Báo cáo khoa học: Pituitary adenylate cyclase-activating polypeptide attenuates streptozotocin-induced apoptotic death of RIN-m5F cells through regulation of Bcl-2 family protein mRNA expression pdf

... 2008 FEBS 5551 Pituitary adenylate cyclase-activating polypeptide attenuates streptozotocin-induced apoptotic death of RIN-m5F cells through regulation of Bcl-2 family protein mRNA expression Satomi ... STZ-induced apoptotic death of RIN-m5F cells through the regula-tion of pro -apoptotic proteins. These novel ﬁndings,taken together with previous observations, suggest thatthe anti -apoptotic effects of ... suppress the expression of Noxa mRNA and enhance Bcl-2 mRNA expression, leading to down -regulation of Bax mRNA. Thus,PACAPs appear to be a potent regulatory element inthe STZ-evoked apoptotic...

Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx

... common cause of death from can-cer among Mexican women [21]. Therefore, the aim of this work was to evaluate the effect of pharmacologicaldoses of DHEA on the proliferation and death of three cell ... suggest that the effect of DHEA upon CASKI cells is more cytotoxic thancytostatic.DHEA induces apoptotic and necrotic death To determine the type of death induced by DHEA, cells were analyzed for ... induce apoptotic cell death. Cisplatin andDHEA treatments resulted in apoptosis of bothHPV-positive cells and C33A cells, in comparison withuntreated cells (Fig. 5). The morphology of CASKIand...

Báo cáo khoa học: "Medical treatment for the terminally ill: the ‘risk of unacceptable badness’"

... terminally ill: the ‘risk of unacceptablebadness’David CrippenAssociate Professor, Director, Neurovascular ICU, Department of Critical Care Medicine, University of Pittsburgh Medical Center, ... maintaining some of the vitalfunctions of the body, but the same technology does notnecessarily allow us to heal underlying disease processes [2].An unintended side effect of modern technological ... plausibility of maintaining moribund patients in astate of suspended animation for prolonged and sometimesindefinite periods [3]. Also, advanced resuscitationtechniques make it possible to convert death...

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... sus-ceptible cells at multiple levels [62]. In resting humanCD4+T cells, the expression of ITK is extremely lowand often undetectable in immunoblot analysis. Theactivation of CD4+ cells, however, ... absence of B lymphocytesand plasma cells and very low levels of immunoglobu-lins of all classes. Humoral immune responses areessentially nonexistent. T cells are not affected, butmyeloid cells ... fusion proteins lead to differential phosphorylation levels of the fusion proteins themselves, as well as the downstream adapterproteins SLP76 and BLNK, in 293T and COS7 cells. Size of redencircled...

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... systemsthere is compelling evidence for the control of energymetabolism via the central nervous system (CNS),notably through the regulation of feeding behaviorand satiety [5,6]. Furthermore, ... entry of fatty acid into mitochon-dria. Thus, the steady-state level of malonyl-CoA inthese tissues is determined by the relative activities of ACC and malonyl-CoA decarboxylase.Role of the ... aspects of the affects of fructose on foodintake, these ﬁndings lend further support to the mech-anism by which malonyl-CoA participates in the regu-lation of feeding behavior.Role of carnitine...

Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... abundant proapoptotic protein of the Bcl-2 family that is crucial for death receptor-mediatedapoptosis in many cell systems [16,17]. The BH3domain-only proteins are a subfamily of the Bcl-2 family ... activity after the treatment of cells with TRAILor TRAIL and EGF in control cells, cells expressingGFP–Itch or cells with reduced Itch expression (Fig. 3B). The treatment of cells with EGF signiﬁ-cantly ... thusprotect cells from TRAIL-induced apoptosis.The antiapoptotic effect of EGF stimulationdepends in part on the function of ItchTreatment of cells with EGF has been variouslyreported to protect cells...

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... the membrane localization of proteins will facilitate the formation of protein com-plexes and increase reaction rates [35]. To mimic thein vivo situation, Src and other proteins can be embed-ded ... deregulation of Src signaling in cancer. A central result of our studyreveals that all necessary prerequisites for the diverse,baroque dynamics of Src responses already exist in theabsence of ... state of the cell can be verydifferent (e.g. with high or low activity of kinases andthe expression of particular genes). Instructively, excit-able systems can also display two distinct kinds of...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... (2001) Characterization of binding of leukotriene C4 by human multidrug resistance protein 1. Evidence of differential interactions with NH2-andCOOH-proximal halves of the protein. J Biol Chem276, ... increasing concentrations of an anticancer drug.The resulting resistant cells expressed high levels of ABC transporters compared to the minute expression of these proteins in parent lines. It ... a doubling of theamount of TMRM taken up into the cells. To charac-terize further the mechanism responsible for theexclusion of rhodamine dyes from cells, we surveyed avariety of agents known...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... common drawback of such approachesis the cost of the afﬁnity resins used to isolate thefusion protein and the proteases required to liberatethe protein of interest from the fusion protein. Suchconsiderations ... humanproteins smaller than about 50 residues is oftenhampered by proteolytic degradation of unstructuredproteins ⁄ peptides; therefore small entities are com-monly expressed fused to a larger protein ... broader coverage of struc-tural assignments to the N-terminus of Ab. Moreover,the procedures described here are also suitable for expression and puriﬁcation of mutant versions of Alzheimer’s...

Tài liệu Báo cáo khoa học: Marine toxins and the cytoskeleton: a new view of palytoxin toxicity ppt

... this respect, the outcome of ostreocin-D treatment of CaCo-2 cells remainsunknown.The impact of palytoxin action on actin filament sys-tem is not restricted to epithelial cells. In a recentreport, ... natural blocker of the Na+pump and an inhibitor of palytoxin action, have pro-vided interesting findings. Through the partial inhibition of Na+⁄ K+-ATPase, and regardless of changes in intra-cellular ... proposed that the ouabain-bound Na+⁄ K+-ATPase is capable of recruiting andactivating protein tyrosine kinases through specific protein protein interactions [45]. On the other hand, itshould be...

Xem thêm