Báo cáo khoa học: Fully active QAE isoform confers thermal hysteresis activity on a defective SP isoform of type III antifreeze protein docx
Báo cáo khoa học: Fully active QAE isoform confers thermal hysteresis activity on a defective SP isoform of type III antifreeze protein docx
... Fully active QAE isoform confers thermal hysteresis activity on a defective SP isoform of type III antifreeze protein Manabu Takamichi1,2, Yoshiyuki Nishimiya1, Ai Miura1and Sakae Tsuda1,21 ... that the ice growth inhibitory activity of an antifreeze protein isoform lacking the active component isrestored by the addition of a minute amount of the active isoform. AbbreviationsAFGP, antifreeze ... as an effective TH substance by the addition of a minute amount of the fully active antifreeze substance.Materials and methodsSample preparationRecombinant proteins of the type III AFP isoforms...
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Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf
... 88,12–19.19 Ohtani N, Yanagawa H, Tomita M & Itaya M (2004)Identification of the first archaeal type 1 RNase H genefrom Halobacterium sp. NRC-1: archaeal RNase HI cancleave an RNA-DNA junction. Biochem ... Type 1 RNase H [19,20]. Archaeal Type 1 RNase H can cleave an RNA–DNA junction (a junction between the 3¢ side of RNA and 5¢ side of DNA) of an Okazaki fragment-like substrate (RNA9–DNAÆDNA), unlike ... function as a phosphatase. Therefore, the phosphatase activity of the SCO2299 protein was examined by using p-nitro-phenyl phosphate (pNPP) as a substrate. When activity was assayed at various...
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Báo cáo khoa học: An active triple-catalytic hybrid enzyme engineered by linking cyclo-oxygenase isoform-1 to prostacyclin synthase that can constantly biosynthesize prostacyclin, the vascular protector pot
... Invitrogen (Carlsbad, CA, USA). [14C]AAwas purchased from Amersham (Piscataway, NJ, USA).Goat anti-(rabbit IgG)–fluorescein isothiocyanate (FITC)conjugate, saponin, SLO, Triton X-100 and triethylenedi-amine ... COX-2 has an inducible nature, has a lower capacity to be stably expressed, and may alsolead to numerous pathological processes, such ascancers and inflammation. Given the nature of COX-1, a housekeeping ... COX-1–10aa–PGIS protein than COX-2–10aa–PGIS protein under identical conditions (Fig. 2A, lane 2).Subcellular localization of COX-1–10aa–PGISTo determine whether the linkage of the C-terminalLeu of...
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Báo cáo khoa học: Biologically active, non membrane-anchored precursors – an overview ppt
... regulation of proliferation and sur-vival), Janus-activated kinase 2, signal transducer andactivator of transcription 3 (which is recognized as anoncogene implicated in many cancers) and extracellu-lar-signal ... nuclear localization in addition to itslocalization in the cytoplasm and was implicated in a transcription control role. In gene array assays, CgBinduced or suppressed transcription of many genes,including ... genes,including those of transcription factors [22].Assays for granins, especially CgA, are of great clin-ical use because circulating granins have served asdiagnostic markers for a variety of neuroendocrinetumors...
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Báo cáo khoa học: Functionally active fusion protein of the novel composite cytokine CLC/soluble CNTF receptor pdf
... 1B,left panel). SDS/PAGE analysis r evealed a single banddisplaying an ap parent m olecular weight o f 85 kDa, whichwas quantified based on known concentrations of BSA run on neighboring lanes. ... activity wasnormalized based on protein concentrations.FACS analysis and cytokine displacementBAF GLC and SK-N-GP cells were incubated in thepresence of increasing concentrations of putative ... 19, 5326–5338.56. Takahashi-Tezuka, M., Yoshida, Y., Fukada, T., Ohtani, T.,Yamanaka, Y., Nishida, K., Nakajima, K., Hibi, M. & Hirano, T.(1998) Gab1 acts as an adapter molecule linking...
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Báo cáo khoa học: Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase a is required for Src activation doc
... mutation had an influence on the catalytic activity of RPTPa-D2 in vitro by usingglutathione S-transferase (GST)-fusion proteins andpNPP as a substrate. The phosphatase activity of D2-R554H was ... indi-cate that the catalytic activity of RPTPa-D2 plays animportant role in Src activation.To confirm that the catalytic activity of RPTPa-D2 isrequired for Src binding and activation, we used anRPTPa-D2 ... compared with that of wild -type (WT)-D2 and with that of D2-C723S, a catalyticallydead mutant with a mutation in the essential catalyticsite cysteine. The catalytic activity of D2-R554H wasdramatically...
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Báo cáo khoa học: The b domain is required for Vps4p oligomerization into a functionally active ATPase potx
... 5¢-AGCACGTTAATCAATTGACTACGGTTGCATTAGCGC-3¢Vps4 Ter2 F 5¢-TTAAAAGAACCAGATTAGTCAATTGATTAACGTGCT-3¢Vps4 Ter2 R 5¢-AGCACGTTAATCAATTGACTAATCTGGTTCTTTTAA-3¢Vps4 RDE F 5¢-AAGCAAGAACAGTTCACTGCAGCTTTTGGTCAAGCAGGTAACTAGTCAATTGAT-3¢Vps4 ... 5¢-AAGCAAGAACAGTTCACTGCAGCTTTTGGTCAAGCAGGTAACTAGTCAATTGAT-3¢Vps4 RDE R 5¢-ATCAATTGACTAGTTACCTGCTTGACCAAAAGCTGCAGTGAACTGTTCTTGCTT-3¢Vps4 RKI F 5¢-GCGCTAATGCAACCGATAGATGTCTCTACGGAGGAC-3¢Vps4 RKI R 5¢-GTCCTCCGTAGAGACATCTATCGGTTGCATTAGCGC-3¢Vps4 ... SalIF 5¢-GCCCATATTCGTCGACGCGCTAACAGGTACCAGAGGAGAAGGAGAGAGCGAAGCAAGTAG-3¢Vps4 Dstr R 5¢-GGGCGGATCCTCTGCTTTTCTTTATC-3¢Vps4 Ter1 F 5¢-GCGCTAATGCAACCGTAGTCAATTGATTAACGTGCT-3¢Vps4 Ter1 R 5¢-AGCACGTTAATCAATTGACTACGGTTGCATTAGCGC-3¢Vps4...
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Báo cáo khoa học: "Fully Unsupervised Discovery of Concept-Specific Relationships by Web Mining" pdf
... it participates, without specifying theirtypes in advance. We will generate a concept classand a variety of natural binary relations involvingthat class.An advantage of our method is that ... domains we considered indicatethat, taken as an aggregate, the relations that are gen-erated for a given domain paint a rather clear picture of the range of information pertinent to that domain.Moreover, ... ConstellationsOur seed consisted of two constellation names,Orion and Cassiopeia. There are 88 standardconstellations (www.astro.wisc.edu) some of whichhave multiple names so that the total...
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Báo cáo khoa học: "Fully Abstractive Approach to Guided Summarization" docx
... other reactionsFigure 1: Aspects for TAC’s guided summarization task, category 2: Attacks3 Fully Abstractive ApproachGuided summarization categories and aspects definean information need, and ... a methodology that relies on Informa-tion Extraction and Natural Language Generation,and discuss our early results.2 Guided SummarizationThe stated goal of the guided summarization taskat ... Brief fully abstractive summaries on clusters D100 1A- A, D1039G -A and D1043H -A, respectively on theColumbine massacre, the murder of Theo van Gogh and the assassination of Rafik Hariri.combination...
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Báo cáo khoa học: "Fully Unsupervised Core-Adjunct Argument Classification" pot
... Class-Based Construction of a Verb Lexicon.AAAI ’00.Anna Korhonen, 2002. Subcategorization Acquisition.Ph.D. thesis, University of Cambridge.Hang Li and Naoki Abe, 1998. Generalizing CaseFrames ... allowableframes for each type. Consequently, the commonevaluation focuses on the quality of the allowableframes acquired for each verb type, and not on theclassification of specific arguments in a given ... predicate-slot collocation and argument-slotcollocation.We evaluate against PropBank (Palmer et al.,2005), obtaining roughly 70% accuracy whenevaluated on the prepositional arguments andmore...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ