Báo cáo khoa học: Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin pptx

... 2009 FEBS 3799 Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin Soultana Markopoulou1,*, Evangelos Kontargiris1,*, Christina Batsi1,*, ... vanadium-induced apoptosis of HaCaT cells was mediated by c-fos and involved induction of total Bax and upregulation and accumulation of nCLU. Furthermore, forced expres-sion of nCLU sensitized HaCaT cells ... expression of CLU or b-actin.(E) Cytoplasmic (C) and nuclear (N) extracts isolated from conﬂuentmonolayers of HaCaT Neo and HaCaT c-fos cells and total proteinsisolated from HaCaT cells and HaCaT cells...

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

... theExperimental procedures. This model is strongly sup-ported by previous research on the mechanism of RNA recognition for RRM proteins [6,39,40] and by the structure of existing KH domains bound ... set of protein–RNA decoys for the RNA, DNA and reduced atom distance potentials in decoy discrim-ination.Table S5. Comparison of the performance of thepotential with different upper cut-off ... contactbetween protein and RNA (within a given distancecut-off) was assigned the same score of )1.Docking decoy discriminationAn important property of any potential function is itsability to discriminate...

Tài liệu Báo cáo khoa học: 1,5-Diamino-2-pentyne is both a substrate and inactivator of plant copper amine oxidases ppt

... DABY and PSAO (r ¼ 17) [7]. From thispoint of view, plant CAOs are more resistant to theinactivation by DAPY than by DABY. Binding of DAPYat the active site of GPAO is dependent on both pH and ionic ... recorded by courtesy of Dr Martin Modriansky´ from the Institute of MedicalChemistry and Biochemistry, Faculty of Medicin e, Palacky´ University.Dr Ivo Fre´bort, Palacky´ University, is thanked ... Sayre51Department of Biochemistry,2Laboratory of Growth Regulators, and 3Department of Analytical Chemistry, Faculty of Science,Palacky´University, Olomouc, Czech Republic;4Department of Analytical...

Tài liệu Báo cáo khoa học: What MAN1 does to the Smads TGFb/BMP signaling and the nuclear envelope Luiza Bengtsson pdf

... cell development and differentiation, nuclear anchoring and migration, and apoptosis (reviewed in[1,2]). Mutations in several of the integral membraneproteins of the inner nuclear membrane ... hyperostosis is accompanied by abnormalities of adjacent soft tissues, such as jointcontractures, sclerodermatous skin lesions, muscleatrophy, hemangiomas and lymphoedema [17]. Thedisease causing ... pathways results in developmental disor-ders, cancer, ﬁbrosis and autoimmune disorders. Signa-ling is initiated by binding of the cytokine to ahomodimeric complex of cytokine receptor type II,which...

Tài liệu Báo cáo khoa học: Evidence for proteasome dysfunction in cytotoxicity mediated by anti-Ras intracellular antibodies pdf

... undergo apoptosis (Fig. 1 and Table 1). Thisprocess is accompanied by aggresome formation in the vastmajority of transfected cells and marked inhibition of proteasome function. In the case of anti-(Ras ... apoptosis in a high percentage of trans-fected cells and inhibit cell growth in different cell lines. Thisphenomenon is accompanied by the formation of aggre-somes and recruitment of proteasomes ... suspension, and 20 · 104 cells were seeded into three 100-mm tissue culture dishes.After 15–20 days of G418 selection, cells were ﬁxed and stained with a solution of 0.4% Coomassie blue and 50%2-propanol...

Báo cáo khoa học: a-Fetoprotein positively regulates cytochrome c-mediated caspase activation and apoptosome complex formation docx

... Bcl-2 and Bcl-xL, resulting in tumor-speciﬁc sup-pression of apoptosis and enhancement of the malignance and therapy resistance of tumors [39–43]. The existence of ahigh background level of ... release of mitochondrial cyt-c in HepG2 cells Our previous publications were devoted to the study of AFP-induced apoptosis in whole cells and suggested thatthis mechanism is independent of membrane ... cascade. To understand the molecular mecha-nisms of AFP -mediated apoptosis signaling, we establisheda cell-free system, similar to that used for studies of cyt-c-induced apoptosis [28,29]. We...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... control of gene expression. Aseach step of the RNA metabolism is tightly regulated,the regulation of mRNA export, stability and transla-tion rate is essential for the control of the expression of ... the nuclear fraction of ASF ⁄ SF2 is phosphorylated [31,32] and ASF ⁄ SF2 nuclear export is conditioned by a dephosphorylationprocess [ 33,34], we analyzed th e sub-cellular distributio n of ... molecularprocesses. However, the physiological existence of ASF ⁄ SF2-TIA complexes is conditioned by the tissuedistribution of TIA protein [46], which is clearly morerestricted compared to ASF ⁄...

Tài liệu Báo cáo khóa học: The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life pptx

... Ban and his collaborators (ETH, Zurich) for supplying the plasmids withtRNASecsynthetic genes. This work was supported by grants from theMinistry of Science and Technology of the Republic of ... kinetics of serylation and tRNA discrimination by the two SerRSs that coexist in methanogenic archaeonMethanosarcina barkeri [16] (D. Korencic, unpublisheddata). One of these enzymes is bacteria-like ... 20A, which is present in the majority of tRNASerisoacceptors from bacteria and eukaryotes,including serine-speciﬁc tRNAs from the organelles, is oftenmissing in the sequences of methanogenic...

Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf

... Tfu,Tfus02000308 of Thermobiﬁda fusca.TheRv2228c of M. tuberculosis is identical toMb2253c of Mycobacterium bovis.N. Ohtani et al. A fusion protein consisting of RNase H and APaseFEBS Journal 272 (2005) ... examined here is a bifunctional enzyme con-sisting of an RNase H domain and an APase domain, and it is a novel style in the Type 1 RNase H family.Experimental procedures Cells, plasmids, and materialsThe ... cata-lysis [29,33]. They instead utilize histidine to form anenzyme–phosphohistidine intermediate, which is essen-tial for their catalysis [33,34]. A His residue in anRHGXRXP motif that is highly...

Tài liệu Báo cáo khoa học: "A Mobile Touchable Application for Online Topic Graph Extraction and Exploration of Web Content" ppt

... chunk–pair–distance model is computedfrom the list of chunks. This is done by traversingthe chunks from left to right. For each chunk ci, aset is computed by considering all remaining chunks and ... distance to ci, i.e., (ci, ci+1, disti(i+1)),(ci, ci+2, disti(i+2)), etc. We do this for each chunklist computed for each web snippet. The distancedistij of two chunks ci and ... For-mally, a topic graph T G = (V, E, A) consists of aset V of nodes, a set E of edges, and a set A of nodeactions. Each node v ∈ V represents a chunk and is labeled with the corresponding PoS–tagged...

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