of RMs, with a concentration of heterotrimeric Sec61 complexes of 2.12 lM [54]. The afﬁnities of Hsp40s for...

Tài liệu Báo cáo khoa học: The heat shock factor family and adaptation to proteotoxic stress pdf

... 2010)doi:10.1111/j.1742-4658.2010.07827.xThe heat shock response was originally characterized as the induction of aset of major heat shock proteins encoded by heat shock genes. Because heat shock proteins act as molecular ... eukaryotes,the heat shock response is regulated mainly at the level of transcription by heat shock factors (HSFs) [3]. Heat shock genes, such as HSP110, HSP90, HSP70, HSP40and HSP27, contain heat shock ... expression of certain nonclassical heat shock genes. Thus, the heat shock response regulated by the HSF family should consist of theinduction of classical as well as of nonclassical heat shock genes,...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... activated by members of the Ras family of small G proteins.The activity of Ras proteins is under tight control of several classes of guanine nucleotide exchange factors(GEFs) and GTPase-activating proteins. ... prior to 5 min of treatment with 10 lm serotonin (Fig. 6B). Increasing the free intracel-lular levels of Ca2+ by treatment of HEK293 cells with thapsigargin induced phosphorylation of ERK1 ⁄ 2(Fig. ... transfected with the 5-HT7(a)receptor,were treated with or without 40 lM BAPTA-AM for 25 min prior toincubation for 5 min with or without 10 lM serotonin. (C) and (D)HEK293 cells were treated with...

Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

... family of proteins that bind DNA at unmethylated CpGislands [7], which are always located to active chroma-tin. Other proteins identiﬁed are fungal homologues, of which suppressor of PRP protein ... termSPP1. All these proteins contain a small, previouslyundetected domain downstream of the PHD, whichwe called dPHD, that allows connection of these famil-ies (despite the small length of alignment) ... of proteins associated with a trithorax-related SET domain protein. Proc NatlAcad Sci USA 98, 12902–12907.9 Tenney K & Shilatifard A (2005) A COMPASS inthe voyage of deﬁning the role of...

Báo cáo khoa học: Small peptides derived from the Lys active fragment of the mung bean trypsin inhibitor are fully active against trypsin pptx

... Chi1,21 Institute of Protein Research, Tongji University, Shanghai, China2 Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, ChinaIntroductionProteinase inhibitors ... fragments of MBTI were successfully separated by restricted pepticdigestion [23], and the sequence of MBTI was eluci-dated [24]. The active fragment with Arg at the reac-tive site P1is composed of ... from the long chain, with three intramolecular disulﬁde bonds, was synthesized,giving two disulﬁde isoforms, both of which remainedactive against trypsin, with Kivalues of 1.2 · 10)7mand...

Báo cáo khoa học: Fragile X-related protein FXR1 controls posttranscriptional suppression of lipopolysaccharide-induced tumour necrosis factor-a production by transforming growth factor-b1 doc

... overexpression of FXR1 suppresses LPS-induced TNF-a protein production. Targeting the p38 mitogen-activated protein kinase pathway of LPS-treated cells with small molecule inhibitorscan induce FXR1 protein ... obtained by measure-ment of the TNF-a protein concentration by ELISA(Fig. S1A–C).To determine whether TGF-b1-dependent inhibition of LPS-induced TNF-a protein production occurred atthe level of ... regulated by TGF-b1.Induction of FXR1 expression by TGF-b1 leads topost-transcriptional downregulation of TNF-a protein Many RNA-binding proteins, such as TTP, TIA-1,HuA, TIA-1-related protein, ...

Tài liệu Báo cáo khoa học: An engineered disulﬁde bridge mimics the effect of calcium to protect neutral protease against local unfolding docx

... topermit quantiﬁcation of protein degradation at protein concentrations of < 20 lgÆmL)1, autoproteolysis atlower protein concentrations was followed by measure-ment of the remaining activity ... Autoproteolysis wasquantiﬁed by evaluation of the bands of intact protein in SDS ⁄ PAGE gels after staining with Coomassie brilli-ant blue. Figure 2 shows the autoproteolysis of pWT at5.5 m GdnHCl. ... deceleration of the unfoldingprocess by almost six orders of magnitude after bind-ing of all four calcium ions, corresponding to anincrease in Gibbs free energy of activation of unfold-ing,...

Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity pdf

... phosphorylation by Akt (Eur. J. Biochem. 271) 3335Phosphorylation of dHAND did not affect thedimerization activity with E47To clarify the mechanisms of the inhibition of dHANDactivity by Akt phosphorylation, ... activity of dHAND could beaffected by Akt phosphorylation. The inﬂuence of phos-phorylation by Akt on the DNA binding activity wasexamined by gel shift analysis. We included the E -protein Fig. ... ctivity w ithE -protein, (b) DNA binding activity of dHAND/E -protein, and (c) transcriptional a ctivity of dHAND/E -protein heterodimer. Our results indicated that the effect of Akt-dependent...

Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx

... (2002)Structure and properties of the small heat shock pro-teins (sHsp) and their interaction with cytoskeletonproteins. Biochemistry (Mosc) 67 , 511–519.5 Macario AJL (1995) Heat- shock proteins and molecularchaperones: ... interaction of a-crystallin with unfoldedproteins. Biochim Biophys Acta 1252, 251–260.23 Bova MP, Mchaourab HS, Han Y & Fung BK-K(2000) Subunit exchange of small heat shock proteins:analysis of ... and expression of the small heat shock proteins hsp27 and aB-crystallin in Parkinson’sdisease. NeuroReport 10, 2273–2276.10 Head MW & Goldman JE (2000) Small heat shock pro-teins, the...

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