... Yasuoka S, Onishi T, Kawano S, Tsuchihashi S, Ogaw-ara M, Masuda K, Yamaoka K, Takahashi M & SanoT (1997) Purification, characterization, and localization of a novel trypsin-like protease ... Thisnewly revealed terminus acts as a tethered ligand thatbinds to the receptor and autoactivates it. PAR-2 isactivated by trypsin and mast cell tryptase and isknown to mediate airway inflammation ... cell lysates were prepared as described previ-ously. Each lysate was diluted with bicarbonate-carbonatebuffer, applied to a separate well of a 96-well plate (NUNCMaxisorp) and incubated at 40...