Báo cáo khoa học: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosaminidase pot
Báo cáo khoa học: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosaminidase pot
... Department of Structure and Function of Proteins, Institute of Nanobiology and Structural Biology of GCRC, Academy of Sciences of theCzech Republic and Faculty of Sciences of the University of South ... hydrolysis of N-formyl, N-glycolyl and N-propionyl derivatives of the standard N-acetylsubstrate) than A. oryzae (2–5% hydrolysis).The cloning and sequencing of PoHex and analysis of the corresponding ... carbohydrates (left panel), protein (middle panel) and for enzymatic activity (right panel). Lane 1,PoHex; lane 2, PoHex plus Endo H; lane 3, PoHex plus a-mannosidase; lane 4, Endo H; lane 5, a-mannosidase....
- 16
- 429
- 0
Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx
... s)1 and Km of 0.148 mmtoward shikimate, kcat of 7.1 s)1 and Km of 0.182 mm toward NADP, kcat of 5.2 s)1 and Km of 2.9 mm toward NAD. The optimum pH of theenzyme activity is between 8.0 and ... catalytic proper-ties of HpSDH and the effects of pH and temperatureon HpSDH. The results show that HpSDH has a kcat of 7.7 ± 0.9 s)1, Km of 0.148 ± 0.028 mm and kcat⁄ Km of 5.2 · 104m)1Æs)1toward ... 4, and 5 display noncompetitive inhibi-tion mode, and compounds 2 and 3 show competitive inhibition mode withrespect to NADP. Antibacterial assays demonstrate that compounds 1, 2, and 5 can...
- 11
- 529
- 0
Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... human and pufferfish (torafugu and spotted greenpufferfish) IL-10 sequences. Pufferfish and carp IL-10genes, clustered together and distant from IL-20 and IL-24, as recently determined from analysis ... controlfor the quantity and quality of cDNA.Semiquantitative analysis of RT–PCR products. Suspen-sions of Carp head kidney and liver cells were treated with10 lgÆmL)1LPS for 1, 3 and 6 h, individually, ... carp IL-10 and b-actin genes were amplified usinga range (21–30) of PCR cycles. Following this procedure, an optimal number of PCR cycles (24 for IL-10 and 21 for b-actin) was determined and subsequently...
- 8
- 584
- 0
Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt
... [23,30], and the bands at1172, 1085, and 1038 cm)1assigned to glucose ringvibrations [31]. As no additional bands in the range of theamide vibrations centered at 1650 and 1550 cm)1can beobserved, ... crystallinephase transition for phosphatidylcholine, MfGl-II, and LPS Re.Fig. 3. Infrared spectrum in the spectral range 1800–900 cm)1(A) and in the range of the negatively charged phosphate band mas(PO2–) ... 1300–1190 cm)1(B)ofhydratedPC,MfGl-II,andLPSRe.3274 K. Brandenburg et al. (Eur. J. Biochem. 270) Ó FEBS 2003lipopolysaccharides can be excluded. The band contour of mas(PO2–) was analyzed after...
- 9
- 665
- 1
Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... GLUT3(59–60%) and GLUT4 (55–59%) proteins, and moderateFig. 1. Organization of the gcGLUT gene. (A)The 12 exons of the gcGLUT gene are shownas boxes. Filled and open boxes indicatetranslated and untranslated ... identification of a hypoxia-responsive glucose transpor-ter gene (gcGLUT) and the corresponding full-length cDNAfrom the grass carp. The gene spans 11 kb of genomicsequence and consists of 12 exons and ... exons and 11 introns, and an open reading frame (ORF) of 1599 bp encoding a poly-peptide of 533 amino acids, with a predicted molecular mass of 57 kDa and a pI of 8.34.BLASTXanalysis showed thatthe...
- 8
- 465
- 0
Báo cáo khoa học: Identification and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc
... genomic organization to the humanIL-18 gene, consisting of six exons and five introns. TheFig. 2. Comparison of the genomic organization of IL-18 and IL-1b genes in human, rainbow trout and the ... twice with an equal volume of phenol/chloroform (24 : 1, v/v) and once with chloroform. Genomic DNA was precipitatedwith two volumes of cold ethanol, washed with 70% (v/v)ethanol, and dissolved ... of mRNA were quantified by densitometricscanning of the ethidium bromide stained gels, using an Ultra Violet Products Ltd gel imaging system and UVPGELWORKS IDadvanced software, and expressed...
- 11
- 426
- 0
Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
... structure[19] give evidence of polar environment around ArgB263.It consists of O atoms of the main chain CO groups of LeuB387 and TrpB240, Od1atom of AsnB241 and Oc atom of SerB386 and is expected to ... R.A., van Rantwijk, F., van Langen, L.M., Wegman,M.A., Cao, L. & Janssen, M.H.A. (2001) Biocatalysis and biocatalyst in the synthesis of b-lactam antibiotics. In: Synthesis of b-Lactam Antibiotics. ... Stambolieva1 and Jose Kaneti11Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria;2Laboratory of Bioorganic Synthesis, Faculty of Biology,...
- 8
- 438
- 0
Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf
... 286 ± 0.5 K and at 11.7 T on a Bruker (Karlsruhe, Germany) Avance500spectrometer, operating at 500.13 MHz and 50.68 MHz for1H and 15N, respectively. The longitudinal (R1) and trans-verse ... helix I, Ser27 and Leu34in helix II, and Lys46 and Gln50 in the N-terminalportion of helix III. For an isotropically tumblingglobular molecule, in the absence of internal motions and with relaxation ... fragment of helix III haslower mean values for both J(0) and J(xN), and asignificantly higher mean value for J(0.87xH), whichfurther stresses the different dynamic behaviors of the N-terminal and...
- 14
- 744
- 0
Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elafin) do not depend on its protease inhibitory function pptx
... nature of the molecule, as assessedfrom the salt and heparin dependence of the antimicro-bial and antifungal effects.ResultsAntimicrobial effects of recombinant wild-typetrappin-2 and trappin-2 ... PAGE gel and analyzed by western blotting using polyclonalanti-trappin-2 sera. The molecular masses of the protein standardsare shown on the left.Antibacterial and antifungal activities of trappin-2 ... (inhibitor of serine and cysteine proteases) ontrappin-2 degradation by C. albicans proteases (lanes 2–6). Trappin-2 and elafin controls are shown in lanes 1 and 7, respectively.Antibacterial and antifungal...
- 13
- 610
- 0
Tài liệu Báo cáo khoa học: Receptor association and tyrosine phosphorylation of S6 kinases pdf
... Stephens7, Andrew West7 and Ivan T. Gout2,31 Ludwig Institute for Cancer Research, London, UK2 Department of Biochemistry and Molecular Biology, University College London, UK3 The Institute of Molecular ... domains and have a similar mode of activation [1].There are two isoforms of S6 kinase, S6K1 and 2.Both have highly homologous kinase and kinaseextension domains flanked by the less conservedN- and ... 30, 60 and 180 min of serum-stimulation. Asshown in Fig. 2B, both variants of S6K1 (p70 and p85) are phosphorylated on tyrosine in an induciblemanner. Interestingly, the phosphorylation of the...
- 14
- 630
- 0
Từ khóa: Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)MÔN TRUYỀN THÔNG MARKETING TÍCH HỢP