Báo cáo khoa học: Thermosynechoccus elongatus DpsA binds Zn(

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): a unique metalloprotein ppt

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modiﬁcations and in enhanced protective activity in sepsis doc

... binding as detected at A 405 nm . The results represent the average of at least three independent measurements, with the standard deviation indi- cated by error bars (gray bar, native Z2 antibody; ... by the Animal Care Commission of the Institute of Microbiology, in accordance with National and European Regulations. The number of ani- mals used was kept at the...

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx

... intermediate. We also propose that the seven-fold higher efﬁciency of RNA cleavage by RNase Sa than by RNase Sa2 can be at least partly explained by the Val43 (RNase Sa) to Arg45 (RNase Sa2) substitution. ... nucleotide. Discussion The goal of the present work was to better understand the catalytic mechanism of RNase Sa2 and to account for the differences in catalytic activity...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf

... agreement with those against thermal denaturation. Stabilization of 4 · -RNase HI with Arg97 fi Gly and Asp136 fi His mutations To examine whether the Arg97 fi Gly and Asp136 fi His mutations stabilize ... into So-RNase HI or 4·-RNase HI. The stabilities of the 4·-RNase HI, 5·-RNase HI and 6·-RNase HI proteins against thermal denaturation were analysed as described for R97G-...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Hypoxia downregulates farnesoid X receptor via a hypoxia-inducible factor-independent but p38 mitogen-activated protein kinase-dependent pathway doc

... Saitou 1 , Takehisa Atsumi 1 , Takashi Sato 1 , Ken Ando 1 , Shuntaro Hara 2 , Kiyomi Kikugawa 1 and Makio Hayakawa 1 1 School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan 2 ... mutagenesis by PCR as follows. The pBluescript SK vector (Stratagene, La Jolla, CA, USA) encompassing an HIF- 1a cDNA in which Pro564 and Asn808 were replaced with alanines was used...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Hypoxic resistance to articular chondrocyte apoptosis – a possible mechanism of maintaining homeostasis of normal articular cartilage pdf

... the combined effects of ALLN and TRAIL in canine chondrocytes, western blot analysis was performed. It was shown that cotreatment of cells with ALLN and TRAIL increased caspase-8 activation and ... demonstrated that ALLN pretreatment with TRAIL increased the activation of caspase-8 under normoxic conditions, but that caspase-8 activation was inhibited under hypoxic conditions. I...

Ngày tải lên : 18/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Pyrimidine-speciﬁc ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc

... via two engineered restriction sites (NdeI and EcoRI) introduced by PCR with the primers 5¢-GCTATTGTGGTAGAATA CATATG AGACAC-3¢, sense, and 5¢-GGAGTTGTAAAAATTC GAATTCTAAAGAGC-3¢, antisense (the ... culture and homogenate preparation Sulfolobus solfataricus was isolated from an acidic hot spring in Agnano (Naples, Italy) and grown at 87 °Cas previously reported [51]. For the ho...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Redox regulation of dimerization of the receptor proteintyrosine phosphatases RPTPa, LAR, RPTPl and CD45 pdf

... receptor protein-tyrosine phosphatases (RPTPs) have two intra- cellular PTP domains. The membrane proximal domain (D1) contains most catalytic activity, whereas the membrane distal domain (D2) has a regulatory function ... to their ectodomains. To investigate the role of the cytoplasmic domain in dimerization we generated RPTPa ectodomain (EDa) ⁄ RPTP chimeras and found that – s...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

... Colavita AR, FitzGerald GA & Violi F (1997) Platelet activation by superoxide anion and hydroxyl radicals intrinsically generated by platelets that had undergone anoxia and then reoxygenated. ... cyto- plasm to the plasma membrane, where it associates with 5-LOX-activating protein (FLAP) and metaboli- zes arachidonic acid to release ROS [28,29]. The phos- phatidylinositol-3-ki...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt

... determined at about 10-fold lower protein concentration than the measurements of inactivation, show the same trends with respect to the effects of NaCl, CaCl 2 and EDTA as the inactivation constants. As ... plot of the data from (A) in the range 0–100 m M Ca 2+ . (C) Modiﬁed Scatchard plot of the data from (B). DA corresponds to the change in relative activity. The...

Ngày tải lên : 19/02/2014, 02:20

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Báo cáo khoa học: Thermosynechoccus elongatus DpsA binds Zn(II) at a unique three histidine-containing ferroxidase center and utilizes O2 as iron oxidant with very high efﬁciency, unlike the typical Dps proteins ppt