... compilation ª 2009 FEBS
Essential role of the C-terminus in Melanocarpus
albomyces laccase for enzyme production, catalytic
properties and structure
Martina Andberg
1
, Nina Hakulinen
2
, Sanna Auer
1
, ... show the critical role of the last
amino acids in the C-terminus of MaL. Deletion of the
four last amino acids strongly reduced the ex...
... in obese mice
In order to gain insights into the potential biologically
relevant role of miR-27 in the regulation of adipose tis-
sue functions in vivo, we examined the expression of
miR-27 in ... together, these results illustrate
a critical and specific role of miR-27 in the regulation
of adipogenic differentiation.
miR-27 prevents the induction of PPA...
... molecule
comprising Ôresidues in the protruding domain consisting
of the type VI b-turn and the first part of b-strand 3Õ (i.e. the
b-hairpin loop and part of the adjoining b-sheet) and
Ôresidues in the loop ... obtained by other groups
who also point to the role of the connecting loop of the
b-hairpin of defensins. A combination of mutational
analys...
... gene. Therefore, both free pregnenolone
production and the efficient coupling of the SCC and the
3b-HSD activities in yeast require the use of strains lacking
this acetylating activity. For this ... targeting to the PM.
For example, the mature forms of the human, bovine and
pig CYP11A1 have two positively charged residues at their
NH
2
termini that could favor t...
... acetyl-transferase-catalyzing
inner domain. E3BP domains: L3, N-terminal lipoyl domain; B, E3
binding domain; I, inner domain which associates with the inner
domain of E2. Dotted connections indicate binding interactions ... running in opposite direc-
tions. The monomer association involves the ATP-using
C-terminal domains interacting near the base of that side of
the wedge...
... determined using
the bicinchoninic acid protein assay kit (Pierce, Rockford,
IL, USA) with BSA as the standard.
Kinetic parameters for the enzymatic reaction
of P-gp
For rhodamine 123, colchicine, ... that the linker region maintains the
tightness of coupling between the ATP hydrolase reaction and substrate
recognition. Inspection of structures of the P-gp homolog,...
... orientations in the
membrane; the N terminus of the N-terminal domain
is extracellular, while the N terminus of the C-terminal
domain is intracellular. In the [st326]2HCT family,
TMS I is not part of the ... families and the N- and C-ter-
minal halves of ST3 (open bars), between the N-terminal halves of
ST3 and the C-terminal halves of ST3 (grey bars)...
... quantified using the Scion Image program. The
upper numbers represent the intensity of each band normalized to the
band in lane 1. The lower numbers represent the intensity of the bands
in lanes 5 and ... for initiation both in vitro (Fig. 3A) and in vivo
(Fig. 3B) when the R2 5¢-UTR is either deleted or mutated.
In contrast, the in vivo results suggest...
... that in all cases,
the PC is linked through the 6-hydroxyl of GlcNAc.
Some PC-containing structures were also putatively
modified by fucose; thus, the linkage and the position
of the fucose in these ... resulting in a loss of productivity.
Whereas the large adult roundworms reside in the gut,
the larvae hatching from ingested eggs travel from the
stomach or sm...
... point
of view, they allow the cell to dispose of excess
metabolites by storing them in an inert form. When
demand for these metabolites is high, they are read-
ily available without the need for ... quantitatively the role of TAG
degradation in growing cells and the metabolic flux of
FA. We reconstructed the metabolic pathway of TAG
lipolysis in yeast in sil...