... restriction enzymes were suppliedby New England Biolabs (Hitchin, Herts, UK). LMW-SDSmarker kit and HMW native marker kit were purchasedfrom GE Healthcare (Uppsala, Sweden). Phytate (sodiumsalt), ... purified PhyH-DI. (B) Non-denaturing gradient PAGE ofPhyH. Lane M, native high molecular weight markers; lane 1, nativePhyH stained with Coomassie Brilliant Blue R250.0204060801001203456789101112pH ... PhyH-DII. (A)SDS ⁄ PAGE analysis of purified PhyH, PhyH-DI and PhyH-DII. LaneM, molecular weight markers; lane 1, culture supernatant of PhyH;lane 2, culture supernatant of PhyH-DII; lane 3, culture...