Báo cáo khoa học: Inactivation of annexin II tetramer by S-nitrosoglutathione pot
Báo cáo khoa học: Inactivation of annexin II tetramer by S-nitrosoglutathione pot
... changes in annexin II [48] and may alter the environment of reactive sites of annexin II by GSNO. We tested whether such changesinfluence the GSNO inhibition of the activity of annexin II. AIIt (5 ... theactivities of annexin II tetramer (AIIt), a member of theCa2+- dependent phospholipid-binding protein family.Incubation of purified AIIt with S-nitrosoglutathione (GSNO) led to the inhibition of AIIt-mediated ... themodification of annexin II by GSNO does not affectthe binding of AIIt to membrane. When differentamounts of AIIt were treated with GSNO, no inhibitionwere observed for the binding of AIIt to liposmes...
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Báo cáo khoa học: Inactivation of tyrosine phenol-lyase by Pictet–Spengler reaction and alleviation by T15A mutation on intertwined N-terminal arm docx
... C. freundiiTPL, consequently the l-DOPA productivity of T15Awas at least two-fold higher than that with C. freundiiFig. 5. Inactivation of TPL enzymes by L-DOPA and its reactivation by PLP. ... N-terminalarm of C. freundii TPL. Biochemical characterization of the native and mutant enzyme proved the mutationon the interface increased the stability of the catalyticcapability of the enzyme by preventing ... effect on C. freundii TPL cofactor stability S G. Lee et al.5566 FEBS Journal 273 (2006) 5564–5573 ª 2006 The Authors Journal compilation ª 2006 FEBS Inactivation of C. freundii TPL by Pictet–SpenglerreactionCyclic...
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Tài liệu Báo cáo khóa học: Inactivation of copper-containing amine oxidases by turnover products doc
... cuvette closed by a Teflon stopper. The oxygen was consumed by mostsubstrates within the mixing time as revealed by theimmediate bleaching of the TPQ 500 nm band and by theappearance of absorption ... course of LCAO inactivation by spermidine. LCAO,2.8 lM, was inactivated at 37 °Cby2.0mMsubstrate in 0.1Mpotas-sium phosphate buffer at different pH values (open symbols) and inpresence of ... to identify the modification induced by H2O2, butthe similarity of the behavior of several amine oxidasessuggested that it consists of the oxidation of a conservedresidue at the active...
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Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx
... percentage of cellsthat were annexin V positive (annexin V+⁄ PI–and annexin V+⁄ PI+) by flow cytometry. Plots in the lower panel illustrate the 8 h timepoint. Data are representative of two ... following inducible expression of the consti-tutively active form of IRF-3, IRF-3 5D [14]. Up-regu-lation of ArgII gene expression was observed aftertreatment of the tetracycline inducible ... up-regulation of ArgII was next studied in the con-text of SeV infection, a negative single-strand RNAparamyxovirus known to be a strong activator of IRF-3 phosphorylation [23]. ArgII protein...
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Báo cáo khoa học: Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II ppt
... Department of Biology, Faculty of Science, Tokyo University of Science, Tokyo, Japan2 Institute of Biological Science, University of Tsukuba, Japan3 Department of Biology, Faculty of Science, ... University of Hyogo, for the generous supply of cells of C. parad-oxa, C. gracilis and P. tricornutum. The present workwas supported in part by Grants-in-Aid for ScientificResearch from the Ministry of ... complexknown as photosystem II (PSII). The PSII complexcontains a number of intrinsic proteins and 3–4 extrin-sic proteins associated with the luminal side. So far thePSII membrane fragments and...
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Báo cáo khoa học: Inactivation of phosphorylase is a major component of the mechanism by which insulin stimulates hepatic glycogen synthesis doc
... 2003Stimulation of glycogen synthesis but not activation of glycogen synthase by insulin can be explained by inactivation of phosphorylaseTo determine the role of inactivation of phosphorylase ... activation of hepatic glycogen synthase by insulin involving either inactivation of GSK-3or inactivation of phosphorylase. Insulin phosphorylates and inactivates (–) GSK-3 by activation of protein ... beexplained by activation of glycogen synthaseIn view of the above findings that inactivation of phosphorylase mimics insulin stimulation of glycogensynthesis (Figs 4 and 5), whereas inactivation of...
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Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx
... solu-tion of ThT and the fluorescence intensity of the solu-tion was measured at 482 nm. Figure 4A shows thekinetics of aggregation of a-synuclein in the presence of 100 lm MPTP and the effect of 50 ... shown to inhibit fibrillation of a-synuclein by promoting the formation of nonamyloidogenic oligomers.Fibrillation of a-synuclein is accelerated in the presence of pesticides andthe neurotoxin ... aggregation even in the absence of com-ponents of mitochondrial complex I. In contrast to the effect of dopamineon the aggregation of a-synuclein alone, in the presence of MPTP orMPP+, the aggregates...
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Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx
... thepresence of various concentrations of 2A2 mAb. Thebinding of 2A2 mAb was followed by native gel elec-trophoresis. Increasing the concentration of 2A2 mAbat a fixed molar ratio of cathepsin ... &Bjork I (2000) Cystatin inhibition of cathepsin Brequires dislocation of the proteinase occluding loop.Demonstration by release of loop anchoring throughmutation of His110. FEBS Lett 487, 156–160.40 ... Faculty of Pharmacy, University of Ljubljana, Slovenia2 Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia3 Department of Biology, Biotechnical Faculty, University of Ljubljana,...
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Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... incubated for a period of 30 min at37 °C before the reaction was quenched by the addition of 3.25 mL of methanol ⁄ chloroform ⁄ heptane (10 : 9 : 7) and1.1 mL of 0.1 m potassium carbonate and ... hydrolysis of triolein by purifiedrecombinant rat adipocyte HSL, with a decrease in the effect of bis-ANSupon PKA phosphorylation of HSL. The interaction of HSL with bis-ANSwas found to have a Kd of ... creating high levels of backgroundfluorescence, we investigated the possibility of usinglower amounts of ATP for the phosphorylation of HSL. Using radiolabelled ATP, we showed that, by using only 15...
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Tài liệu Báo cáo khoa học: Attenuation of cardiac mitochondrial dysfunction by melatonin in septic mice ppt
... com-plex II were significantly lower, and those of com-plex III and IV were significantly higher, in iNOS– ⁄ –Fig. 2. Effects of aMT treatment on CLP-induced mRNA levels of iNOS. Ten nanograms of ... regarding ATP production suggestthat the low activities of complex I and II in iNOS– ⁄ –mice are compensated by the higher activities of com-plex III and IV, allowing normal mitochondrialhomeostasis.Mitochondrial ... presence of this enzyme in different tissues with properties of endothelial NOS, nNOS, and ⁄ or iNOS [14,31,32]. Indifferent models of sepsis and tissues, the existence of both mtNOS and i-mtNOS isoforms...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDETrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếThơ nôm tứ tuyệt trào phúng hồ xuân hươngChuong 2 nhận dạng rui roTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ