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Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf

Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf

Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf

... conclusion that lactate produc-tion in strain FI9078 was mediated by the enzyme encoded by the ldhB gene. As expected, the enzyme pro-duced by strain MG1363 was encoded by the ldh gene. LDHB (ldhB gene ... 50% of the maximum rate; LDH, L -lactate dehydrogenase encoded by the ldh gene; LDHB, L -lactate dehydrogenase encoded by the ldhB gene; LDH- Bs, lactate dehydrogenases of Bacillus stearothermophilus; ... of ldhB. The biochemical and kinetic properties of this alternative lactate dehydrogenase (LDHB) were compared to those of the ldh -encoded enzyme (LDH) , puri-fied from the parental strain. In...
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Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... plasmidcombinations were spotted either onto medium lacking histidine and leucine to select for the plasmids, or onto medium lacking histi-dine, leucine and uracil to monitor protein interactions. The ... acyltransferase wasdetermined in an are1 deletion background. The enzyme activity in the ste20D strain and the cla4D strainwas indistinguishable from that in the wild-type strain(Fig. 7A). Therefore, it ... dele-tion of either ERG4 or NCP1 in the cla4D backgroundis lethal, indicating that CLA4 and these genes, encod-ing proteins involved in sterol synthesis, may function in the same pathway(s) [25]....
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Báo cáo khoa học: A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus potx

Báo cáo khoa học: A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus potx

... RI binding. The affinity of porcine ribonuclease inhibitor (pRI) for the 1C zymogen was determined by using a competitivebinding assay with fluorescein-labeled G88R RNase A[36]. The resulting ... v), and dithiothreitol (0.005 m) [48]}, and incubating the resultingmixture at 37 °C for 15 min. Activation was stopped by dilution (1 : > 1 0) into 0.10 m Mes ⁄ NaOH buffer, pH 6.0,containing ... added to 2.0 mL NaCl ⁄ Picontaining dithiothreitol (5 mm), and the resulting solution was incubated at 23 (± 2) °C for 20 min.After this incubation, the initial fluorescence intensity ofthe...
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Báo cáo khoa học:

Báo cáo khoa học: "Impact of computerized physician order entry on medication prescription errors in the intensive care unit: a controlled cross-sectional trial"

... eval-uated by the clinical pharmacist, comprising 1,286 in the C-U and 1,224 in the PB-U. In the C-U, 44 MPEs occurred versus331 in the PB-U (3.4% versus 27.0%, P < 0.00 1). Overall, the ICIS ... inci-dence of minor MPEs decreased from 18.3% in the PB-U to 0.7% in the C-U, since completeness and legibility of the orderwas mandatory in the CPOE part. However, a missing infusionrate was ... purposes)many advanced features to prevent errors in the administrationprocess by showing important information to the nursing staffregarding administration procedures and safety.As in our...
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Báo cáo khoa học:

Báo cáo khoa học: "Circadian pattern of activation of the medical emergency team in a teaching hospita"

... nursing handover (red, up-pointing arrows), the beginning and end of the daily medical shift (green, down-pointing arrows), and periods of routine nursing observations (pink, shorter, up-pointing ... hours'(18:00–08:0 0) when wards are not staffed by parent unit doc-tors. In addition, there was a trend toward increased frequencyof activation of the MET service during these hours in the 5years following the ... calls during 58% of the day), with the peak timeof activity occurring between 20:00 and 20:30. These findingshave important implications for the frequency and method ofpatient monitoring, as...
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BÀI BÁO CÁO KHOA HỌC TẠI BẮC KINH ATCM 2009 THE SCIENTIFIC REPORT IN BEIJING - CHINA

BÀI BÁO CÁO KHOA HỌC TẠI BẮC KINH ATCM 2009 THE SCIENTIFIC REPORT IN BEIJING - CHINA

... 1.Solution : The number contains the end digit is digit 1 The number contains the end two digits are digit 1 The number contains the end three digits are digit 1 The number contains the end four ... Z, T) = 0 =”, Str4: Lbl 1: Input « Xo = », A: Input « Yo = », B: Input « Zo = », C: Input « To = », D: 1 → N: String  Equ(Str1, Y 1) : String  Equ(Str2, Y 2) : String  Equ(Str3, Y 3) : ... Find the number contains the first four digits are digit 1.Solution :We have ( we get the number 223 ) We try ( answer contains two digitare digit 1 ) . we do not choose the number 223The...
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Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... °C) for 4 weeks, and then transferred to a growth chamber with a tempera-ture regime of 22 °C in the day (16 °h) and 16 °C at night(8 h). The light intensity was 50 lmolÆm )2 Æs )1 , and the ... arereversibly interconnected. The rate of sucrose export to sink organs (rSuc fi Sinks ) was calculated as the difference between the spline-interpo-lated rates of net photosynthesis and of changes in the carbohydrate ... thisfunction. The flux rate of CO2into sucrose synthesis(rCO2!Suc ) was caclulated as the difference between the rateof net photosynthesis and that of net starch synthesis (unit:C6h )1 Æg )1 FW):rCO2!Suc¼...
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Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx

Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx

... killing. In addition to actin, other host cell proteins have beenshown to bind to the virulence factor YpkA, includingOTUB1 [13]. In order to investigate the role ofOTUB1 in Yersinia invasion of HEK293T ... susceptibility to YersiniainvasionYersinia virulence factors are injected into target hostcell molecules to manipulate signalling pathways dur-ing invasion in order to prevent uptake and killing. In addition ... between the GTPase-bindingcapacity of YpkA and OTUB1-mediated increase in susceptibility to infection (Fig. 6). The binding of smallGTPases has been reported to be independent of the kinase activity...
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Tài liệu Báo cáo khoa học: Post-translational modifications of the linker histone variants and their association with cell mechanisms docx

Tài liệu Báo cáo khoa học: Post-translational modifications of the linker histone variants and their association with cell mechanisms docx

... mechanism include,but are not limited to, acetylation (lysine), phosphoryla-tion (serine, threonine), methylation (lysine, arginine),sumoylation (lysine), and ubiquitination (lysine). Otherepigenetic ... possible to distin-guish the respective cases? In the first case, the celllines were specifically treated to put them into mitosis;this was not so in the second and third cases. How-ever, in two cases ... although there is a good degree of agree-ment about the overall parameters of the fibre formed by folding the zig-zagging chain of nucleosomes in inactive chromatin, the location of the linker histonein...
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Tài liệu Báo cáo khoa học: KCNE4 can co-associate with the IKs (KCNQ1–KCNE1) channel complex ppt

Tài liệu Báo cáo khoa học: KCNE4 can co-associate with the IKs (KCNQ1–KCNE1) channel complex ppt

... explain the suppressionof IKs by KCNE4.Next, we examined the ability of KCNE proteins to traffic to the plasma membrane in the presence ofKCNQ1. Total protein, non-biotinylated and biotiny-lated ... Health, Bethesda, MD, USA). The percentageof protein at the membrane was calculated by dividing the biotinylated fraction value by the total protein fractionvalue, and is reported as the mean ... Complete miniprotease inhibitor tablet), and werecentrifuged for 30 min at 4 °C. The supernatant fractionwas collected and an aliquot was retained as the total pro-tein fraction. The remaining supernatant...
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