... modeled the complex by first
placing C5 in the context of the gp120 core structure
determined by X-ray crystallography [7]. The first three
residues of the C5 domain used in the present study
(amino ... in the gp120 X-ray
structure; thus, the C5 domain can be overlaid with the C
terminus of the gp120 X-ray structure (the RMSD of the
NMR VKI ba...
... 2001
geometry, the set of solutions reflects any uncertainty in the
calibration as well as alternative fits to the set of distances.
The main advantages of the procedure are simple: the details
of the ... mobility with respect to
the rest of the structure. Further studies of the receptor
bound forms of the chimera and other related ligands will be
necessary...
... 28–38, connected by a regular
type I b-turn. The surprising similarity of this structure, as
well as the sequence of the C-terminal moiety, with those
of the fusion domain of influenza hemagglutinin ... is the finding that
the sequences of the C-terminal part of Ab-(1–42) and that
of HA_fd share indeed a high similarity (Fig. 6C) dictated
essentially by the pres...
... significantly mod-
ifying the secondary structural elements of the protein.
One consequence of the presence of TFE on the structure
of Vpr is the tertiary structure may open up. However,
the NMR solution ... been
clearly demonstrated by the loss of activity of Vpr
following certain point mutations [16,38,39]. This is one
of the main reasons why the aim of...
... H
N
resonance of Ser4 is
the most low-field shifted amide (Fig. 2).
Solution structure of VVVV2KE
The solution structure of VVVV2KE, represented by the
ensemble of 20 energy-minimized DYANA conformers,
consists ... structures. As
with the NMR structure of VVVV2KE, the N-terminal cap
structure of TTTT includes a hydrogen bond between the
side chain carboxyl g...
... The MBD of MBD1 contacts the methyl
groups of a methylated CpG through a hydrophobic patch
formed by the side chains of five residues, V20, R22, Y3 4,
R44, and S45 [19].
Although the solution structure ... one-turn helix on the other face. It is thought
that the two inner strands of the b-sheet lie within the major
groove of the DNA and that a hydrophobic pocke...
... similarity of the structures. The lowest-energy structure from the
RICH NMR ensemble is used for the overlay. (D) The surface of the RICH catalytic domain shows several negatively charged patches of ... Secondary structure and the two catalytic motifs (*) are
shown. (B) Representation of flexibility in the solution structure of
the RICH catalytic domain....
... back onto the core of the protein thereby allowing the
side chain of Leu86 to partly fill the hole created by the
removal of Ile14. Due to these changes other alterations are
induced in the HPr(I14A) ... displays a kink towards the
interior of the protein. The space that in the wild-type
molecule is occupied by the large hydrophobic side chain of
Fig. 3. Struct...
... little direct
effect on the activity of the enzyme. The function of this
unique C-terminal domain remains unknown.
Overall structure
Crystals of the catalytic domain of hTRXL (hTRXL-N)
were obtained ... pairs of hydrogen bonds are formed in the
active site of hTRXL-N (Fig. 4), accounting for the
compactness and stability of the active site. The H-bond
lengt...
... in the relative
orientations of the a and b domains. In one struc-
ture, the catalytic cysteines face each other; in the
other, the catalytic residues of the a domain face
away from the a¢ domain. ... Superimposition of the solution structure of the human PDI-b¢ (blue) with the crystal structure of yeast PDI-b¢ (red, Protein Data Bank
entry code 2B5E). (...