Báo cáo khoa học: Crystal structure of the parasite inhibitor chagasin in complex with papain allows identiﬁcation of structural requirements for broad reactivity and speciﬁcity determinants for target proteases pptx

... cystatin B N-terminus). (B) Zoom -in view of the interactions of papain with loop L6 of chagasin and loop L2 of cysta-tin B. (C) Zoom -in view of the interactions of papain with loop L4 of chagasin ... afﬁnities for the enzyme. Comparisons of the chagasin papain complex with high-resolutionstructures of chagasin in complexes with cathepsin L, cathepsin B and falci-pain allowed the creation of a ... chagasin and the N-terminal segment of cystatin B. (D) Zoom -in view of the interactions of papain with loop L2 of chagasin and loop L1 of cystatin B.I. Redzynia et al. Chagasin papain complex structure FEBS...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4NLS peptide. The NLS peptide binds to the major binding site in anextended ... cargo binding sites, referred to as the major and minor binding sites [1]. These sites arelocated in the concave face of the protein near regions of invariant Trp and Asn arrays. The major bindingsite ... signiﬁcant interactions with importin-a.This primarily occurs by the formation of a hydrogenbond between the side-chain hydroxyl group of Tyr205 and the importin-a main chain at the C-terminus of the...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... residuesinvolved in the binding of the metal cofactor.ApeSODs in different forms were superimposed with the least square ﬁt of Ca atoms of the residues in the range10–200. ApeSOD and P. shermanii ... metal-binding siteat the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the C-terminal domain ... FEBS of the N-terminal extended region and the followingtwo a-helices, and the globular, (a + b)-type C-termi-nal domain.Oligomeric structure The A ⁄ B and C ⁄ D chains formed dimers in the crystal packing....

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... RHGXRXP and HD, and hydrolyze metal-free phytate with pH optima in the acidic range. They consist of two domains, a large a ⁄ bdomain and a small a domain with the catalytic site at the interface of ... protein. The atomic displacement factors of the PhyK structure aresmaller for the a ⁄ b domain than for the a domain.This has also been observed for other HAPs. The heli-ces directly involved in ... well. The hydrogen bond between the backbone nitrogen atom of Asp291 and the 3-phosphate of the substrate is the only interaction with the protein backbone; all other contacts are formedusing the...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... unlikely, there-fore, that the SEA domain would directly in uence the binding of either substrates or inhibitors into the activesite cleft of the DESC1. Instead, because SEA domainsare proposed ... crystal structure data on the remaining subgroups of the HAT ⁄ HAT-like ⁄ DESC and corin subfamilies exists. We therefore cloned,expressed and puriﬁed the serine proteinase domain of DESC1 and ... confer speciﬁcity to the enzymes for the interactions with the differing substrates and bindingpartners. These loop regions surround the active site and are named according to the residue in the...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... role in the enzymaticreaction which will be discussed later. The structure of the active site, the type of proteinligands, the zinc-binding motif, the presence of a waterFig. 3. Structure of the ... that the metalmust be intrinsically contained in the protein. The structure of the active site is illustrated in Fig. 3. The zinc ion is tetrahedrally coordinated bythree protein residues and ... determination of the BcZBPprotein at a resolution of 1.8 A˚, provides importantclues towards understanding the enzyme function,including the identiﬁcation of a zinc-binding site withina potential...

Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc

... orientation of the aminopyrimidine ring, areconserved in all ThDP-dependent enzymes. One of these, the hydrogen bond between the N1¢ atom of the pyrimidinering of ThDP and the side chain of an invariant ... binds four molecules of the cofactors ThDP and Mg2+. The ThDP binding sites arelocated in narrow clefts at the interfaces formed by the PYRdomains from one subunit and the PP domains of the ... noteworthy that the relativeorientation of the dimers in the tetramer is different in all of the tetrameric PDCs of known three-dimensional structure. Binding of the cofactors ThDP and Mg2+ The homo-tetrameric...

Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf

... determined the F struc-ture containing the truncated region of the C-terminaldomain; however, we failed to determine the structure of the intact glutaminase because the crystals exhib-ited heavy twinning. ... conformations of the regions at100-105 and 303-309 aa in the N form, and we investi-gated the following points. First, these areas are notinvolved in any contacts with other molecules in crys-tals ... Displacement of the backbone atoms of Mglu and Bacillus glutaminase induced by ligand binding. Displacement val-ues for backbone atoms of the nonliganded and liganded structuresare shown. The average and...

Báo cáo khoa học: Crystal structure of the tetrameric inositol 1-phosphate phosphatase (TM1415) from the hyperthermophile, Thermotoga maritima docx

... spheres) and the substrate (D-inosi-tol-1-phosphate, gray). The model of the TM1415 crystal form 1 is in purple and the crystal form 2 in green (including the tartratemolecule). The conformational ... containing the act-ive site. The lower domain is reminiscent of the AMP-binding domain of eukaryotic FBPases (supplementaryFig. S2). The active site is located at the bulge of the C-terminal ... across the entire tetra-mer surface and include some weak contacts in the loop region (residues 22–42). The crystal contacts in form 1 leave sufﬁcient room for unhindered packing of the loops in the...

Báo cáo khóa học: Crystal structure of the chi:psi subassembly of the Escherichia coli DNA polymerase clamp-loader complex doc

... responsible for the stable interaction of single-stranded DNA, SSB and the v:w complex. The N-terminal segment of w is a possible linkerbetween v:w and the clamp-loader In the crystals of v:w, the N-terminal ... that the v:w unit islinked to the single-stranded DNA-binding protein via the distal surface of v. The base of the clamp-loader complex hasan open C-shaped structure, and the shape of the v:w ... located on v,distaltothew inter-action region, and we identiﬁed this as the binding site for aC-terminal segment of the single-stranded DNA-bindingprotein. The other region of sequence conservation...

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