Báo cáo Y học: Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes potx
Báo cáo Y học: Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes potx
... Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes Egon Persson1and Ole H. Olsen21Haemostasis Biology and2Medicinal ... incubation with cyanate.RESULTSEnzymatic activity of FVIIa variantsTwo forms of enzymatic activity are analyzed. The amido-lytic activity is measured to assess the functional status of the active ... mutations gives the most pro-found effect on all these parameters.Keywords: factor VIIa variant; factor X activation; intrinsicactivity; superactivity; zymogenicity. Coagulation factor VIIa...
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Báo cáo y học: "Advances in Molecular Diagnosis of HBV Infection and Drug Resistance"
... assays make use of differing technologies and can generally be divided into (i) signal amplification assays (liquid phase hybridization, antibody capture approach, branched DNA) and (ii) DNA ... ddNTPs by means of a DNA polymerase [34]. An alternative approach involves differential hybridization to a preexisting panel of membrane-bound probes as illustrated by the commercially available ... use of a genotyping assay for polymerase mutant detection and quantitative detection of viremia with a highly sensitive assay is warranted for optimal monitoring of HBV antiviral therapy [42,43]....
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Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
... acids 7–22 of parabutoporin (an a- helical part havingthe four amino acids LAKK identical to mastoparan) and of the first 28 amino acids of the opistoporins were synthesizedand were investigated ... sequence was unambiguously determined byEdman degradation up to amino acid 42. At position 43 a very weak signal corresponding to a proline appeared. Eachsequencing cycle yielded a single clear amino ... cefuroxime and erythro-mycin. Parabutoporin and opistoporin 1 enhance theantibacterial activity of cefuroxime. Mastoparan shows nosynergism with any of the tested antibiotics againstL. monocytogenes....
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Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx
... andsequence analysis of the lipase and lipase chaperone- encodinggenes from Acinetobacter calcoaceticus RAG-1, and redefinition of a proteobacterial lipase family and an analogous lipase chaperonefamily. ... dark blueband. Positive indicator of tricaprylin hydrolysis wasrecorded by appearance of a zone of clearing.Effect of pH on lipase activity and stabilityConcentrated lipase preparations were ... thatcatalyze the hydrolysis of triacylglycerols to free fatty acidsand glycerol. They resemble esterases in catalytic activity,but differ in that their substrates are water-insoluble fatscontaining...
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Báo cáo Y học: Puri®cation and properties of an alkaline proteinase of Fusarium culmorum doc
... connected the amino acidsleucine and aspartic acid. Such bonds are not normallycleaved by trypsin, but are by subtilisin. This indicates thatthese peptides were probably separated by an autolytic e ... for SAAPFpNA. To de®ne the substrate speci®city of the enzyme, its activitywas measured in duplicate with the substrates SAAPFpNA,N-succinyl-Ala-Ala-Pro-Leu pNA (SAAPLpNA), N-gluta-ryl-L-Phe ... enzyme was freeze-dried and submitted to the Protein Chemistry Laboratory of the University of Texas Medical Branch Cancer Center, Galveston, USA, for amino- acid sequence analysis. The enzyme was...
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Báo cáo Y học: The cold-active lipase of Pseudomonas fragi Heterologous expression, biochemical characterization and molecular modeling pdf
... cold-active lipase of Pseudomonas fragiHeterologous expression, biochemical characterization and molecular modelingClaudia Alquati, Luca De Gioia, Gianluca Santarossa, Lilia Alberghina, Piercarlo ... lipases is the so-calledoxyanion hole, an arrangement of amino acids which playsan important role in the stabilization of the tetrahedralintermediate transiently formed in the hydrolysis reactionthrough ... where a restriction site for HindIII was inserted.Reaction was carried out in a total volume of 100 lLandwascatalyzed by 2.5 U of Pfu TurboTM polymerase (Stratagene,CA, USA). The amplification...
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Báo cáo y học: "Facing medical care problems of victims of sexual violence in Goma/Eastern Democratic Republic of the Congo"
... oxygen saturation, materials to administer neuro-axial anaesthesia, and materials for air-way management. Availability of drugs (including expirydates), laboratory tests (including expiry dates), ... using a predefined systematic protocol:number of beds, administrative background, availability of a physician specialized in gynaecological surgery orgynaecology, anaesthetist, psychiatrist ... half of the hospitals had physicians specializedin gynaecology or gynaecological surgery available. Similarly, anaesthetists and psychiatrists/psychologists wereavailable in two (50%) and one...
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Báo cáo y học: "Excess circulating angiopoietin-2 is a strong predictor of mortality in critically ill medical patients"
... rate; m = male; MAP = mean arterial pressure; PaO2 = partial pressure of oxygen in arterial blood; SOFA = Sequential Organ Failure Assessment; VEGF = vascular endothelial growth factor. Available ... the same investigator.Quantification of circulating Ang-1 and Ang-2Ang-1 and Ang-2 were measured by in-house Immuno Radio-metric Sandwich Assay (IRMA) and ELISA, respectively aspreviously described ... using a sandwich ELISA kitaccording to the manufacturer's instructions (R&D Systems,Minneapolis, USA). This assay measures biologically activeVEGF121 and VEGF165.Statistical analysisDifferences...
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Báo cáo y học: " Derivation and preliminary validation of an administrative claims-based algorithm for the effectiveness of medications for rheumatoid arthritis"
... global scores independent of RA disease activity. As part of a sensitivity analysis, we restricted the cohort to patients without any of these ICD-9 codes as part of a sensitivity analysis. As ... provide a preliminary mechanism to evaluate the effectiveness of RA medications in administrative claims and pharmacy data. While clinical disease activity measures remain the gold standard for assessing ... patients had rheumatologist-confirmed RA and well-characterized measures of RA disease activity. The novel linkage between the registry and national VHA administrative data made developing and...
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Báo cáo y học: "Translational Medicine and Reliability of Single-Nucleotide Polymorphism Studies: Can We Believe in SNP Reports or Not"
... Cancer Res. 2004 Apr 15;10(8):2618-25 47. Okuno T, Tamura T, Yamamori M, Chayahara N, Yamada T, Miki I, Okamura N, Kadowaki Y, Shirasaka D, Aoyama N, Nakamura T, Okumura K, Azuma T, Kasuga ... polymorphism and survival of patients with meta-static colorectal cancer. J Natl Cancer Inst. 2002;94:936-42 61. Sreeja L, Syamala V, Hariharan S, Syamala VS, Raveendran PB, Sivanandan CD, Madhavan ... prognosticators and genomic instability in papillary thyroid cancer. Laryngoscope. 2005;115:1479-85 57. Ruzzo A, Graziano F, Kawakami K, Watanabe G, Santini D, Cata-lano V, Bisonni R, Canestrari...
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Từ khóa: Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ