... mutations in the AUH gene. This indi-
cated that AUH, in addition to its RNA binding func-
tion, must play an important role in leucine catabolism.
AUH was overproduced in E. coli and characterized
by ... of leucine catabolism at the protein
and DNA levels and developed a novel assay for
enzyme analysis in a diagnostic setting. The human
AUH protein was first recognized by...
... digest-
ible protein substrate, and finally calpastatin.
The finding that calpastatin was the most efficient
ligand at promoting calpain transition, detected as an
increase in mAb that bound to calpain, is ... the intensity of calpain staining increased 7–
8-fold, indicating that the protease was now mainly
present in the high-affinity form.
In these conditions, although the highest amoun...
... findings, our research provides new insights into
the role of exon 9 in the regulation of apoptosis and
invasion in liver cell lines.
A
B
Fig. 7. Effects of occludin shRNA on apoptosis and invasiveness. ... level
increased in Occ
WT
-overexpressing cells, and that
2-APB inhibited the effects of Occ
WT
. Therefore,
the increase in intracellular calcium may explain the
induction...
... places, but in particular, stefin B has a number
of histidines in the C-terminus (box in Fig. 1) at sites:
92, 75, 66 and 58. As histidine residues are central to
copper binding in many proteins they ... competitor to nonspecific protein copper interactions.
A control ITC experiment of titrating stefin B protein with
glycine alone was performed and no binding was observed.
In our h...
... P
i
released min
)1
Æmg
)1
of protein.
Enzyme kinetic study
The K
m
(Michaelis constant) and V
max
(maximum velocity)
values were determined by incubating the diluted recombin-
ant proteins in the standard ... at 26 000 g for 30 min
at 4 °C. The supernatants containing recombinant proteins
of AsPPase were purified using ProBond
TM
resin (Invitro-
gen) under nondenaturing conditions and s...
... a protein
containing a single ankyrin repeat that interacts with
HIV-1 viral protein R (Vpr) and we designated this
protein as Vpr-binding ankyrin repeat protein
(VBARP). This interaction was ... aprotinin, and protease inhibitors leupep-
tin, chymostatin, and pepstatin (1 lgÆmL
)1
; Sigma) as des-
cribed [14] for 15 min in ice. Cell lysates were centrifuged
at 22 000 g for 10 min at...
... (Jacobson and Aaker, 1993; Ali and Hwang, 2000).
In addition, instead of using NI in our regression model, we have also used ∆NI, change
in earnings (current earnings minus lagged earnings all ... complex earnings-generating
process, may have weaker earnings-return relations than firms operating in a single
industry.
15
Finally, we include firm size as a control for other missi...
... (4NPGlc), incubating for 30 min at 37 °C, and
measuring the rel ease of 4NP.
Protein assays and protein sequencing
Total protein in crude and semipuri®ed samples was
estimated using the Pierce Protein ... ncreasing
D t o 2 0% (10 min), 50% ( 15 min), 100% (5 min), held at
100% (5 min); cyanodin glycosides; 5% B/95% A initial
(5 min), increasing B to 20% (10 m in) , 90% (10 min), held...
... Earth
and Beyond
A Decadal Research Strategy in
Solar and Space Physics
Solar and Space Physics Survey Committee
Committee on Solar and Space Physics
Space Studies Board
Division on Engineering and ... future directions of U.S. ground- and
space- based programs in solar and space physics research. The NRC’s
Space Studies Board and its Committ...