Báo cáo khoa học: Puriﬁcation and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus docx

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... & KwonYM (2001) cDNA cloning of two isoforms of ornithinecarbamoyltransferase from Canavalia lineata leaves and the effect of site-directed mutagenesis of the carbamoylphosphate binding ... This paper reports the puriﬁcation and characterization of GAT from wildwatermelon leaves, and discusses its function duringdrought ⁄ strong-light stress on the basis of its twounique enzymatic ... two each of the a and b subunits.Analysis of the N-terminal transit peptide of CLGATAnalysis of the CLGAT cDNA predicted a chloroplasttransit peptide upstream from the N-terminus of CLGAT....

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... sialidasetypeX ,protease enzymes and molecular mass standards were purchased from Sigma.Preparation of crab seraFreshwater field crabs, Paratelphusa jacquemontii werecollected from the local wetlands of ... Sarris and Palade [30] and Schauer [6]. Asolution of 750 lL of glycoprotein (5 mgÆmL)1) was addedto 250 lLof0.04M of NaOH, vortexed, incubated on icefor 45 min and neutralized with 1 mL of ... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crabParatelphusa jacquemontiiMaghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S....

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... complex from A. fulgidus (Eur. J. Biochem. 269) 1899absorption maxima at 420 nm (c band), 530 nm (b band) and 557 nm (a band) are characteristic of cytochrome b.Heme was extracted from the ... FEBS Lett. 457 , 291–297.1904 G. J. Mander et al.(Eur. J. Biochem. 269) Ó FEBS 2002 Purification and characterization of a membrane-bound enzymecomplex from the sulfate-reducing archaeonArchaeoglobus ... [2].Hdr from Methanothermobacter marburgensis is aniron-sulfur flavoprotein composed of the subunits HdrA,HdrB, and HdrC. The enzyme has been purified from thesoluble fraction, and none of its...

Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... min, bands withmolecular masses of 43 and 39 kDa were observed inaddition to undigested bands of ZP2 and ZP3. Afterincubation for 10 min, three major bands with molecu-lar masses of 43, 39 and ... to Professor F. S.Howell (Department of Materials and Life Sciences,Faculty of Science and Technology, Sophia University,Tokyo) for reading the manuscript and to DrK. Yamagami (former Professor ... and swellsthe envelope. The cross-species digestion using enzymes and substrates of zebraﬁsh and medaka revealed that both ZHE1 and MHCE cleaved thesame sites of the egg envelope proteins of...

Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... Isolation and charac-terization of the cysteine- proteinases from the latex of Carica candamarcensis Hook. Biol Chem Hoppe-Seyler374, 501–506.18 Walraevens V, Vandermeers-Piret M, Vandermeers ... precursors and areactivated in response to wounding of the plant [26].Similar to other known cysteine proteinases from Car-icaceae, the deduced protein sequences of VXH-A, -B,-C and -D, and VS-A and ... Fractionation and puriﬁcation of the enzymes stored in the latex of Carica papaya.J Chrom B 790, 229–238.32 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of the head of bacteriophage...

Báo cáo khoa học: Puriﬁcation and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus docx

... Authors Journal compilation ª 2008 FEBS 5443 Purification and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus Akihiko Tsuji, Yayoi Kikuchi, Kentaro ... BSA as a standard [40]. Purification of cathepsin B-like cysteine protease from daikon radishAll purification procedures were performed at 4 °C unlessotherwise indicated. Cotyledons of daikon radish ... of daikon radish (R. sativus) grown by hydro-ponics were purchased from a grocery store and used for purification of the enzyme. For analysis of expression of theenzyme during germination, daikon...

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. It ... 1977effect of the d-Phe on structure, was also studied. Theunusual structure of conomarphin further demon-strates the high diversity of conotoxins.Results Puriﬁcation and primary sequence of conomarphinAs ... structure of conomarphinwas similar to those of other conotoxin cDNAs. ThecDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 resi-dues, a proregion of...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... expression of T-bet has beenseen within a wide variety of tissues and cells in theGinbuna crucian carp [27] and, in mammals, in thelung tissue of mouse and spleen and thymus of human and mouse ... the expression of these genes within humans and mice, of T-betwithin Ginbuna crucian carp [27] and STAT6 inmandarin fish [28] have shown similar widespreadexpression. In the case of STAT6, highest ... of zebrafish t-bet, stat6 and foxp3 were also determined and found to be very simi-lar to their human homologues. Both the human and zebrafish T-bet and Foxp3 genes contained the samenumber of...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... properties of SmSmad1 and SmSmad1B asdescribed above. However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating theformation of ... Drosophila MAD and the vertebrate Smad1,Fig. 1. Structure of the SmSmad1B gene, cDNA and protein. (A) Schematic representations of the SmSmad1B gene, cDNA and protein and the amino acid sequence of SmSmad1B ... Fantappie2,Francisco M. B. Oliveira2 and Philip T. LoVerde1,21 Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, State University of New York, NY, USA2 Southwest...

Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... Purification and cDNA cloning of a cellulase from abaloneHaliotis discus hannaiKen-ichi Suzuki, Takao Ojima and Kiyoyoshi NishitaLaboratory of Biochemistry and Biotechnology, ... TCCAAACAGTCAGTTTCTTAACCGTTable 1. Purification of cellulase from abalone Haliotis discus hannai. Oneunitofcellulasewasdefinedastheamountofenzymethatliberatesreducing sugars equivalent to 1.0 lmol of glucose per min. Purification ... reverse primers R1 and R2 weresynthesized on the basis of sequences of LP5 and LP6,respectively (Table 2).PCR amplification of HdEG66-cDNAcDNAs encoding the N-terminal region of the HdEG66were...

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