... coronaria
Raka Ghosh, Sibani Chakraborty, Chandana Chakrabarti, Jiban Kanti Dattagupta and
Sampa Biswas
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, ... S, Sundd M, Jagan-
nadham MV & Dattagupta JK (1999) Crystallization
and preliminary X-ray analysis of ervatamin B and C,
two thiol proteases from Ervatamia coronaria. Acta
Crystallogr D 55, ....
... the yeast cytochrome
bc
1
complex based on analysis of single and double deletion mutants
lacking supernumerary subunits and cytochrome
b
Vincenzo Zara
1
, Ilaria Palmisano
1
, Laura Conte
1
and ... Electro-
phoretic analysis of DNA on agarose gels, restriction
endonuclease analysis, ligation of DNA fragments,
Table 1. Yeast strains used in this study.
Strain Genotype Reference
W303–1 A (...
... experiments revealed that ADP signifi-
cantly activates OfODC, whereas ATP was only a
weak activator [3]. Although the mechanism of activa-
tion by ADP remains to be elucidated, the authors
postulated its ... physiological relevance. To date, more
than 50 oxalotrophic bacteria that are capable of using
oxalate as a carbon and energy source have been iden-
tified [6]. The Swiss-Prot ⁄ TREMBL...
... City, CA, USA).
Coagulation assay
APTT assays were carried out on an automatic coagulation
analyser (Hemostasis Analyzer KC-1; Sigma Diagnostics,
St Louis, MO, USA) according to the manufacturer’s ... G & Rosing J (2001) Snake venom activators of
factor X: an overview. Haemostasis 31, 225–233.
8 Takeya H, Nishida S, Miyata T, Kawada S, Saisaka Y,
Morita T & Iwanaga S (1992) Coagulatio...
... compounds,
the parameter k
a
was calculated. As shown in the Materials
Table 2. Parameters characteristic of the interaction of rhodamines with
cells. The data are the means ± SEM of three determinations. ... substrate
transport, is a powerful approach to enhancing our
understanding of their function and mechanism. In this
paper, we present data that further characterize the
transport of...
... sterile water.
The cDNA was ligated into the Uni-ZAP XR vector
(Stratagene) using T4 DNA ligase at 4 °C for 2 days and
then packaged into Gigapack III Gold Packaging Extract
(Stratagene) by incubating ... Chemical Co.) for 1–5 min.
Bacteriostatic assays
Bacteriostatic assays were carried out essentially as in
Biswas et al. [28]. LB media (1 mL) was inoculated with
DH 5a E. coli and incub...
... k
3;ASS
k
5;ASS
k
7;ASS
k
9;ASS
k
11;ASS
ATP(t) Asp(t) þ k
2;ASS
k
5;ASS
k
7;ASS
k
10;ASS
k
12;ASS
Asp(t) AMP(t) (as(t)
þ asR(t)) + k
1;ASS
k
3;ASS
k
5;ASS
(k
7;ASS
k
9;ASS
þ k
7;ASS
k
11;ASS
+ k
9;ASS
k
11;ASS
) ... The construction of detailed, and
necessarily complex, mathematical models serves as a ÔtoolÕ
to facilitate analysis of channelling in biochemical pathways
like the urea cyc...
... derive an overall measure of likelihood
of a partial analysis. Measures are included for the
likelihood of that particular reading of the word and
for aspects attached to an impulse: a) for ... ~xical tasks, extension tasks,
insertion tasks and virtual tasks. A lexical task specifies
53
a possible reading era word to be introduced in the chart
as an inactive edge. An extension ta...
... Canyuk B, Focia PJ & Eakin AE (2001) The role for
an invariant aspartic acid in hypoxanthine phospho-
ribosyltransferases is examined using saturation muta-
genesis, functional analysis, and ... 6615–6627.
24 Raman J, Sumathy K, Anand RP & Balaram H (2004)
A non-active site mutation in human hypoxanthine
guanine phosphoribosyltransferase expands substrate
specificity. Arch Biochem Bi...
... regulation and mixed lineage
leukemia.
Acknowledgements
We thank Imran Hussain, Sahba Kasiri, Bishakha
Shrestha, Saoni Mandal and other Mandal lab mem-
bers for useful discussion and critical comments. ... gene
activation
A widely studied NR coactivator is activating signal
cointegrator-2 (ASC2, also named AIB3, TRBP,
TRAP250, NRC, NCOA6 and PRIP) [69]. ASC2 is a
coactivator of multiple n...