Báo cáo khoa học: Small peptides derived from the Lys active fragment of the mung bean trypsin inhibitor are fully active against trypsin pptx
Báo cáo khoa học: Small peptides derived from the Lys active fragment of the mung bean trypsin inhibitor are fully active against trypsin pptx
... Small peptides derived from the Lys active fragment of the mung bean trypsin inhibitor are fully active against trypsin Rui-Feng Qi1, Zhi-Xue Liu2, ... studiedBowman–Birk protease inhibitors. A modified peptide, Lys3 3GP, with 33residues derived from the long chain of the Lys active fragment of mung bean trypsin inhibitor, was successfully expressed in ... to the active core of the fragment, was synthesized. Both the recombinant and the synthesized peptides had the same inhibitory activitytoward trypsin at a molar ratio of 1 : 1, implying that the...
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Báo cáo khoa học: A peptide derived from cyclin-dependent kinase activator (p35) specifically inhibits Cdk5 activity and phosphorylation of tau protein in transfected cells pdf
... constitute the smallest fragment (p16) of p35 that fully activates Cdk5 [20](Fig 1A). That study found that further truncation of p16,removing either the N-terminal 11 residues (part of the p35 ... appears to be aconsequence of the rigidity of the cyclin box structure of the activator compared to the relatively more flexible T-loop.Judging from the crystal structure, the two criticalsegments ... °C, the protein concentrations of Fig. 1. Identification of the Cdk5/p25 inhibitory peptide (CIP) derived from p35. (A) Mapping of p25, p16 and CIP to p35 (human sequence).Red segments are the...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... A common drawback of such approachesis the cost of the affinity resins used to isolate the fusion protein and the proteases required to liberate the protein of interest from the fusion protein. ... concentrated mannerat the top of the column, or, if bound to the resinprior to packing the column, the salt gradient concen-trates the peptide on its way out of the column. The molecular mass ... to smaller losses than gel filtration due to morerapid handling using the devices and loss of peptide on the column resin. The only detriment of the peptides produced here is the fact that they...
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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... when the CABS charged cluster or the LBD are mutated are not simply due to decreased proteinexpression or stability. Neither mutation of the CABSVH2-C domain charged cluster nor deletion of the LBD ... pro-teins, but we expect that the relative expression level of the tagged proteins is indicative of that of the equival-ent untagged proteins.To assess the function of these proteins in endo-cytosis ... membranes would restore function in the absence of the LBD. C-Vrp1p364)760was tagged at the C-terminuswith the CAAX box of S. cerevisiae Ras1p [45]Fig. 5. The LBD of C-Vrp1p is essential for cortical...
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Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
... antibody raised against KLK11 was the same sizeas that of the translational product by isoform 2mRNA (Fig. 4B). The product by isoform 1 mRNAwas smaller than the isoform 2 product. The multiplebands ... isoforms 1 and 2 are detected in the organ. All of these nucleotide sequenceswere identical to the genomic sequence of the humanKLK11 gene (GenBank accession number AF164623).We compared the ... below). The transcriptional initiation sites of exons 1a and1c are located 1163 bp and 1510 bp, respectively,downstream from the transcription terminator of the KLK12 gene. The promoter region of the...
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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... mediates activation of Ras subsequent to the stimulation of Gi- and Gq-coupled receptors [8,9]. The main mechanism for the activation of Ras-GRF1 is the binding of Ca2+⁄ CaM to the N-terminalIQ ... identified [17,19]. The smaller iso-forms correspond to N-terminal deletions of the full-length 140 kDa protein. The physiological role of the guanine nucleotide exchange activity of the truncatedforms ... truncatedforms is not known as they are missing the Ca2+⁄CaM-binding IQ domain that is involved in the activa-tion of Ras-GRF1.Stimulation of all the splice variants of the Gs-coupledserotonin...
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Tài liệu Báo cáo khoa học: "Exploiting Readymades in Linguistic Creativity: A System Demonstration of the Jigsaw Bard" docx
... the scale of the Bard’s genera-tivity, and the quality of its insights.5 Empirical Evaluation The vastness of the web, captured in the large-scale sample that is the Google ngrams, means the Jigsaw ... properties are indeed acquired from the Web for our vocabulary of stereotypes(this point relates to the aptness of the similes sug-gested by the Bard); and second, that the adjectivesconnected by the ... the first case, predictions of the pleasantness of stereotype nouns based on the pleasantness of theirsalient properties (i.e., predicting the pleasantness of Y from the Xs in “as X as Y”) have...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... coreceptors, are m ainlyregulated at the transcriptional level. This report s hows that the b FGF r egulation of their expression speci®cally dependson the nature of HSPG and of the Sertoli ... (HSPG) on the cell surface [11].Oligosaccharidic sequences of HS chains are de®ned for the bFGF binding and for the recognition of the speci®c bFGFreceptor, leading to the formation of a ternary ... sulfated sequence of theirheparan sulfate chains [34].Sodium chlorate is an inhibitor of ATP sulfurylase andhence of the production of phosphoadenosine phospho-sulfate (PAPS), the active s ulfate...
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Báo cáo khoa học: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis docx
... regulates the formation of transport complexes.GTP hydrolysis by Ran in the cytoplasm drives disso-ciation of the export cargo from the export complex,whereas RanGTP promotes disassembly of the ... regulates the move-ment of molecules into and out of the nucleus and isnecessary for the survival of eukaryotic cells. The pas-sage of molecules such as RNA, proteins and ionsacross the nuclear ... result of adifference in the ratio of the bleached nuclear area to the total cell volume (Doc. S1B).Nuclear export appears to be more active inmyotubes than in myoblastsWe next compared the...
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Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
... linkers The initial analysis of 40 amino acid sequences of GH 13members having the ÔclassicalÕ SBD (Table 1) revealed that,in fact, there are two groups of these enzymes, which are the five-domain ... agreementwith the complete-sequence tree (Fig. 3A). In the group of CGTases produced by the genera Bacillus and Thermo-anaerobacter (the large compact clusters in the CGTasesgroup of the trees), the ... sequence identity for the SBD from B. circulans strain 251 CGTase with the SBDs from the CGTases from Thermococcus sp. B1001, Bacillusohbensis,andThermoanaerobacter thermosulfurogenes are 37.3%, 63.8%...
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