. expansins and a small (F45) family of fungal endoglucanases. However, hydrolytic activity (exo and endo type) of expansins on polysaccharides has neverbeen detected, and F45 hydrolases fail to stimulate. ligationinto SnaBI-digested vector pPIC9 (P. pastoris ExpressionKit; Invitrogen, San Diego, CA, USA), directly after thea-mating factor leader sequence, which mediates export ofrPhl p 1 into the. Promega, Madison, WI, USA), and sequenced. To construct the mutated form, fragments werereleased using the restriction enzymes SmaIandSnaBI orEcoRI and SnaBI, respectively, and both ligated in pBS,which...