Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx
Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx
... functional domains, to deter-mine the pattern of covalent attachment of substrates and intermediates to active sites during catalysis. Using the natural substrates, the acyltransferaseand acylcarrier ... (AT1)appears as two fragments corresponding to alternativesites of proteolysis; one fragment has a molecular mass of 32582 Da and the second has a molecular mass of 32739 Da. As expected, after ... conformationalchanges during catalysis [22,23]. Both animal FAS and modular PKS are functional homodimers, which raisesadditional questions about the interactions between the active sites of an identical...
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Báo cáo khoa học: The influence of heterodimer partner ultraspiracle/retinoid X receptor on the function of ecdysone receptor pot
... that the EcR ⁄ Locusta migratoria RXR (EcR:LmRXR) heterodi-mers are activated at lower concentrations of ligand when compared with the concentrations of ligand required for the activation of ... response genes and regulatetheir transcription. Most of the nuclear hormonereceptors, including EcR, are modular and function asligand-controlled transcription factors, a characteristicthat ... domain sup-ports ligand-dependent dimerization and transactivationfunctions. A ⁄ B and F domains support ligand-inde-pendent transactivation. The DNA-binding domain and the N-terminal region...
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Báo cáo khoa học: "The clinical value of daily routine chest radiographs in a mixed medical–surgical intensive care unit is low"
... drafting the manuscript.MG participated in analysis and interpretation of the data andin drafting the manuscript. PS, JS and MV contributed to the conception and design of the study and manuscript ... revision.JK was involved in the design and statistical analysis of the study. MS conceived and coordinated the study and wasinvolved in the interpretation of the data and manuscript revi-sion. All ... infiltrates on the CXR; repositioning of tubes in case of malposition of orotracheal tubes (ignoring planned extuba-tions); ultrasound of the thorax in case of pleural effusion on the CXR, start...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
... usuallylack a TATA-box and employ multiple transcriptionalstart sites [10,14]. Mutation of either a CDE or a CHR in a promoter leads to the activation of tran-scription in quiescent cells. A ... regulation. The CDE alone was nottested [41]. The CDE mutation that was assayed wouldalso alter a putative CDE site with the standard dis-tance of four nucleotides to the CHR. With the datapresented ... requires all three CCAAT-boxes andassociation of NF-Y with these elements for optimaltranscriptional activation of the mouse cyclin B2 pro-moter. Changing the distance of the CCAAT-sitesFig....
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: intersubunit interactions during virus assembly doc
... oligomeri-zation affinity of CA and the stability balance of the HIV capsid may be a result, in part, of the preserva-tion of intersubunit electrostatic repulsions.Conformational rearrangements of CAand ... equilib-rium constant of the association step for each singlemutant. It was found that removal of the side chaininteractions of any one of almost half the residues at the interface (Ile150, Leu151, Arg154, ... may also apply to the interfaceas a part of the HIV-1 capsid: a good correlationwas found between the effects on CTD dimerizationand on capsid-like particle assembly of mutationsthat decreased,...
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: designing inhibitors of capsid assembly ppt
... the assembly-competent mutants E18 7A and N18 3A: the conformations of the two assembly-incompetentmutants (Y16 9A and L21 1A) in the absence of CAIare the same as the structures of any mutant whencomplexed to the ... CA assembly.Variants of CAI peptideArmed with this structural information on CAI, Zhanget al. [32] used a structure-based rational designapproach to stabilize the a- helical structure of CAIand ... 7 of NTD form the interface requiredto create a stable hexagonal CA lattice of the virioncapsid [37,38], and these helices pack against a- helices8, 9 (the dimerization helix) and 11 of the...
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Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx
... pro-peptides appear to be diver-gent within the TGFb ⁄ BMP family, and appear tomodulate the function of the mature part by non- covalent association after proteolytic cleavage by pro-hormone convertases ... inducep38 and Smad phosphorylation. The data presented here suggest that the pro-domain of BMP-2 can alter the signalling properties of the growth factor by modulating the ability of the mature part ... association was prolongeduntil a constant maximal response representing an equilib-rium was observed. Signals were then plotted against the pro-peptide concentrations.ELISAFor the assays, a previously...
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Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt
... Walker AL, Iwaki H, Hasegawa Y & Liu A (2005) Kinetic and spectroscopic characterization of ACMSD from Pseudomonas fluorescens reveals a penta-coordinate mononuclear metallocofactor. J Am ... nonoxidative decarboxylation catalysed by a- amino-b-carboxymuconate-e-semialdehyde decarbox-ylase. J Am Chem Soc 129, 9278–9279.20 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada ... correlation between the observed expression profileand the associated variation in QA and PA levels [7],and other investigations have clearly demonstratedthat changes in ACMSD activity are readily...
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... 5¢-GGTATTGAGGGTCGCCATGGTTATGTTCAATCACCA-3¢; reverse primer, 5¢-AGAGGAGAGTTAGAGCCTTACAAGAAGGGTCCAAAGA-3¢). The PCRproduct was purified, treated with T4 exonuclease tocreate vector-compatible overhangs and annealed ... degradation of (GlcNAc)3(r) and (GlcNAc)4( )byLlChi1 8A, illustrated by the production of (GlcNAc)2 during the initial linear phase of the degradation reaction. Note that the enzyme concentrations ... degradation of a- chitin by LlChi1 8A startedwith a rapid phase, regardless of the presence of LlCBP3 3A. In the presence of LlCBP3 3A, the fastinitial phase was maintained longer than in the absenceof...
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Tài liệu Báo cáo khoa học: The intracellular region of the Notch ligand Jagged-1 gains partial structure upon binding to synthetic membranes docx
... mem-branes, was analyzed by CD. As already seen withTFE, at increasing concentrations of SDS J1_tmicundergoes a significant conformational change towardsan a- helical structure, reaching a maximum of ... dependent on pH, sug-gesting that the conformational change is driven by polar, rather than hydrophobic interactions. The partial folding of the cytoplasmic domain of Jagged-1 accompanied by its association ... observe anincrease in the anisotropy and a shift in the maximumfrom 356 to 346 nm upon addition of SDS (final con-centration: 3 mm) but the shift was accompanied by a decrease, rather than an increase,...
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