... detected, indicating that calpainhad undergone a transition from the low to the highaffinity mAb-binding form. Interestingly, these results obtained in vivo were almost superimposable on thoseobtained ... only indicate that calpastatin inducesa pronounced Ca2+-independent change in calpainconformation, but also provide strong support for pre-vious observations indicating that calpain and calpast-atin ... the intensity of calpain staining increased 7–8-fold, indicating that the protease was now mainlypresent in the high-affinity form. In these conditions, although the highest amount ofcalpain...