... variety of celltypes, including hepatocytes, to long-chain saturatedfatty acids (SFAs) led to increased expression of proin-flammatory cytokines, inhibition of insulin signaling, induction of endoplasmic ... the initiation of ER stress- induced apoptosis. Oleate was unable to inducethe expression of the proapoptotic protein CHOP, and its coadministration with stearate inhibited CHOPexpression induced ... resuspended in NaCl ⁄ Pifor 20 min at 37 ° C, and stained with Hoechst 33342(30 min, 37 °C). Annexin V–fluorescein isothiocyanate(FITC) (BD Pharmigen, San Diego, CA, USA) binding and PI staining were...