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Báo cáo khoa học: Characterization of glycosphingolipids fromSchistosoma mansoni eggs carrying Fuc(a1±3)GalNAc-, GalNAc(b1±4)[Fuc(a1±3)]GlcNAc-and Gal(b1±4)[Fuc(a1±3)]GlcNAc- (Lewis X) terminal structures pot

Báo cáo khoa học: Characterization of glycosphingolipids fromSchistosoma mansoni eggs carrying Fuc(a1±3)GalNAc-, GalNAc(b1±4)[Fuc(a1±3)]GlcNAc-and Gal(b1±4)[Fuc(a1±3)]GlcNAc- (Lewis X) terminal structures pot

Báo cáo khoa học: Characterization of glycosphingolipids fromSchistosoma mansoni eggs carrying Fuc(a1±3)GalNAc-, GalNAc(b1±4)[Fuc(a1±3)]GlcNAc-and Gal(b1±4)[Fuc(a1±3)]GlcNAc- (Lewis X) terminal structures pot

... Schistosoma mansoni egg glycolipids (Eur. J. Biochem. 269) 493 Characterization of glycosphingolipids fromSchistosoma mansoni eggs carrying Fuc(a1±3)GalNAc-, GalNAc(b1±4)[Fuc(a1±3)]GlcNAc-and Gal(b1±4)[Fuc(a1±3)]GlcNAc- ... University of Giessen, Germany;2School of Biological Sciences, University of Wales, Bangor, Wales, UKThe carbohydrate moieties of glycosphingolipids f rom eggs of the human parasite, Schistosoma mansoni, wereenzy-matically ... oligosaccha-rides from S. mansoni eggs. (A) ESI MS and(B) MALDI-TOF MS of complex S. mansoni egg glycosphingo lipids. (C) MALDI-TOF MS of olig osaccharides re leased from S. mansoni egg g lycosphingolipids.484...
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Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... 106mL)1. Next, 0.5 mL of propidium iodide staining solution (10 mg of propidiumiodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of NaCl ⁄ Pi) was added to 0.5 mL of cell suspension. The ... wereanalysed in terms of production ⁄ consumption of TG.In mammalian cells, TG may be deleted to meet theneeds of cell metabolism, either as a source of energyor as a reservoir of fatty acids, after ... otherstudies have not [8,9].A number of studies have been performed in view of the possible use of MLs as spectroscopic markers of cell fate, although a clear explanation of their behav-iour has not...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... addi-tional C -terminal sequence of about 90 amino acids of unknown function. The high degree of identity of theCg-Clp1 and Cg-Clp2 Glyco_18 domains (84% iden-tity) argues for a conservation of the ... an additional 90 amino acid C -terminal sequence of unknown function (Fig. 1). Hence, theoverall structure of Cg-Clp2 is similar to that of Cg-Clp1.Expression of Cg-Clp2 transcripts duringdevelopment ... multiplicity of CLPsin humans and insects [14], we hypothesized thathomologues of the previously characterized Cg-Clp1remain to be found in C. gigas.In this article, we report the characterization of...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... followed by mixture with6 lL of Lipofectamine Plus reagent at room temperaturefor 10 min. Four microliters of Lipofectamine Plus reagentwere diluted in 100 lL of Opti-MEM I-reduced serum med-ium. ... Department of Legal Medicine and Medical Genetics, Gunma University, Japan2 Division of Medical Genetics and Biochemistry, University of Fukui, Japan3 Division of Legal Medicine, University of Fukui, ... patients with stable angina pec-toris, offers an in vivo model of mild myocardial ische-mia in humans. Irrespective of a lack of alteration inlevels of other conventional cardiac markers such...
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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... the mechanism of action of PPI1.The analysis of the pH dependence of PPI1-induced activation of the H+-ATPase showed thatstimulation decreases dramatically with the increase of pH above pH ... withPPI1588His6.The analysis of the effect of PPI1588His6on thedependence of PM H+-ATPase activity on the concen-tration of MgATP (Fig. 3) showed that stimulationdecreased with the increase of PPI1588His6concentra-tion. ... action of the enzyme C-terminus, butfurther enhances the activity of the enzyme whoseC-terminus has been displaced by low pH or byFC-induced binding of 14-3-3s.ResultsThe C-terminus of isoform...
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Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... fromTritirachium album Limber. Characterization of thechromosomal gene and expression of the cDNA inEscherichia coli. Eur J Biochem 179, 185–194. Characterization of a Serratia proteinase K-like ... Willassen1,21 Department of Molecular Biotechnology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, Norway2 The Norwegian Structural Biology Centre, University of Tromsø, Norway3 ... nature.Identification and analysis of the peptidase geneDegenerate primers were constructed on the basis of multiple sequence alignment of proteinase K-like Characterization of a Serratia proteinase K-like...
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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... of all of these genes remains to be clarified.Expression and purification of Sno1p and Snz1pIn light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... with alibrary of yeast chromosomal DNA. It was found that 4.6and 5.1 kb overlapping fragments of chromosome XIII carrying SNO1 and SNZ1 were capable of complementingthe defect of mutant K64 ... absorbance of the supernatant was read at 363 nm. The absorbance of the reduced form of APAD (APADH) was linear over the2–100 lMrange of glutamate with a molar extinctioncoefficient of 8900M)1Æcm)1[17].Detection...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... differencesbetween the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation⁄ desensitiza-tion [20–23] and patterns of expression [24], this repre-sents a question of potentially ... orhypertrophic growth of VSM [5,6], inhibition of angio-genesis ⁄ neo-vascularization [7] and apoptosis of CD4 ⁄ CD8++⁄ –thymocytes [8]. Alterations in the level of this potent autocoid, or of its specific ... (2002) Characteri-zation of the 5¢ untranslated region of alpha and betaisoforms of the human thromboxane A2 receptor (TP).Differential promoter utilization by the TP isoforms.Eur J Biochem 269,...
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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... data.Data analysesData were analysed by means of sigmaplot 2001 (SPSSScience Software Gmbh, Erkrath, Germany). Data for the characterization of the kinetics of electrogenic BSP uptakefitted the Michaelis–Menten ... process.litre/osmol01234nmoles of BSP disappeared0,00,40,81,21,6Fig. 2. The dependence of valinomycin-induced disappearance of BSP on the osmolarity of the extra-vesicular medium in the pres-ence of carnation ... presence or in the absence of variousconcentrations of a ligand A, k2and k1are therate constants of the inhibition of the bilitranslocase–bilirubin complex and of free bilitranslocase, respect-ively....
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Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... of ICAMtransfectants to 0.4 lg of I domain GSTcaptured with 0.2 lg of coated anti-GST Ab.Controls include wells with captured GST andthe binding of wild-type L cells. Backgroundbinding of ... Inhibition of adhesion of ICAM trans-fectants to purified CD11b I domain GSTfusion protein. The effects of anti-ICAM andanti-I domain mAbs on adhesion of ICAMtransfectants to 0.4 lg of I domain ... ICAM-1 transfectants. The presence of both MgCl2and CaCl2gives better binding of ICAM-2 L cell transfect-ants to the I domain of CD11b as well as for adhesion of ICAM-4 transfectants and red...
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