... pSIB1-Nd, pSIB1-Cd, and pSIB1-a3+Cd for over-production of a His-tagged form of the N-domain of SIB1FKBP22 with entire a3 helix (N-domain+), C-domain with-out a3 helix (C-domain–), and C-domain ... proteolysis of L. pneumophila MIP allows the separation of their N- and C-domains such that the C-domain contains the C-terminal half of the a3helix [23,24]. In addition, the C-domain of E. coliFkpA ... peaks of heat capacityobserved in thermal unfolding of SIB1 FKBP22* rep-resent unfoldings of its N- and C-domains. In thisthermal unfolding process, the C- and N-domainsunfold at lower and...