Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot
Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot
... the Netherlands.1032 T. Happe and A. Kaminski (Eur. J. Biochem. 269) Ó FEBS 2002 Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii Thomas ... directly to the H-cluster.Protein sequencing of the enzyme and recombinantexpression of HydA in E. coliTo verify that the hydA ORF encodes t he Fe-hydrogenase of C. reinhardtii, the enzyme was ... (Fig. 3).They showed the presence of one hybridizing signal of similar intensity in different digestions, suggesting thatHydA is encoded by a single copy gene in the C. reinhardtii genome. The same...
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Báo cáo Y học: Differential regulation of telomerase activity by six telomerase subunits potx
... Alternatively, hTR and hTERTmay play a minimal catalytic activity in telomerase, while the assembly of other telomerase subunits may amplify the enzyme function. In this scenario, deletion of mTEP1 in embryonic ... of dyskerin, althoughshowing comparable inhibition of telomerase function in cells, exhibited a weaker inhibition of telomerase activity in the in vitro assay. As dyskerin is believed to be involved in ... found in proteins involved in a wide variety of cellular processesranging from signal transduction to RNA processing [28].Proteins containing WD repeats are often physicallyassociated with other...
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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
... inverselycorrelate with FAAH activity in peripheral lymphocytes[40]. The mechanism of F AAH activation b y leptin andprogesterone was further investigated. Binding of leptin to the l ong isoform of ... they do not in human neuronal cells.Therefore, the central actions of these two hormones donot seem to involve the endocannabinoid system butinstead may play a role in the effects of leptin ... anandamide(N-arachidonoylethanolamine, AEA) in human p eripheralT lymphocytes by up-regulating the activity and expres-sion of the AEA hydrolase (fatty acid amide hydrolase,FAAH) in these cells [9]. The e...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... senseor use oxygen. The intracellular concentration of hemeis maintained by the rate of its synthesis and degrada-tion [1]. Many enzymes and their regulators areresponsible for heme synthesis [1,2]. ... d-aminolevu-linic acid dehydratase, the second enzyme of the hemebiosynthetic pathway. SnPP is a competitive inhibitor of HO activity [35]. The heme content of all three celllines was significantly decreased ... andHO-2 proteins. The intensity representing HO-1 or HO-2 protein was normalized with respect to the intensity of the b-actin signal. The ratio of each normalized value to the control value in siRNA-untreated...
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Tài liệu Báo cáo Y học: Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1 potx
... the exception of the temperature-activity profile, the characteristics of the enzyme weredetermined from initial velocity measurements in the direction of the NADH-dependent dihydroxyacetonephosphate ... any amino acid [19,20]. In contrast, some NADP+binding sites have an a lanine at the position c orresponding to the third glycine residue of the conserved t rio [21]. In A. pernix Gro1P dehydrogenase, ... differently by the intracellular concentration of K+than M. thermo-autotrophicum.Kinetic analysis of Gro1Pdehydrogenase The above results show that thermophilic Gro1P dehydro-genase catalyzes the...
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Tài liệu Báo cáo Y học: Differential response of neuronal cells to a fusion protein of ciliary neurotrophic factor/soluble CNTF-receptor and leukemia inhibitory factor pot
... transport of IL-6R through the secretory pathway [32]. Secondly, we avoided the use of asynthetic polypeptide linker in order to minimize immun-ogenicity. Instead, the 16 C-terminal amino acids of CNTF-R ... differences in physiological response in neur-onal cells. The underlying molecular mechanism mightinclude the recruitment of the transducing proteins throughbinding of Hyper-CNTF and LIF to distinct ... fusion protein Hyper-CNTF does not contain asynthetic polypeptide linker, the CNTF-R and CNTF beinglinked via the flexible C-terminal portion of the CNTF-Rand the N-terminal part of CNTF [33,34]...
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Tài liệu Báo cáo Y học: Differential effects of arachidonoyl trifluoromethyl ketone on arachidonic acid release and lipid mediator biosynthesis by human neutrophils pot
... synthetase, inhibition of CoA-IT results in anincrease in free AA and a corresponding build-up of AA in triacylglycerols that can be prevented by co-incubation of cells with CoA-synthetase inhibitors ... PAF[55].Thus,whilePLA2isotypes may not be directlyinvolved in generating lyso PAF, the activity of PLA2onPtdEtn may indirectly drive the remodeling process thatgenerates the PAF precursor by providing AA acceptormolecules. ... and the radioactivity in these fractions determinedby liquid scintillation counting. Molar quantities of leuko-trienes were determined by UV spectroscopy.PAF biosynthesis The incorporation of...
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Báo cáo Y học: Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells pdf
... effectiveinhibitors of recombinant CTE-I and MTE-I. The experi-ment using inhibitors of acyl-CoA synthetases in combina-tion with NDGA demonstrates that the two enzymes, the synthetase and the acyl-CoA ... synthesis in Y1 cells was significantly inhibited by a synergistic effect of NDGA and triacsin C, an inhibitor of the AA-CoAsynthetase. Our results are consistent with the involvement of an acyl-CoA synthetase ... ACTH on the transcript was inhibited by actinomycin D andenhanced by cycloheximide [32]. Given the obligatory role of the protein in the activation of steroidogenesis through the release of AA,...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... liver. Furthermore, we investigated the speci®city of the human CBG with r espect to the glyconeand aglycone moieties, and in particular characterized the ef®ciency of the enzyme in hydrolysing a ... reduced by 98% in the presence of 4NP-b-D-mannopyranoside (10 mM)].Hydrolysis of xenobiotic glycosides by reCBG The a bility of CBG to hydrolyse a variety of glucosides wasassessed using a ... (1997) The crystal structures of Sinapis alba myro-sinase and a covalent glycosyl±enzyme intermediate provideinsights into the substrate recogn ition and active-site machinery of an S-glycosidase....
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Báo cáo Y học: Transcriptional regulation of erythropoiesis Fine tuning of combinatorial multi-domain elements ppt
... activateHS3 directly. One model for the globin chromatin openingproposes that factor binding at HS3 initiates the process,allowing the spreading of open chromatin, binding of othertrans-acting factors ... recently been exten-sively reviewed [52–54]. The b-globin gene is part of the globin cluster, the genes of which are arranged in the order of their expression during development. Regulation of the b-globin ... stages. Therefore, the interplay among the various transcription factors involvingtheir stage of expression, type of cells expressing them, the combination of factors present at a certain time point...
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