... exponential phase, and then incubated for 30 min in galac-tose medium to activate the GAL regulon. Total mRNAs wereextracted and subjected to standard northern blot analysis. GAL1mRNA, and ACT1 ... the S13 4A- S ⁄ S13 4A- AS, S13 3A- S ⁄ S13 3A- AS, S13 3A- S13 4A- S ⁄ S13 3A- S13 4A- AS and S134D-S ⁄ S134D-AS pairs of primers (Table S2). Transformation of yeast cells withrecombinant plasmids was performed as ... assayTo identify the interaction between Bud32 and Sch9, a pull-down analysis was carried out using the NiNTAaffinity matrix (Ni2+–nitrilotriacetic acid agarose) (Qiagen,Valencia, CA, USA)...