... with the theory ofmetabolic supply and demand analysis [24]. This theorypredicts that for biosynthetic pathways, such as thoseleading to the biosynthesis of amino acids or nucleotides, the ... acid, the substrate for CDP-diacylglycerol synthetase [31] and increased resistance to the antibiotic erythromycin. How-ever, the mechanisms for CTP and dCTP involvement inlipid biosynthesis and ... growth, suggestingthat the reduced growth rate is indeed related to the decreased CTP and dCTP pool sizes.DiscussionIn this work, we have modulated the expression of the L. lactis pyrG gene encoding...