... 500 (m ⁄ m) for 4 h at 37 °C. Before MS analysis, allsamples were stored at )30 °C.MALDI-TOF⁄TOF MS analysis Analysis of the digests was conducted by offline nano-HPLC ⁄ MALDI-TOF ⁄ TOF MS, ... explained by thefact that the GYGPG motif is preceded by eightamino acids that could facilitate the adoption of anactive conformation and its stabilization. This hasalso been suggested by Moroy ... described:YTTGKLPYGYGPGG (residues 221-234, released by MMP-7, MMP-9, and MMP-12), YGARPGVGVG-GIP (residues 383–395, released by MMP-12), andTable 4. (continued)Fig. 5. Zymography analysis of pro-MMP-2secretion....