Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx
Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx
... compilation ª 2011 FEBS Catalytic mechanism of the primary human prostaglandin F2a synthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) Nanae Nagata1, ... as the (a ⁄ b)8-barrel fold [ 3–6 ]. The nucleotidecofactor binds in an extended conformation at the top of the a ⁄ b-barrel, with the nicotinamide ring projectingdown into the center of the ... PGF2a in the absence of NADPH or NADP+and in the presence of NADPH,respectively.AbbreviationsAKR, aldo-keto reductase; PG, prostaglandin; PGDS, prostaglandin D synthase; PGFS, prostaglandin...
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Tài liệu Báo cáo khoa học: Catalytic mechanism of SGAP, a double-zinc aminopeptidase from Streptomyces griseus pdf
... exact binding mode of the hydroxide to the metal ions, the proton pathway in catalysis and the specific involvement of the catalytic residues in the enzymatic reaction. The two zinc ions in the active ... protein residues near the active site, whereas in AAP the free amine interacts with the second zinc ion (Zn2) [2 4–2 8].Open issues regarding the catalytic mechanism underlying SGAP include the ... presumably transferred by the aci-dic residue (Glu131) to the amine group of the leaving peptide bringing to the break-down of the intermediate. Dashed lines indi-cate stabilizing interactions and...
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Báo cáo khoa học: Targeting mechanism of the retinoblastoma tumor suppressor by a prototypical viral oncoprotein Structural modularity, intrinsic disorder and phosphorylation of human papillomavirus E7 doc
... equilibrium, increasing the PII propen-sity of the E7 CR2 region. The E7 C-terminal domain binds independentlyto RbAB The increased affinity of the full-length E7 proteincompared with the E7 N-terminal ... specificity of the E7C–RbAB interac-tion. A peptide containing the CR2 region of E7 didnot compete with E7C binding, indicating that E7Cdoes not bind to the RbAB domain at the LxCxE-binding cleft ... 90% of the binding energy is determined by the LxCxEmotif, with an additional binding determinant (1.0 kcalÆmol)1) located in the C-terminal domain of E7, establishing a dual-contact mode. The stoichiometry...
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Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
... present in the spleen of these mice. For the determination of the number of PMM56 bacteria present in the spleen of these four mice, we thus chose avalue of 50 CFU per spleen. The average number of ... determined by calculating the ratio of mutant to wild-type bacteria after correcting for the ratio of these strains in the inoculum. It appearedthat, 21 days after infection, the ratio of PMM56 ... roles of the various members of the DIM family,e.g. DIM A and DIM B, in these functions have neverbeen investigated. The recent developments in ourunderstanding of the biosynthetic pathway of...
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Báo cáo khoa học: Spectroscopic investigation of the reaction mechanism of CopB-B, the catalytic fragment from an archaeal thermophilic ATP-driven heavy metal transporter potx
... significant shifts of the a, b and c absorbance bands, resulting from stronginteractions of phosphate groups with amino acid sidechains lining the nucleotide binding site of the protein[26,3 1–3 4]. It ... production of inorganicphosphate was observed in the absence of Mg2+, whichindicates that Mg-ATP is the substrate of CopB-B.Furthermore, the ATPase activity increased in the temperature interval ... determined from the slope of the linear plot of the apparent binding constants Kappdissand [L0]. With the ligand ATP, a binding constantKligdiss of 10 lm was obtained (Fig. 2C). The non-hydro-lysable...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... excluded all residuesinvolved in the binding of the metal cofactor.ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in the range1 0–2 00. ApeSOD and ... metal-binding siteat the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the C-terminal domain ... focuson the coordination of the metal cofactor in the activesite as well as the changes it experiences in response todifferent metal cofactors. Finally, by comparing Ape-SOD with the SOD from the...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... named according to the residue in the mid-point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- and 60-loops border the S2¢ pocket of the proteinase. The observed ... formation of the catalytic machinery and structures required for binding of the main chain of the substrate peptide and properpositioning of the scissile bond with respect to the cata-lytic serine. ... cystein-rich(SRCR) domain, which was rigidly bound to the back of the proteinase domain in the crevice between the C-terminal helix, the 204-loop and the 126-loop [7]. The C-terminus of hepsin...
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Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafficking of human acetylcholinesterase and butyrylcholinesterase potx
... counterparts, in agreement with the higher mass of BChE subunits [37]. This indicates apredominant in uence of the catalytic domain onoligomerization.Role of the C-terminal cysteineMutation of the ... modified,suggesting that degradation was increased by suppres-sion of the cysteine.Thus, it appears that the effect of a C-terminalcysteine on the trafficking of cholinesterase in the secretory ... fragment of ColQ [17]. However, the catalytic domains are also involved in quaternaryinteractions that certainly participate in the formationand stability of these oligomers. In particular, the...
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Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
... Subsequently, the GST–PP2Aca:His–TIPRL complex was incubated withHis–a4 and the GST–PP2Aca:His–a4 complex wasincubated with His–TIPRL. Binding of His–TIPRL to the previously formed GST–PP2Aca:His–a4 ... occlusion of the active site, in vitro binding assayswere performed in the presence of the PP2Ac inhibi-tor okadaic acid. These assays showed that binding of His–TIPRL or His–a4 to GST–PP2Ac was ... respectively; I: input; B: bound. (B) The experiment shown in (A) wasrepeated using a C-terminal deletion of a4(a 4D22 2) instead of full-length protein to show that only the PP2Ac-interacting domain of a4issufficient...
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Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot
... in inhibitor binding and suggest the proteinsuse a similar catalytic mechanism. These findingsunderline the importance of the evolutionarily con-served phosphodiesterase activity of 2H proteins ... regrowthupon the optic nerve crush, and also expressed in the germinal neuroepithelium of retina, which generatesnew neurons throughout the lifespan of the fish [11]. The cloning of the RICH proteins ... fragmentsgroup together in the vicinity of the N-terminus. Thishelical patch is the most basic part of the moleculeand a potential interaction surface for the precedingacidic N-terminal domain.Structural...
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