... absent in the cyto-
plasmic fraction of the DfpoF mutant.
This observation prompted investigation into the
function of FpoF in more detail. The corresponding
gene, mm0627, was expressed in E. ... in favour of this hypothesis
came from the finding that the rate of F
420
H
2
-depen-
dent heterodisulfide reduction in cell lysates of the
DfpoA-O mutant or the D...
... contained a 30 bp insertion in the
ORF, beginning at nucleotide 516. This resulted in
insertion of a 10 amino acid segment (TREIDYPETI)
and one substitution (Ser fi Gly) at the C-terminal
end of the ... ortho-
log of the product of this gene. In the clade including
Spa
¨
tzle-1, the branch lengths are noticeably longer and
the bootstrap values are lower than in t...
... The
protein part that holds two of the Fe-S clusters in the small subunit of
the D. gigas enzyme is missing in HoxY of the SH, suggesting an
exposure of part of the hydrophobic surface of the HoxH ... changes of the
Ni-Fe site. The Fe-S clusters in the HoxFUY subunits
and the flavin in the HoxF subunit are all considered to
be functional in the...
... argues against a role for a3inthe
formation of native MII binding sites, but may reflect the
scarcity of these sites in the investigated brain tissues and/or
the formation of low affinity binding sites ... microdomains within the same binding
site [35]. The proline residue in position 6, which is
conserved in all other a-conotoxins, appears to be the
major determinant o...
... tripalmitin as standards. The scattering
patterns were collected in 15 frames of 1 min to verify
the absence of radiation damage. The experimental data
was normalized to the intensity of the incident ... enol form of indolepyruvate (Fig. 1). In
the course of the establishment of the equilibrium % 85% of
the enol form is converted into the ketone (half-tim...
... successful metabolic engineering
of freezing tolerance in plants should include such an
analysis of the dynamics of metabolism to gain com-
prehensive information about the effects of individual
overexpression ... simulta-
nous reduction in hexose concentration, particularly
during the initial period of cold exposure. Using RNA
interference-mediated inhibition of the d...
... N7 (Fig. 1C). The 2¢OH of the ribose is hydro-
gen bonded to the main chain and the side chain of
Asp142 (Fig. 1C). Both hydroxyl groups of the ribose
are involved in coordinating a putative ... solutions. The ligand
bound was interpreted as GMP because the N2 of the
base makes a hydrogen bond to a main chain car-
bonyl. Binding of a xanthine base from XMP in th...
... all-alpha domain, STAT
protein DNA-binding domain and SH2 domain of
stat6, and the zinc-finger domain, leucine-zipper
domain and fork-head domain of foxp3. In addition,
for stat6 and foxp3, there were ... adjacent DNA-binding sites,
a coiled-coil STAT protein all-alpha domain, which is
implicated in other protein–protein interactions, a
STAT protein DNA-binding domain and an SH2
doma...
... strains [3]. Pathogenic
Yersiniae are typically taken up through ingestion and
first reach the intestine. The Yersinia surface protein
invasin binds to b1 integrins on the apical surface of
M ... In
addition to actin, other host cell proteins have been
shown to bind to the virulence factor YpkA, including
OTUB1 [13]. In order to investigate the role of
OTUB1 in Yersinia inv...
... number of these modifications is low, the density of
modifications is much the same as in the rest of the lin-
ker histones. It is the shortness of the N-terminal tails
Table 1. Alignment of chicken, ... the triphosphate form being incorpo-
rated into DNA in place of the natural base cytosine
[12]. The use of such analogues results in the global
depletion...