... 1B shows the patternseen after the development of the blot. With increasein time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... presence of 100 lM MPTP and 50 lM dopamine (F), in the presence of 200 lM MPTP and 50 lM dopamine (G), in the presence of 100 lM MPP+ and 50 lM dopamine (H) and in the presence of 200 lM MPP+ and ... a-synucleinwas because of MPTP alone and not because of its con-version to MPP+, RP-HPLC of the samples was car-ried out. The incubated samples (a-synuclein alone and in the presence of 100 and 200 lm...