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Báo cáo Y học: Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase b subunit in the energy coupling mechanism of the Na+ pump ppt

Báo cáo Y học: Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase b subunit in the energy coupling mechanism of the Na+ pump ppt

Báo cáo Y học: Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase b subunit in the energy coupling mechanism of the Na+ pump ppt

... Role < /b> of < /b> conserved < /b> residues < /b> within < /b> helices < /b> IV < /b> and < /b> VIII < /b> of < /b> the < /b> oxaloacetate < /b> decarboxylase < /b> b subunit in the < /b> energy coupling mechanism of < /b> the < /b> Na+ pump Markus Schmid, Thomas Vorburger, Klaas ... with the < /b> participation of < /b> the < /b> a and < /b> the < /b> c subunit (Eqn 1). Subsequently, the< /b> carboxybiotin moiety switches to the < /b> decarboxylase < /b> site on the < /b> b subunit and < /b> is decarboxylated under consumption of < /b> ... hydroxyl side chain of< /b> tyrosine 229. This displacement may be facilitated involving by R389 through lowering the < /b> pK of < /b> the < /b> tyrosine hydroxyl group. The < /b> proton isdelivered to the < /b> carboxybiotin and...
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Báo cáo khoa học: Plasmodium falciparum merozoite surface protein 1 Glycosylation and localization to low-density, detergent-resistant membranes in the parasitized erythrocyte pdf

Báo cáo khoa học: Plasmodium falciparum merozoite surface protein 1 Glycosylation and localization to low-density, detergent-resistant membranes in the parasitized erythrocyte pdf

... detectable only as a single 19-kDa band. The< /b> only strong [3H]GlcN-labelledbandinthetotalextractmatching the < /b> Western blotted material was a 17-kDa band. The < /b> majority of < /b> the < /b> [3H]GlcN-labelled ... werepreferentially found in the < /b> membrane network of < /b> the< /b> parasitized erythrocyte. One 100-kDa peptide bearing bothN- and < /b> C-terminal epitopes was detected by both antibodieson Western blots of < /b> the < /b> pellet and < /b> ... originally described in erythrocyte ghosts were characteristically rich in sphingo-lipids and < /b> cytoskeletal proteins spectrin, actin and < /b> band 4.1[41]. Using standard equilibrium sucrose density...
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Báo cáo Y học: Role of critical charged residues in reduction potential modulation of ferredoxin-NADP+ reductase Differential stabilization of FAD redox forms doc

Báo cáo Y học: Role of critical charged residues in reduction potential modulation of ferredoxin-NADP+ reductase Differential stabilization of FAD redox forms doc

... experimentallyeither the < /b> Kdor the < /b> binding energies for the < /b> apoFNR:FADcomplexes in any redox state. However, as the < /b> reductionpotentials of < /b> free and < /b> bound FAD are linked to the < /b> bindingaffinities of < /b> the < /b> ... supported by the < /b> H-bond between K75 FNR and < /b> E94 Fd observed in the< /b> complex [8] (Fig. 1). Structural analysis also suggested that the < /b> E301 carboxylate might be involved in the < /b> catalysis bytransferring ... not involved in any intraproteininteraction, but is situated at the < /b> entrance of < /b> a watercavity, at the < /b> bottom of < /b> which are the < /b> pyrophosphate and< /b> the < /b> ribose from the < /b> FAD. Therefore, K75 cannot bedirectly...
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Báo cáo y học:

Báo cáo y học: " Role of Dietary Soy Protein in Obesity"

... been identified in a number of < /b> studies in soybean-sensitive patients These include include b- conglycinin, glycinin, soy vacuolar protein, Kunitz trypsin inhibitor, and < /b> other proteins [83,87-89]. ... attributed in part to an increase in circulating levels of < /b> cholecystokinin. Similarly, in rats fed a hy-percholesterolemic diet, ingestion of < /b> the < /b> alpha subunit of < /b> the < /b> soy 7S globulin (conglycinin) ... suggesting that soy protein reduces liver triglycerides or fat by partly inhibiting hepatic fatty acid synthesis in the < /b> liver. ACC, the < /b> rate-limiting en-zyme that catalyzes the < /b> carboxylation of...
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Tài liệu Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum ppt

Tài liệu Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum ppt

... of < /b> the < /b> protein surrounding the < /b> copperligand H92. R93 has been shown to be essential for the< /b> interaction of < /b> plastocyanin with photosystem I in Anabaena[15], and < /b> is highly conserved < /b> in cyanobacterial ... repulsion. The < /b> attractionbetween wild-type plastocyanin and < /b> Cyt f could be virtuallyabolished by neutralizing a single positive charge (K53A),whereas the < /b> repulsion between wild-type plastocyanin and< /b> Fig. ... thatonlychargesoftheproteinattheactivesitearerelevant ,and< /b> these can be represented by a disc of < /b> fixed radius and< /b> uniform charge. The < /b> same analysis has been used for the< /b> interaction between Cyt f and...
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Tài liệu Báo cáo Y học: Role of sulfoquinovosyl diacylglycerol for the maintenance of photosystem II in Chlamydomonas reinhardtii ppt

Tài liệu Báo cáo Y học: Role of sulfoquinovosyl diacylglycerol for the maintenance of photosystem II in Chlamydomonas reinhardtii ppt

... beingsprayed with primulin.Table 1. Photosystem II activity of < /b> C. reinhardtii hf-2 in which deriva-tives of < /b> SQDG and < /b> PtdG were incorporated by incubation for 48 h. The< /b> values obtained in the < /b> ... decrease of < /b> PSII activity evenwithout the < /b> conformational change in protein complex.Diuron inhibits PSII activity by binding to the < /b> relativelyhydrophobic Q B pocket, which is the < /b> exit of < /b> the < /b> electronflow ... the< /b> decrease of < /b> PSII activity in hf-2bythelackofspecificbinding of < /b> SQDG to the < /b> PSII complex. Alternatively, the< /b> change of < /b> the < /b> lipophilic surrounding at Q B site of < /b> the < /b> PSIIcomplex might cause the < /b> decrease...
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Báo cáo Y học: Role of three isoforms of phospholipase A2 in capacitative calcium influx in human T-cells pot

Báo cáo Y học: Role of three isoforms of phospholipase A2 in capacitative calcium influx in human T-cells pot

... the < /b> inhibitors of < /b> type IB and < /b> type V, and < /b> BEL, an inhibitor of < /b> type VI,inhibited significantly the < /b> TG-induced [3H]AA release. The< /b> type IV-< /b> PLA2does not seem to play a role < /b> in the < /b> release of< /b> AA ... acid and < /b> 4-bromo phenacyl bromide (BPB)which are the < /b> inhibitors of < /b> sPLA2, i.e. type IB/typ V.Arachidonyl trifluoromethyl ketone (AACOCF3) and< /b> bromoenol lactone (BEL) are the < /b> respective inhibitors ... inhibitors of< /b> type IV < /b> and < /b> type VI PLA2. Methyl-arachodonyl fluoro-phosphonate (MAF) inhibits type IV < /b> and < /b> type VI PLA2with the < /b> same selectivity. We observed that aristolochic acid,BPB and < /b> BEL, but...
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Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx

Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx

... properties in the < /b> free form of < /b> Y2< /b> 38S are different. The < /b> binding of < /b> the < /b> carboxylic acidcompetitive inhibitors and < /b> the < /b> substrateD-alanine arechanged only slightly, suggesting that the < /b> overall substratebinding ... coefficients for the < /b> mutant DAAO enzymeswere determined by measuring the < /b> change in absorbanceafter release of < /b> the < /b> flavin. The < /b> enzymes were heatdenatured by boiling for 5 min in the < /b> dark (an extinctioncoefficient ... toward the< /b> hydrophobic binding pocket of < /b> the < /b> active site (see Fig. 1A).R285 has been mutated to lysine, glutamine, aspartate, and< /b> alanine [10]. The < /b> perturbation of < /b> the < /b> active site in the < /b> R285mutants...
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Báo cáo Y học: Role of histidine 42 in ascorbate peroxidase Kinetic analysis of the H42A and H42E variants pptx

Báo cáo Y học: Role of histidine 42 in ascorbate peroxidase Kinetic analysis of the H42A and H42E variants pptx

... mM)1Æcm)1[42]. Activities were determined by dividing the < /b> change in absorbance by the < /b> absorption coefficient of < /b> the < /b> substrate.Values for kcatwere calculated by dividing the < /b> maximumrate of < /b> activity (lM)1Æs)1) ... mutations and,< /b> together with His42, may be in uen-tial in defining the < /b> stability of < /b> Compound II. The < /b> replacement of < /b> the < /b> distal histidine residue by alanineor glutamic acid clearly has a profound in uence ... histidineresidue stabilizes Compound II through a hydrogen-bond-ing structure involving the < /b> Ne of < /b> His42 and < /b> the < /b> ferryloxygen. However, although hydrogen-bonding interactions of < /b> this kind have been...
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Báo cáo Y học: A functionally conserved member of the FTZ-F1 nuclear receptor family from Schistosoma mansoni doc

Báo cáo Y học: A functionally conserved member of the FTZ-F1 nuclear receptor family from Schistosoma mansoni doc

... 18 and < /b> 19). Lanes 4 and < /b> 5show binding to SFRE in the < /b> presence of < /b> antibody against SmFTZ-F1 and < /b> preimmune serum, respectively. A band corresponding to non-specific binding is indicated by the < /b> ... kb in length. Southern blotting (notshown) indicates that there is only one copy of < /b> the < /b> gene and< /b> this is supported by the < /b> presence of < /b> only three positiveclones in the < /b> S. mansoni BAC library ... out by removing aliquots of< /b> the < /b> polymerase reaction every four cycles starting at the< /b> eighth cycle, dot-blotting the < /b> samples on to a charged nylonmembrane and < /b> hybridizing exactly as previously...
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