Báo cáo khoa học: NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube pptx

... NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube Caroline ... showthat NBR1 interacts with two proteins; fasciculation and elongation protein zeta-1 (FEZ1), a P KCf interacting pro -tein, and calcium a nd integrin binding p rotein (CIB), whichis associated with ... analysed using a L SM510 laser scanningconfocal microscope (Zeiss).RESULTS NBR1 interacts with the PKC zeta interacting protein FEZ1 and the calcium and integrin binding protein CIB In order...

Báo cáo khoa học: CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site doc

... receptor-associ-ated protein (RAP), and partially by the C1q -binding protein calreticulin, where inhibition experiments indi-cated that half of the binding sites on C1q for CD91were shared with calreticulin ... share the property of binding the MASPs and therefore do not activate the complementcascade but function as opsonins. The lung surfactantproteins share with MBL and the ﬁcolins a uniquedomain ... 2010 The Authors Journal compilation ª 2010 FEBSligand -binding clusters and an 85 kDa beta-chaininvolved in endocytosis and comprising the transmem-brane and intracellular domains. Ligand interaction...

Báo cáo khoa học: Nucling interacts with nuclear factor-jB, regulating its cellular distribution potx

... factor-jB (p65 and p50) and that the binding domain of Nucling isits amino-terminal region (amino acids 1–466) containing ankyrin repeats.Overexpression of Nucling prevents the translocation ... screening for the protein interacting with Nucling using NuclingDN as ‘bait’ [4]. Therefore, itis conceivable that Nucling is involved in several signal-ing pathways through interactions with ... that Nucling interacts with NF-jB-p50 and possibly with NF-jB-p65 in primary cells.Nucling in uences the localization of NF-jBTo verify the interaction between Nucling and NF-jBproteins in vivo,...

Báo cáo khoa học: UXT interacts with the transcriptional repressor protein EVI1 and suppresses cell transformation ppt

... yeast colonies containingthese plasmids are shown in (B) and (E) onSD plusL-histidine and adenine and (C) and (F) on SD lackingL-histidine and adenine.Evi1 binding proteins R. McGilvray et ... transformed with the combination ofplasmids shown. The growth of single yeastcolonies containing these plasmids areshown in (B) and (E) on SD lackingL-histi-dine and adenine and (C) on SD ... conservation in addition to the CtBP -binding sites, suggesting additional commonactivities might have been conserved during evolutionfor these two proteins.To date, several Evi1 -binding proteins have...

Báo cáo khoa học: Dematin interacts with the Ras-guanine nucleotide exchange factor Ras-GRF2 and modulates mitogen-activated protein kinase pathways doc

... dematincontaining 84 amino acids of the core domain and completeheadpiece domain mediated binding with the DH domain ofhuman Ras-GRF2 (Fig. 1). In contrast, a bait constructcontaining only the ... association ofRas-GRF2 with dematin, an actin binding and bundling protein, provides a potential coupling mechan ism betweenRas signaling a nd the a ctin cytoskeleton without Rho protein intermediaries. ... interaction and to d etermine the binding af® nity between the C-terminal domain of dematin[dematin(224±383)] and GST±Ras-GRF2. The GST±dematin(224±383) fusion protein was af®nity-puri®edusing GSH-Sepharose...

Báo cáo khoa học: IMP1 interacts with poly(A)-binding protein (PABP) and the autoregulatory translational control element of PABP-mRNA through the KH III-IV domain pdf

... plotted against the amount of the protein. The binding constant was calculated by determining the protein molar concentration at 50% binding efﬁciency [33].Fig. 4. Simultaneous binding of PABP and ... nm and 5.9 nm, respectively. The IMP1 domain responsible for binding to the ARSIMP1 is a modular protein with two RRM type and four KH RNA binding domains. To examine which of the six RNA binding ... poly(A) -binding protein (PABP) mRNA translation involves the formation of a heterotrimeric ribonucleoprotein complex by the binding of PABP, insulin-like growth factor II mRNA binding protein- 1...

Báo cáo khoa học: Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome ppt

... stabili-ties of interaction with the pre-60S complex and notinefﬁcient binding to the column. In order to determine whether the Nop53p trun-cated mutants also bind RNA directly, in vitro RNA binding assays ... [31]. In order to determine whetherNop53p interacts with the pre-rRNA early duringtranscription, chromatin immunoprecipitation (ChIP)experiments were performed, using the fusion protein protein ... RNA sequencespeciﬁcity in in vitro RNA binding experiments. Simi-larly, Nop9p, another example of an RNA -binding protein involved in pre-rRNA processing, associates with 20S pre-rRNA but does...

Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

... signatures in the sequences are present in both proteins, tyrosinases and catechol oxidases; these include the six histidine resi-dues involved in the binding of a pair of copper ions and other conserved ... in copper binding. Preceding the sixth histidine(H3Baccording to the nomenclature used in [6]), at the end of the CuB -binding site, tyrosinase-like enzymesshow an important variation. Usually there ... tyrosinases, including the CuA and CuB binding sites to ligand the copperTable 3. Kinetic parameters for the two PPOs. The enzymes wereobtained from extracts of R. solanacearum strains mutated in...

Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc

... are noclashes of amino acid side chains in the core of the structure, and most mutations occur on the protein surface. The FAD binding groove is conserved with most ligand binding residues identical. ... upon cysteine exchange are the blackappearance of the C30S protein and the orange colour of the C130S mutant. Changing the other cysteine within thesepairs did not result in matching colour ... in vitro or in vivo. This phenotype is in agreement with the ﬁnding that the C130–C133 pair is part of the primary redox-activecentre.Cysteine 159 and 176 stabilize the FAD -binding domainThe...

Tài liệu Báo cáo khoa học: Amprenavir complexes with HIV-1 protease and its drug-resistant mutants altering hydrophobic clusters docx

... hydrophobic interactions in proteins [26]. Removing a methyl group in the protein hydrophobic core affects protein folding and decreases the protein stability in mutant proteins [27]. In PR,two ... Exploring the changes in PR due to binding of two different inhibitors will give insight into the mechanisms of resistance and help in the design ofnew inhibitors.ResultsAPV inhibition of PR and ... PRI54V(greenline). (B) Comparison of the ﬂap regions in the structures. The complexes with APV are in cyan, and the complexes with SQV are in gray. The arrow indicates the shiftsbetween Caatoms at the...

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