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Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt

Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt

Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt

... wepresent the EPR spectroscopic and redox properties of GR-1 chlorite dismutase. Furthermore, we investigated the binding of hydroxide and imidazole to the ferric enzyme and of NO to the ferrous enzyme ... oxoferryl species and hypochlorite. The reaction mechanism of WF10 with hemoproteins may besimilar to the enzymatic reaction of chlorite with chlorite dismutase. Thus, the study of chlorite dismutase ... Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 Peter L. Hagedoorn, Daniel C. de Geus and Wilfred...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... clustered together and distant from IL-20 and IL-24, as recently determined from analysis of the spottedgreen pufferfish genome, imply that the carp sequence isIL-10. The hydropathy analysis also ... IL-10 inhibits the activation of macrophages/monocytes, thereby inhibitingcytokine synthesis, nitric oxide (NO) production and the expression of other costimulatory molecules. Apart from IL-10 [1], ... Hydropathy plot of putative IL-10 proteins from carp, torafugu and human. The x-axis denotes the residue position and the y- axisrepresents hydrophobicity. The hydrophobicity analysis was carriedout...
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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... sequence analysis of HpSDHIn the current work, the aroE gene of H. pylori strain SS1 was cloned by using the genome sequences of H. pylori strains 26695 and J99 as major references.We firstly amplified ... result. The low catalytic effi-ciency of HpSDH may result from the sequence vari-ation in the binding sites of substrate and cofactor.However, in light of its relative enzyme activity,HpSDH ... concentrationwas determined by Bradford assay using bovine serumalbumin as standard.Enzymatic activity assay The enzymatic activity of HpSDH was assayed at 25 °Cbymonitoring the reduction of NADP (or NAD)...
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Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

... the locations of the four reflections (see text).Ó FEBS 2003 Biophysical study of Mycoplasma glycolipid (Eur. J. Biochem. 270) 3275LAL assay The LAL test is based on the property of lysates of amebocytes ... molecules.Gel-to-liquid crystalline phase transition and lipidheadgroup conformations The determination of the b«a gel-to-liquid crystallinephase transition of the acyl chains from the evaluation of the symmetric ... presently cannot answer the questionwhether the cubic structure observed is of the ÔnormalÕ(right side out) or the inverted type, the geometry of the molecule with its bulky headgroup is in favor of...
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Báo cáo Y học: Processing, stability, and kinetic parameters of C5a peptidase from Streptococcus pyogenes pptx

Báo cáo Y học: Processing, stability, and kinetic parameters of C5a peptidase from Streptococcus pyogenes pptx

... [23]. Zymogen conversion to the active enzyme occursby limited proteolysis of the inhibitory pro-sequencesegment and may be either autocatalytic or involve acces-sory molecules. The length of ... and a polyethylene glycol polysty-rene support with a 5-(4¢-Fmoc-aminomethyl-3¢-5¢-dimeth-oxyphenoxy)valeric acid linker. After synthesis, peptideswere purified by reverse-phase HPLC and lyophilized.Homogeneity ... (human), angiotensin I (human), and des-Arg1-bradykinin (synthetic). The mass accuracy was in the range of 0.1%.Hydrolysis of protein and peptide substratesTreatment of the S512A C5a peptidase precursor...
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Báo cáo y học:

Báo cáo y học: "Natural History and Clinical Consequences of Hepatitis B Virus Infection"

... Genotype H was recently identified in individuals from Central America and California [2]. Several genotypes may be associated with the severity of the disease but the relationship between the ... mediated by point mutations with the YMDD motif at the catalytic center of the viral reverse transcriptase. With increase in the mutant viral load, patients can sustain further liver injury. The YMDD ... Pacific Islands and the Arctic and the rate of HBsAg positivity ranges from 8% to 15%. In the low endemic area, such as Western countries, HBV is predominantly a disease of adolescents and adults...
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Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... map and glycan analysis of the naturalb-fructofuranosidaseInvestigation of the carbohydrate moieties of the purifiedallergen from tomato was carried out by MALDI-TOFmass spectrometry. From the ... 2003glycan analysis of the natural protein from tomatorevealed that MMXF is the dominating glycan withabout 84% of all sugar structures. The structures of the N-glycans of nLyc e 2 and their ... hindrance and lowers the IgE reactivity of the xylose epitope. This may be another reason why only asubgroup of sera reacting with glycoproteins recognizednLyc e 2 on the immunoblots.Besides the...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

... Analysis of the promoter region of the cystatin Fgene (CST7) showed that it, like that of the cystatin C gene(CST3), is devoid of typical TATA- and CAAT-box ele-ments. In contrast to the cystatin ... band (lane 3,band a)withMrapproximately 3200 Da higher than the diglycosylated cystatin F species. The nature of this band ispresently unknown and is discussed below.In order to study the ... production of cystatin F comparedto the ubiquitous inhibitor, cystatin C, we measured the cystatin contents in cell lysates and culture media of cellsgrown for 2 and 4 days with cystatin F and cystatin...
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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf

Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf

... 8).Correspondingly, fluorescence of cytoplasmic T4(259–470)–YFP and full-length T4–GFP was already significantly reduced after 2 min of bleaching in a small area of the cytoplasm, whereas the fluorescence of ... transiently transfected with the respective expression plasmids and analyzed by confocalmicroscopy the next day. Full-length GFP-tagged TRAF4molecules were localized in the cytoplasm and were barelydetectable ... is unclearwhether the deleted C-terminal part of the C-TRAF domain of T4(259–387)–YFP and T4(1–307)–YFP is necessary tointeract with the TRAF domain of TRAF4 to allowformation of heteromeric...
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