... observation of the protein’sinfluence on the progression of the cell cycle and the silica biogenesis. As the regulation of NR expressionappears to be very similar throughout the diatomrealm [12,13], the ... genes.Furthermore, the addition of both nitrate and appro-priate concentrations of ammonium allows, within lim-its, the control of the amount of induced protein. The observed lag phase of 1 h for Pnr ... demonstrated that the 5¢-UTR and 3¢-UTR of CfNR allow control of both the timing and level of expression of introducedgenes in transgenic C. fusiformis.ResultsIncreasing the efficiency of C. fusiformistransformationPreviously,...