Báo cáo khoa học: Role of Ca2+/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions Comparison with the role of phosphotidylinositol-3 kinase ppt

... multiple intracel-lular signaling pathways. Therefore, the role of calmodulin, the major downstream effector of Ca2+, in the regulation of intracellular signaling was analyzed by using the inhibitorW7. ... counteracts the calcineurin induced afﬁnity switch of a5b1integrin in CHO cells,suggesting also a role for Ca2+/calmodulin-dependentmolecules in regulating the afﬁnity state of integrins onneutrophils ... bothcalmodulin and PtdIns3K activity. These data demonstratecritical roles for changes in [Ca2+]iand calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a coupled oscillator-based mechanism in smooth muscle ppt

... 37% of its original size) is typically in the range 2–3 mm [26].Finding experimental evidence that electrical cou-pling is the key ‘spring’ interlinking the Ca2+storeshas involved repeating ... junctions could serve as the springjoining the pendulums in the above analogy. However,coupling through release of Ca2+results in very weakcoupling, as the effective diffusion of Ca2+is limitedto ... asshown in the study of Yamashita [36] (see accompany-ing review), and it will be interesting to determine the relative role of this mechanism. In summary, store-based pacemaking, whether oper-ated...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: involvement of ATP release in astrocytes pdf

... andP2X7 receptors, suggesting the involvement of multiple pathways in the release. In addition to these, the release of ATP is partly dependent on Ca2+[15] andSNARE proteins [16], and astrocytes ... events in the synchronization of SICs [31]. As shown in the rightpanel of Fig. 3B, reactive astrocytes increase the release of glutamate by facilitating Ca2+oscillations in response to the gliotransmitter ... extracellular signaling between astro-cytes and neurons could be involved in the regulation of synaptic transmission both in physiological and in pathophysiological conditions. In this minireview, the...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium pptx

... cytoplasmic domain of cadherin interacts with F-actin via b-catenin and a-catenin; b-catenin bindsto cadherin and a-catenin, which in turn interacts with F-actin (Fig. 3A) [24]. Thus b-catenin plays ... pivotal role in the regulation of cell–cell adhesion. The interac-tion of b-catenin with cadherin is regulated by tyrosinephosphorylation of b-catenin [25,26], which leads todisassembly of the ... cadherin–catenin complexes within eachcell and by cadherin–cadherin interactions between the two cells. The interkinetic cells adhere to each othervia cadherin–catenin complexes, and these...

Báo cáo khoa học: Modulation of Ca2+ entry and plasma membrane potential by human TRPM4b pptx

... kept in serum-containing medium instead of Ringer solution[16]. Interestingly, the gene encoding TRPM4 has beenshown to be one of the genes upregulated during the transition from prostatic intraepithelial ... alteration in Ca2+entry follow-ing store depletion. In uence of TRPM4b on ionomycin -induced membrane depolarizationCa2+entry induced by depletion of intracellular Ca2+stores by ionomycin also induced ... regulator of Ca2+entry. The upregulation of TRPM4b might then be involved in the furtherprogression of the tumor. Such a possible role of ABFig. 3. Ca2+release and Ca2+entry induced by...

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... understanding of the role of various interactingcomponents. The formation of dopamine–quinoneadducts (because of auto-oxidation of the neurotrans-mitter), especially dopaminochrome, with a-synuclein,inhibited ... 1B shows the patternseen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... accumulation of toxic protoﬁbrils of a-synuclein,leading to cell death. In this study, we have determined the nature of aggregates formed in the presence of dopamine when a-synuclein is co-incubated with MPTP...

Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx

... (83%)or LAC5 (83%). All together, recombination induced swapping of amino acids for 94 positions (19% of the residues) in the original LAC3 sequence. The C- andN-termini of the hybrids were 33–37% ... chimera,recombination preserved the integrity of domain D1and the very end part of domain D3, two regions thatinteract precisely in the natural laccase fold (Fig. 5).Because of the bias introduced by linearization ... preferred points of recombination could be those corresponding to maxi-mal identity in the overall alignment of the parentalsequences. However, the short window of recombina-tion found in the 3¢...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... specifically bound to the induction of apoptosis, in agreement with recentfindings [8].Second, the intensity of the ML signal, correspond-ing to TG levels, is increased in irradiated MCF-7cells, ... deconvolution of spectra of the typeshown in Fig. 1A¢, with ML signals of a very lowintensity, was performed as a first step. Deconvolution of spectra of the type shown in Fig. 1A, with intenseML ... confluence.This finding is in agreement with the observed increase of the G1 phase in the final days in culture (Fig. 4A¢,B¢). It is reasonable to assume that cell metabolism,including PL synthesis, slows...

Tài liệu Báo cáo khoa học: Attenuation of cardiac mitochondrial dysfunction by melatonin in septic mice ppt

... giving to the control samples a value ¼ 1after deducting basal and background values. Sepsisresulted in a signiﬁcant increase in the transcription of the mRNA encoding iNOS in heart of iNOS+ ... ni-trosation, of mitochondrial components indirectly [35]. The free radical pathways of ONOO–are mainly initi-ated secondary to the reaction of ONOO– with CO2,leading to the rapid formation of carbonate ... demonstratethese protective effects of aMT in heart mitochondria of septic iNOS+ ⁄ +mice. aMT treatment counteractediNOS expression, reducing the activity of i-mtNOS,increased the activity of the...

Tài liệu Báo cáo khoa học: Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails ppt

... efﬁcient Hfq binding in vivo or that Hfq interacts with the 5¢rpsO RNAfragment which contains the translational operator of the rpsO messenger [37]. The phosphate number at the 5¢ end of the RNAdoes ... enzymes. The Hfq containing DNA fragment was excised with the sameenzymes and ligated into the corresponding sites of pET11cand then transformed into the E. coli strain BL21(DE3).Cells in 10 ... transcribedusing the corresponding reverse primers. The templateused to synthesize the 3¢rpsO RNA containing an inser-tion of 18 A residues between the two hairpins wasobtained after two PCRs using...

Xem thêm