... 5¢-side of the processing stem, gave rise to rRNA with the majority of termini in the mature form, whereas pD3, with amutation on the 3¢-side, gave rise rRNA with the majority of the termini in the ... fractions,respectively. In particular, there was an increase in the relative amount of the fully mature terminus, with aconcurrent decrease of the ) 7 species in the 70S frac-tion as compared with the 50S ... Thus, the observed difference may reflect the limitations in quantifying the peak areas of traceswhen most of the free subunits are in the 70S trace, aswas the case with JB69 throughout the Mg2+titration,and...