Báo cáo khoa học: Delineation of the roles of FadD22, FadD26 and FadD29 in the biosynthesis of phthiocerol dimycocerosates and related compounds in Mycobacterium tuberculosis pptx
Báo cáo khoa học: Delineation of the roles of FadD22, FadD26 and FadD29 in the biosynthesis of phthiocerol dimycocerosates and related compounds in Mycobacterium tuberculosis pptx
... representation of the roles of the FadD proteins encoded by the DIM + PGL locus in the biosynthesis of DIMs and related compounds in Mycobacterium tuberculosis. The role of the activation enzymes FadD22, FadD26 ... knowledge of the bio-synthesis of DIMs and PGLs, the in vivo functions of these FadD proteins in this pathway remain to beestablished and ⁄ or completed. For instance, the involvement of FadD26 in the ... of these proteins in the activation of the various intermediates in DIM and PGL biosynthesis has not been analyzed. This study was undertaken todissect the roles of FadD22, FadD26 and FadD29 in the...
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Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt
... shown). The basic cluster of amino acids in the bindinggroove of 14-3-3, including amino acids Lys-49, Arg-56, Lys-120 and Arg-127, in an otherwise acidic mole-cule, are important for the interaction ... forphosphorylation-independent binding The interaction between 14-3-3 and ExoS is importantfor the ADP ribosylation activity of ExoS and evenmore intriguingly, appears to be independent of serine-phosphorylation ... activity in vivo. We have approached these questions using astrategy of single amino acid site-directed mutagenesis of the cofactor interaction domain within ExoS.Various single mutant ExoS proteins...
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Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx
... of the input and the eluate of the chromatographywere resolved by Tricine ⁄ SDS ⁄ PAGE and then analyzed by Coo-massie Brilliant Blue staining (left) and western blotting (right).Lane 1: input ... to those of bombyxin and insulin,which are of the order of 100 pm [23,24]. The veryhigh titer of 8K-BLP may be due to a remarkably highlevel of gene expression in the fat body and to the large ... suggesting that this peptide may alsoaffect the development of the wing disks at the prepu-pal stages. It is interesting to note that bombyxin-IIalso stimulated the growth of the wing disks in the butterfly...
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Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx
... that the most characteristic signatures in the sequences are present in both proteins, tyrosinases and catechol oxidases; these include the six histidine resi-dues involved in the binding of a ... browning of fruits and vegetables, and morphogenesis and fruiting body formation in fungi.All of these processes involve melanin formation. In the bacterial kingdom there are some examples of ... signatures of tyrosinases, including the CuA and CuB binding sites to ligand the copperTable 3. Kinetic parameters for the two PPOs. The enzymes wereobtained from extracts of R. solanacearum strains...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... mechanism of the deamination reaction and the subsequent metabolism, including the deaminationstep. The product formed from 4-amino-3-hydroxybenzoicacid by the action of purified 4-amino-3-hydroxybenzoate2,3-dioxygenase ... of the purified enzyme at 266 nm. The typicalprotein absorption p eak of 2 80 nm shifts to 265 nm if the protein contains a flavin-type cofac tor [28]. We failed toidentify the cofactor of the ... enzyme The activity of the enzyme present in the crude extract of strain 10d that used 2 -amino-5-carboxymuconic 6-semi-aldehyde as substrate was measured by monitoring the increase in the absorbance...
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Tài liệu Báo cáo khoa học: "STRING-TREE CORRESPONDENCE GRAMMAR: A DECLARATIVE GRAMMAR FORMALISM FOR DEFINING THE CORRESPONDENCE BETWEEN STRINGS OF TERMS AND TREE STRUCTURES" pdf
... representation is defined as a mapping between elements of the set of substrings of the string and elements of the set of subtrees of the tree : such a relation is called a string- tree correspondence. ... substrings of the related sentence The characteristic of the linguistic approach is the design of the multilevel structures, while the grammar formalism is the tool (notation) for expressing these ... for the representation of the interpreta- tion of the sentences, and the (possibly non-pro- jective) correspondence between subtrees of each tree and substrings of the related sentence. The...
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Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s fluorescent probe for the determination of glutathione doc
... proceed for 10 min, and the fluorescence was then measured at room tem-perature. In uence of interference The in uence of the main constituents of plasma on the determination of 2.00 · 10)6molÆL)1GSH ... metallobridgedbis(b-CD)s 2 The in uence of the concentration of 2 on fluorescenceintensity is shown in Fig. 4. As can be seen, as the concentration of 2 increased, the fluorescence intensity of the system also increased ... solution and showed high bindingability because of the two adjacent bis(b-CD)s.Because of the conformation of the linker of 1, the nitrogen atoms and amido bond formed two chelaterings to coordinate...
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Báo cáo khoa học: Sensitivity to Hsp90-targeting drugs can arise with mutation to the Hsp90 chaperone, cochaperones and plasma membrane ATP binding cassette transporters of yeast docx
... contacts the C-terminus of Hsp90 [48], its binding alsodisplaces bound GA, indicating that there is also aninteraction of Sti1p with the ADP/ATP binding site of the chaperone [33]. The increased ... ADP/ATPbinding site, thereby inhibiting the ATP binding step of the Hsp90 chaperone cycle [6–9].Interest in Hsp90-targeting drugs as possible anticanceragents was triggered initially with the identification ... through their ability to bind within the ADP/ATPbinding pocket of the chaperone. Drugs based on these compounds are now being developed as anticancer agents,their administration having the potential...
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Báo cáo khoa học: "Combining a Statistical Language Model with Logistic Regression to Predict the Lexical and Syntactic Difficulty of Texts for FFL" potx
... for the logistic regression model. More informationabout the origin and smoothing of the probabilitiesis given in Section 6.4.2 Measuring the tense difficultyHaving considered the complexity of ... 1),α1 and β1= 0. Thus, when looking for the proba-bility of a text belonging to the baseline level, it iseasy to compute the numerator, since exp(0) = 1. The value of the denominator is the ... p(ti) is the probability of meeting the token ti in French; and n is the number of tokens in a text.2. Deciding what is the best linguistic unit toconsider. The equations introduced above use21tokens,...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing docx
... transactivation domain. The DNA-bind-ing domain is responsible for DNA binding specificity and dimerization, and the ligand-binding domain isresponsible for binding of the ligand and associatedinduced ... splicing of the E2-responsivegene, oxytocin [90]. NRs also interact with the SR(serine- and arginine-rich proteins) family of splicingfactors. CAPER, a SR-like protein, interacts with ERa and the ... transformation.Thus, diverse DNA-binding domains, protein–proteininteraction modes and pre-existing chromatin modifica-tions may facilitate the binding of MLLs, depending on the context and cell types, to...
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