Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc

... formation and identify a region, located within the C-terminal domain of Ure2p that interacts with Ssa1p. Because the C-termi-nal domain of Ure2p is tightly involved in the assembly of the prion into ... His-Tagged Ssa1p (B). (A) A mixture of untreated Ure2p and Ssa1p (lane 1); Ure2p alone(lanes 2, 5, 8 and 11), Ssa1p alone (lanes 3, 6, 9 and 12), and Ure2p incubated in presence of Ssa1p (lanes ... Journal 277 (2010) 5112–5123 ª 2010 The Authors Journal compilation ª 2010 FEBS 5123 A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of...

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... cell intoxication, diphtheria toxin is cleaved by furin into two fragments: the A chaincorresponding to the catalytic domain (C domain) ; and the B chain corresponding to the translocation domain ... (N) state. Thus, the MSapproach used in this work offers a valuable alternative. The data enabled us to identify the core of the pro-tein in the N- and MG states, the regions of moderate and ... structure. Within the wholetoxin, TH5 is partly covered by the C-terminal part of the receptor (R) domain [2,3]. Within the isolated T domain, this helix is likely to have one face at the pro-tein...

Báo cáo khoa học: Ca2+ rise within a narrow window of concentration prevents functional injury of mitochondria exposed to hypoxia ⁄reoxygenation by increasing antioxidative defence pdf

... rates of respir-ation (A) , RCR (B) and the ratio of the rates of state 3 respirationbefore and after the addition of NADH and cytochrome c (C) arepresented. The rate of state 3 respiration of ... mitochondrial damage. Our data demonstrate thathypoxia ⁄ reoxygenation and extramitochondrial Ca2+cause functional damage of isolated rat liver mitochon-dria. Essential steps involved in the cascade ... Long-lasting ischaemia in liverwas shown to induce cytochrome c release and necrosis,whereas short ischaemia with reperfusion results in the release of cytochrome c and apoptosis [5]. A further...

Tài liệu Báo cáo khoa học: Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different ﬂanking bases preference pptx

... an extra effector in which the activation domain of viral protein 16 was fused to the yeast GAL4 DNA binding domain (DBD) and then coexpressed with the AtERF4 effector was used to test the in ... AtERF family is further divided into varioussubgroups according to the homology of ERFdomains [5,6].An ERF domain consists of a three-stranded anti-parallel b-sheet and an a- helix, packed approximatelyparallel ... either the GCC or the DRE motif. The most remarkable feature arising from the competition binding assay was the consistency of the binding preference of the AtERFs with the EMSAanalysis. The...

Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx

... Com-mittee of the University of Balamand and complied with the principles of laboratory animal care.Chemicals and reagentsMuramyl peptides (MDP and MB) used in this work werekindly provided by ISTAC-SA ... immune and ⁄ or inﬂammatory responses [14,15]. Infected perito-neal macrophages of UCP2) ⁄ )mice are resistant to infection by the intracellular parasite Toxoplasma gon-dii through a mechanism ... macrophages,manipulation of cytokine release and restoration of haematopoiesis [18–20]. The parent molecule of thisfamily is muramyl dipeptide (MDP), which has beenreported as the minimal adjuvant-active structure...

Báo cáo khoa học: Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase a is required for Src activation doc

... extra-cellular ligands [4,5]. Besides the extracellular domain, the majority of the RPTPs present another interestingfeature: tandem catalytic domains. The membrane-proximal catalytic domain (D1) and the ... January2010)doi:10.1111/j.1742-4658.2010.07584.xReceptor protein-tyrosine phosphatase a (RPTPa) is a transmembrane pro-tein with tandem cytoplasmic phosphatase domains. Most of the catalyticactivity is contained by the membrane-proximal catalytic ... proteins and pNPP as a substrate. The phosphatase activity of D2-R554H was compared with that of wild-type(WT)-D2 and with that of D2-C723S, a catalyticallydead mutant with a mutation in the essential...

Báo cáo khoa học " ĐẶC ĐIỂM KỸ THUẬT TRONG THI CÔNG GIÀN MÁI KẾT CẤU THÉP KHẨU ĐỘ LỚN NHÀ THI ĐẤU THỂ DỤC THỂ THAO THÀNH PHỐ ĐÀ NẴNG " potx

... s a ch a các mặt bích hở 80mm ÷ 90mm là thay đoạn ống bị hụt bằng đoạn ống dài hơn (khi nối ống có lót ống bên trong). Giàn giáo phục vụ s a ch a lắp dựng ở r a sàn thao tác. Theo đề xuất c a ... phải v a đảm bảo mức chuyển vị nằm trong phạm vi tính to n thiết kế đ a ra là 100mm, v a đảm bảo an to n tuyệt đối cho công trình. Ban đầu nhà thầu đề xuất biện pháp chế tạo 32 cơ cấu visme nâng ... tiến biện pháp di dời chóp trung tâm, chuyển sang dùng hệ thống kích thủy lực, v a hiệu quả, v a an to n hơn; - Tính to n một cách khoa học để tránh sai lầm khi xác định hành trình di dời chóp...

7
1,668
31

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: histone H3 lysine 4 methyltransferases from yeast to human pptx

... it [1,2]. The corehistone particle comprises a tetramer of histones H3 and H4 and dimers of histones H 2A and H2B [2]. Each of these histones has a structured core globular domain and an unstructured ... methyltransferases and others may work efﬁciently in treating diseasescaused by epigenetic misregulations. In fact, a combi-natorial therapy targeting histone deacetylases and DNA methyltransferases ... prostate can-cer. The implication of HMTs and demethylases incancer and human diseases has led to the idea of utiliz-ing them as therapeutic targets. In fact, biguanide and bisguanidine polyamine...

Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... agree reasonably with DpK a ¼ 1.2 predicted on the basis of pK a values of phosphate and the cofactor 5¢-phosphate in a modelcompound. These data are consistent with a pH-depend-ent mechanism ... formation and a ppearance of thermostabilization by phosphate has been examined. The role of the cofactor5¢-phosphate group in the induction of stability and stabil-ization of the CcStP dimer has ... which the cofactor phosphate must beprotonated so that catalysis to a- glucan synthesis occurs[1,28,29]. The pH proﬁles of log kphofor wild-type and PL-CcStP decreased above an apparent pK a value...

Báo cáo khoa học: Staying on message: design principles for controlling nonspeciﬁc responses to siRNA pdf

... recognizes the duplex ratherthan the component single strands. An additionaladvantage to modifying the backbone chemistry of the sense strand is that by impairing the incorporation of this strand into ... [13,14]. OAS1 is activated by binding to dsRNA and induces sequence-independent degrada-tion of viral and cellular single-stranded RNA(ssRNA) by activating RNaseL [15]. OAS1 also playsan important ... (2009) The promises and pit-falls of RNA-interference-based therapeutics. Nature457, 426–433.5 Kleinman ME, Yamada K, Takeda A, ChandrasekaranV, Nozaki M, Bafﬁ JZ, Albuquerque RJ, Yamasaki S,Itaya...

Xem thêm