Báo cáo khoa học: Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers pptx
Báo cáo khoa học: Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers pptx
... probability that violaxanthin remains violaxanthin; AA, probability that anthera-xanthin remains antheraxanthin; ZZ, probability that zeaxanthinremains zeaxanthin; SVA, the constant rate of ... conversion of violaxanthin to zeaxanthin is catalysed by violaxanthin de-epoxidase (VDE) and the reverse reaction of violaxanthin formation from zeaxanthin is catalysed by another enzyme, zeaxanthin epoxidase. ... light-dependent de-epoxidation of violaxanthin to zeaxanthin via anther-axanthin as an intermediate and light-independent epoxi-dation of zeaxanthin to anteraxanthin and violaxanthin [2].The conversion of...
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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 fi 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf
... Methyl a- d-glucopyranoside was used as a glucose analog that is not a substrate of hexokinase in a glucose assay system. The data were analyzed on thebasis of the Michaelis–Menten equation; the maximumactivity ... GBd, glucan-binding domain; GS, glucan-binding domain-deficient glucosyltransferase-I; GSd, glucansucrasedomain; GSGB, glucosyltransferase-I containing a full-length glucan-binding domain; GTF, ... sucrose in theabsence of the pre-existing dextran (i.e. in a dextran-independent manner) [14], albeit at a lower rate. Thissuggests that the glucan synthesis reaction catalyzed by the GSd is a basic...
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Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
... (PMNs) at the site of in ammation is accompanied by degranulation and/or lysis of these in am-matory cells resulting in the extracellular release of a variety of hydrolases and oxidases, as well as ... porcine pancreatic elastase; Suc-(Ala)3-p-NA, succinyl-Ala-Ala-Ala-p-nitroanilide; MeO-Suc-(Ala)2-Pro-Val-p-NA, methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide;MeO-Suc-Lys-(pico)-Ala-Pro-Val-TBE, ... may be concludedthat both recombinant elafin and trappin-2 may act aspotent anti -in ammatory molecules and may be of thera-peutic potential in the treatment of various in ammatorylung diseases.Keywords:...
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
... similar constants for theVs/Vhfor deacylation with 7-ADCA and 6-APA using PAAand PGA. However they observed in all cases a saturation of the (Vs/Vh)max, whereas our data indicate a linear ... increase in activity of PA may be caused by small structural changes in the active site at lowconcentrations of D2O, making enzyme–substrate inter-actions more favourable for catalysis than in ... the steady-state rate of hydrolysis was preceded by an exponential burst phasewas p-NPA, indicating that for this substrate the rate of formation of the covalent intermediate is faster than thehydrolysis...
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Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf
... within a multigene family is regulated by transcription factor TFIIIBAkhila Parthasarthy and Karumathil P. GopinathanDepartment of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, ... DNA bind-ing in gel retardation assays using a labelled fragmentcontaining the TATATAA sequence (Fig. 2D, left).Because TBP is present as a component of TFIIIB, theTFIIIB-containing fraction ... N-terminal histidine tagwas purified by binding to and elution from Ni-NTA-affinitymatrix [43].Nuclear extract preparation and fractionation of the B. mori transcription machineryNuclear extracts...
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Tài liệu Báo cáo khoa học: "Integration of Large-Scale Linguistic Resources in a Natural Language Understanding System" pdf
... semantic analysis, and pragmatic analysis. Each stage has been designed to use linguistic data such as the lexicon and grammar, which are maintained separately from the engine, and can easily ... example, the verb abridge has an associated case frame consisting of an agent doing the abridging and an optional theme that is being abridged. Furthermore, in an English sentence using ... Integration of Large-Scale Linguistic Resources in a Natural Language Understanding System Lewis M. Norton, Deborah A. Dahl, Li Li, and Katharine P. Beals Unisys Corporation 2476...
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Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx
... Eda K, KagawaW, Takata M, Takeda S, Yokoyama S & Shibata T(2001) Homologous-pairing activity of the humanDNA-repair proteins Xrcc3.Rad51C. Proc Natl AcadSci USA 98, 5538–5543.20 Kagawa ... structure of Rad54 (Protein DataBank ID: 1Z3I) was obtained from the Protein Data Bankand was employed as a basal structure. A molecular dia-gram was generated using ucsf chimera [48].AcknowledgementsThis ... Experimental proceduresDNA A 74-mer ssDNA (5¢-ACGGGTGGGGTGGACATTGACGAAGGCTTGGAAGACTTTCCGCCGGAGGAGGAGTTGCCGTTTTAATAAGGATC-3¢) (Hokkaido SystemScience, Hokkaido, Japan) and /X174 circular ssDNA(New...
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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
... constructed a pro-tyrosinase mutantcarrying an alanine at this position, F45 3A. Curi-ously, an increase in protein expression was obtainedfor this mutant tyrosinase similar to that obtainedwhen ... tyrosinase constructs without thepredicted N-terminal signal peptide (amino acids1–36), we retained amino acid 36, an alanine, ratherthan using amino acid 37, a lysine, because it is known that, ... pre-pro-tyrosinase (Fig. S1) can be dividedapproximately into three domains: a twin argininetranslocase (TAT) signal peptide, a core domain con-taining the two copper-binding motifs and a C-termi-nal...
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Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx
... promoters indicate the a- F1-ATPase GAF/Adf-1 bindingcassette has enhancer properties. (A) The basal promoter activity of b-F1-ATPase is greatly increased when the a- F1-ATPase GAF/Adf-1 bindingcassette ... a- F1-ATPase gene is more heavily tran-scribed in the head and thorax of adult flies in comparisonto the abdomen (data not shown), indicating that itsexpression is differentially regulated at ... founddownstream of transcriptional initiation sites in Drosophilapromoters include ACGT, ACAA, ACAG, and AACA[32], and these were detected at )17, )18, )36 and )103positions of the a- F1-ATPase gene...
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Tài liệu Báo cáo Y học: Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine docx
... disease. However, CAA is also seen in normal ageing. There is increasing evidence thatCAA may underlie certain forms of vascular dementia andintracranial hemorrhage associated with ageing [1]. ... viability. Ab also caused anincrease in the number of apoptotic cells. This increase in apoptosis was accompanied by activation of caspase-3.Homocysteine, a known risk factor for cerebrovasculardisease, ... cerebrovasculardisease, increased Ab-induced toxicity and caspase-3 acti-vation in a dose-dependent manner. These studies suggestthat Ab may activate apoptotic pathways to cause loss of VSMC in CAA by...
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