Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx
Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx
... Purification and biochemical characterization of some of the properties of recombinant human kynureninase Harold A. Walsh and Nigel P. BottingSchool of Chemistry, University of St Andrews, ... regulation of enzyme activity in vivo and consequent channeling of substrate 3-hydroxykynurenine down the tryptophan–kynurenine metabolic pathway. The purification of recombinant human kynureninase to ... pathophysiology of human diseaseshas always been hampered by the availability of human tissue, aesthetics, and ethical considerations. The principleaim of this study was to express a clone of human kynureninase...
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Báo cáo Y học: Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod pdf
... (saponification) of the N-glycans yielded glycans devoid of additional acetylgroups, an indication that these were O-acetyl substituents[15]. The MALDI-TOF spectrum of the de-O-acetylatedglycans reveals the ... belonging to class 3 of the cystatinsuperfamily. They are single-chain proteins composed of anN-terminal heavy chain, the bradykinin moiety and aC-terminal light chain. The heavy chain and light chain ... with the heavy chain of human kininogen (Fig. 3), verifying the identity of these novel kininogens. The highly conservedcystatin sequence QVVAG was also found among the codsequences. The complete...
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Báo cáo Y học: Purification and characterization of three galactose specific lectins from Mulberry seeds (Morus sp.) ppt
... galactose.Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as the standard. The presence of sugar in the protein was detected by periodic ... In the other side of the tank, a light source wasplaced in order to attract the nauplii. Two days were allowedfor the hatching of all the eggs and sufficient maturation of the nauplii for the ... by the Lowry method. The neutral sugar contents of the lectins, MSL-1, MSL-2, and MSL-3 were found to be 5.7, 5.4 and 4.5%, respectively. The sugar composition of the lectins as identified by...
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Báo cáo Y học: Purification and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx
... VanX- and VanY-type enzymesindicated that all of these enzymes contained the same Zn2+binding motif. VanX homologues present in the cyanobac-terium Synechocystis strain PCC6803, and the glycopeptideantibiotic ... aD-Ala-D-Ser standard curve. Fordetermination of the rate of hydrolysis of UDP-MurNAc-pentapeptide[Ala], His6–VanXYCwas used at a concentra-tion of 2.25 · 10)7M. The rates of hydrolysis of 2, ... parameters of D59A for hydrolysis of UDP-MurNAc-pentapeptide[Ala]. However, its activitywas comparable with that of D59S and His6–VanXYC .The rates of hydrolysis of D-Ala-D-Ala and UDP-MurNAc-pentapeptide[Ala]...
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Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx
... versatile enzymes innonaqueous solvent systems in which they catalyze the synthesis of a variety of acylglycerols and specialized estersvia transesterification. Lipases demonstrating high activityunder ... in detergents,one of the largest industrial uses of these enzymes [4].Lipase-catalyzed synthesis of structured triacylglycerolscomprised of both long and medium chain fatty acids hasbeen investigated ... biocatalyst.Keywords: lipase; LipA; Acinetobacter sp.RAG-1;protein purification; zymogram.Lipases are glycerol ester hydrolases (EC 3.1.1.3) thatcatalyze the hydrolysis of triacylglycerols to free fatty...
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Báo cáo Y học: Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans doc
... of the co o genes encoding the subunits of the CO-oxidizing:H2-evolving enzyme complex of C. hydrogenoformans. The cooMKLXUHhypA and the cooFISI gene cluster are located on contig 2340 of the ... enzyme complex from C. hydrogenoformans. Theenzymewaspurifiedfrom40gcells(wetmass). One unit of enzyme activity is defined as the amount of enzyme that catalyzes the reduction of 2 lmol methylviologen ... encodes the catalytic subunit (CooS) of the COdehydrogenase, an electron transfer protein (CooF) of the CO dehydrogenase, and proteins required for the insertion of Ni into the enzyme (CooC, T, and...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... below.AP assaysAll assays were linear with respect to time and concentration of enzyme assayed. One unit (U) of activity is defined as the amount of AP resulting in the hydrolysis of 1 lmol of substrate ... °C.Phosphatase assay AFor routine measurements of AP activity, the hydrolysis of phosphoenolpyruvate to pyruvate was coupled to the lactatedehydrogenase reaction and assayed at 25 °C by monitoring the oxidation ... absence of exogen-ous Pihad only approximately 40% of the fresh weight of the 8-day-old +Picells (approximately 63 and 25 g of cellswere obtained per 500 mL culture of 8-day-old +Pi and –Picells,...
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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
... determination of hA2aR by X-ray or electron crystallography, or infor-mation on the bound ligand conformation obtained byNMR spectroscopy, would assist in the design of subtypespeci®c compounds and ... cally to the XAC-agarose.Theophylline was removed by gel ®ltration before quanti-fying speci®c [3H]ZM241385 binding in the X AC-agaroseeluate. The value obtained was only 50±80% of the theoretical ... and amino-acid analysis. The sequence obtained, namely Lys-Ile-Glu-Glu-Gly-Lys-Leu-Val-Ile-Trp corresponds to the N-terminus of the m ature maltose-binding protein. Binding of the fusion protein to the IMAC...
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Báo cáo Y học: Puri®cation and properties of an alkaline proteinase of Fusarium culmorum doc
... speci®city of the enzymeWhen the hydrolytic activities of the proteinase weremeasured at pH 9.0 with various synthetic substrates, the results listed in Table 5 were obtained. The enzymehydrolyzed ... containers. Hence, some of the s tabilityfeatures of the enzyme may be affected by small amounts of contaminating proteins.Fig. 3. The eect of pH on the proteinase activity, measured with the azogelatin ... 799 Characterization of the proteinaseEects of pH and temperature on activity. The activities of the partially puri®ed enzyme, obtained by CMCseparation at pH 5.0, were measured by using the azogelatinassay...
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Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc
... oligomannose/hybrid glycostructure, whereas the serum enzyme and the liver enzyme belong to the groups of glycoproteins with a glycostructure of the complex type.To substantiate the notion that the TRH-DEs ... from rat and pig brain [28]. For the purification of the serum TRH-DE fractionation by poly(ethylene glycol) precipitationwas used as the first step not only to partially purify the enzyme but ... similar enzyme-chemical properties between the particulate liver enzyme and the serum enzyme were noticed [9,15].To verify the hypothesis that the serum TRH-DE is of liver origin we analyzed the TRH-DE...
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