Báo cáo khoa học: Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica pdf

... FEBS 3395 Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica Erika Pineda1, ... 2010)doi:10.1111/j.1742-4658.2010.07743.xThe in vitro Entamoeba histolytica pyruvate:ferredoxin oxidoreductase (EhPFOR) kinetic properties and the effect of oxidative stress on glycolyticpathway enzymes and ﬂuxes in live trophozoites ... against oxidative stress damage, have not been reported. In vivo effects on glucose-fermenting enzymes and ﬂuxes under oxidative stress Recently, we reported that amebas incubated for 30 min in...

Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx

... direct interac-tion between HSP70 and Smac proteins, and theATP-binding domain of HSP70, rather than the pep-tide-binding domain, was essential for this speciﬁcfunction of the protein. Recent ... that the ATP-bindingdomain of HSP70 is essential for its anti-apoptotic role. For example, deletional analysis demonstrated thatthe ATP-binding domain is essential for inhibitingthe release ... theATP-binding domain of HSP70 is critical for sequester-ing AIF in the cytosol [29]. In the present study, wedemonstrated that the ATP-binding domain of HSP70was indispensable for inhibition...

Báo cáo khoa học: Perturbation of folding and reassociation of lactate dehydrogenase by proline and trimethylamine oxide potx

... were incubated on ice for 60 min. Allincubations, for both inactivation and reactivation, wereperformed in polypropylene tubes.ReactivationRenaturation following unfolding in GdnHCl was initiatedby ... Each of the lines shown for prolinerepresents a single experiment, the points for the line for controls reflectfive independent determinations.Ó FEBS 2003 Perturbation of protein folding by osmolytes ... during the first 5 h afterinitiation of reactivation. Each of the lines shown for proline representsa single experiment, the points for the line for controls reflect fiveindependent determinations....

Báo cáo khoa học: A hydrophobic segment within the C-terminal domain is essential for both client-binding and dimer formation of the HSP90-family molecular chaperone pptx

... identiﬁcation ofthe minimal region of the C-terminal domain for the clientbinding. Bearing in mind the fact that the 35-amino-acidresidues corresponding to the C-terminus of HSP90 are deleted in HtpG, ... domain and the C-terminal domain.Similarly, the C-terminal 326 amino acids of barley GRP94[22] and 200 amino acids of canine GRP94 [27] are sufﬁcient for the dimer formation. However, Wearsch and ... proposedto be involved in the interactions with such target proteins. For instance, a highly charged region of chick HSP90 (aminoacids 221–290) is essential for the binding to estrogen and mineralocorticoid...

Báo cáo khoa học: "Combining Lexical, Syntactic, and Semantic Features with Maximum Entropy Models for Extracting Relations" pptx

... LDCinto separate training and development sets. Thetraining set contained around 300K words, and 9752instances of relations and the development set con-tained around 46K words, and 1679 instances ... 2004) for more details of our men-tion detection and mention chaining modules. In thenext section, we describe our extraction system. Wepresent results in section 3, and we conclude aftermaking ... extract-ing relations that combine diverse lexical, syntactic and semantic features. Our results indicate that us-ing a variety of information sources can result in improved recall and overall F measure....

Báo cáo khoa học: Reduction of S-nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry pptx

... double focussing sectorsof the instrument (MS1) focussing them into an inter-mediate collision cell containing Xe and orthogonallyaccelerating undissociated precursors and fragment ionsinto the ... [31]. For HMGSH, anexact positioning in the active site is essential for efﬁcientcatalysis. The substrate binding is accomplished in part by acharge interaction between Arg115 and the glycine ... estimated to be in the range of 50–100 lM in thehepatocyte cytosol and even lower in other tissues, includingheart and brain [39], indicating that NADPH is unlikely tobe utilized in vivo by ADH3...

Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx

... reoxygenation but is ineffective atmaintaining glutathione levels during hypoxiaGlutathione is one of the main antioxidant compounds in the cell, and it is also essential for DNA synthesis and repair ... protective effects against hypoxia and reoxygenation. This is mainly ascribed to its ability tomaintain redox status [17], intervening in the DNArepair system [18–20] and restoring antioxidant capaci-ties ... CytosolGlutamate5-Oxoprolineγ-Glutamyl-cysteineCysteine GlycineADP + Pi + ATP + ATPADP + Pi GlutamineGS-X(conjugate)Amino acids (AA)γ-Glutamyl-AACysteinyl-glycineγ-Glutamyl-AACysteinylglycineconjugateExtracellular...

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... mitogen-activated protein kinase kinase, in vitro and in vivo: analysis of active mutants expressed in E.coli and generation of the active form in stress response in seedlings. Plant J 29, 637–647.40 ... of the mitogen-activated protein kinases (MAP-Ks), MPK3 and MPK6, in response to oxidative stress. In addition, theserine ⁄ threonine protein kinase Pto-interacting 1-2 (PTI1-2) has beenpositioned ... protein kinase; MAPKK, mitogen-activated protein kinase kinase; MBP, myelin basic protein; OXI1, oxidative signal-inducible 1; PDK1, 3-phosphoinositide-dependent kinase 1; PTI1, Pto-interacting...

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... promoting an increase in proopiomelanocortin and cocaine and amphetamineregulated transcript. Taken together, these ﬁndingsprovide a compelling argument for the role for malo-nyl-CoA in regulating ... as other sites, are known to reach the CNS to effect changes in feedingbehavior and energy expenditure. Thus, insulin, leptin and ghrelin, as well as other hormones ⁄ cytokines,interact with ... maintaining a lower foodintake. This is accompanied by a suppression in energy expenditure. These data suggest that CPT1c canintegrate carbohydrate and lipid nutrient sensing in thebrain and...

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... ATP-binding pocket. Pantoatebinding to the ATP-binding site induced large changes in structure, mainly for backbone and side chain atoms of residues in the ATP bindingHXGH(34–37) motif. Sequence-speciﬁc ... panto-ate-binding and ATP-binding sites of PS [11]. ATPcaused additional changes in the spectrum, particularly for those residues that lie in the ATP-binding pocket.Interestingly, in the presence ... Srini-vas for help with preparing the clone of nPS, A. Aro-ra, CDRI, Lucknow, for help in collecting therelaxation data, and N. Srinivasan and S. Yamunadevi for their help with modeling and...

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